Abstract The precise roles of the B‐box zinc finger family transcription factors in plant stress are poorly understood. Functional analysis was performed on AtCOL4, an Arabidopsis thaliana L. CONSTANS‐like 4 protein that is a putative novel factor, and which contains predicted transcriptional activation domain. Analyses AtCOL4 promoter‐β‐glucuronidase (GUS) construct revealed substantial GUS activity whole seedlings. expression strongly induced by abscisic acid (ABA), salt, osmotic stress....

Functional analysis of a putative novel transcription factor Arabidopsis MYB-like protein designated AtMYBL, which contains two predicted DNA-binding domains, was performed. The physiological role the R-R-type has not been reported in any plant. Analyses an AtMYBL promoter–β-glucuronidase (GUS) construct revealed substantial gene expression old leaves and induction GUS activity by ABA salt stress. AtMYBL-overexpressing plants displayed markedly enhanced leaf senescence phenotype. Moreover,...

Prolamin-containing protein bodies in maize endosperm are composed of four different polypeptides, the α-, β-, γ-, and δ-zeins. The spatial organization zeins within body, as well interactions between them, suggests that localized synthesis γ-zeins could initiate target body formation at specific regions rough endoplasmic reticulum. To investigate this possibility, we analyzed distribution mRNAs encoding 22-kD α-zein 27-kD γ-zein proteins on cisternal reticulum membranes. In situ...

Abstract Defective endosperm* (De*)-B30 is a dominant maize (Zea mays) mutation that depresses zein synthesis in the developing endosperm. The mutant kernels have an opaque, starchy phenotype, malformed protein bodies, and highly increased levels of binding other chaperone proteins Immunoblotting revealed novel α-zein De*-B30 migrates between 22- 19-kD bands. Because maps cluster genes, we characterized cDNA clones encoding these from endosperm library. This led to identification which...

Pesticide delivery carriers provide protection against pathogens but face significant challenges, including limited loading capacity, rapid release rates, and inconsistent performance. To address these issues, this study develops a dual-stimuli-responsive pesticide carrier, featuring an iron-copper bimetal-organic framework (Fe-Cu MOF) supported on diatomaceous earth (DE) coated with lauric acid (LA). Here, DE serves as biocompatible scaffold, enhancing the adhesion retention of MOF...

Mucronate (Mc) was identified as a dominant maize (Zea mays L.) opaque kernel mutation that alters zein storage protein synthesis. Zein bodies in Mc endosperm are misshapen and associated with increased levels of ER Lumenal Binding Protein (BiP). Using GeneCalling to profile RNA transcripts, we an aberrant encodes the 16-kDa gamma-zein protein. The transcript contains 38-bp deletion (nucleotides 406-444 after initiation codon) creates frame-shift abnormal sequence for last 63 amino acids....

• We identified and functionally characterized the AtSKIP gene (At1g77180), an Arabidopsis homologue of SNW/SKIP, under abiotic stresses. Although SNW/SKIP protein has been implicated as a critical transcription cofactor, its biological functions have yet to be reported in any plant. Recently, we isolated Salt-tolerance genes (SATs) via overexpression screening yeast with maize cDNA library. One selected (SAT2) appeared confer elevated tolerance salt. Maize SAT2 encodes human transcriptional...

CCCH-type zinc finger proteins are important for developmental and environmental responses. However, the precise roles of these in plant stress tolerance poorly understood. Arabidopsis thaliana Oxidation-related Zinc Finger 2 (AtOZF2) (At4g29190) is an AtOZF1 homolog previously isolated from Arabidopsis, which confers oxidative on plants. The AtOZF2 protein localized plasma membrane, as AtOZF1. Disruption expression led to reduced root length leaf size. was implicated be involved ABA...

Proline (Pro) metabolism plays important roles in protein synthesis, redox balance, and abiotic stress response. However, it is not known if cross-talk occurs between proline brassinosteroid (BR) signaling pathways. Here, an Arabidopsis intergenic enhancer double mutant, namely content alterative 41 (pca41), was generated by inserting a T-DNA tag the thaliana ring zinc finger 1 (atrzf1 ) mutant background. pca41 had inserted at site of gene encoding BES1/BZR1 Homolog 3 (BEH3). has...

Abstract The nutritional value of maize (Zea mays) seed is most limited by its protein quality because storage proteins are devoid the essential amino acid lysine (Lys). Lys content kernel can be significantly increased opaque-2 mutation, which reduces zein synthesis and increases accumulation that contain Lys. Elongation factor 1α (eEF1A) one these proteins, concentration highly correlated with endosperm. We investigated genetic regulation eEF1A basis for relationship other Lys-containing...

Shoot branching and growth are controlled by phytohormones such as auxin other components in Arabidopsis. We identified a mutant (igi1) showing decreased height bunchy patterns. The phenotypes reverted to the wild type response RNA interference with IGI1 gene. Histochemical analysis GUS assay revealed tissue-specific gene expression anther showed that levels of apical parts, including flowers, were higher than parts plants. biosynthesis component gene, CYP79B2, was up-regulated igi1 mutants...

Proline metabolism is important for environmental responses, plant growth, and development. However, its precise roles in abiotic stress tolerance are not well understood. Mutants valuable the identification of new genes elucidating their physiological mechanisms. We applied a suppressor mutation approach to identify novel involved regulation proline Arabidopsis. Using atrzf1 (Arabidopsis thaliana ring zinc finger 1) mutant as parental line activation tagging mutagenesis, we selected several...

Abstract Cordyceps militaris, an entomopathogenic ascomycete, produces edible medicinal mushrooms known to have and therapeutic functions. To develop the genetic transformation system in C. green fluorescent protein (GFP) mutants of militaris were generated by PEG-mediated protoplast transformation. The CRISPR/Cas9 ribonucleoprotein (RNP) targeting class III histidine kinase (CmHk1) was then delivered into protoplasts through system. Mutations induced RNP selected detected: 1 nt deletion (6...

Developing artificial plant root models to mimic water absorption using biomaterial-derived inks for three-dimensional (3D) printing is challenging because of their rheological behavior and biocompatibility. Herein, we developed optimized a cellulose acetate (CA) ink its parameters extrusion-based 3D-printing fabricate an object that mimics the mechanical properties ability roots. The composition CA were correlated enabling uninterrupted structures with layer thickness 0.1 mm high shape...

Sclerotinia sclerotiorum is the causal agent of sclerotinia stem rot in over 400 plant species. In a previous study, group III histidine kinase gene S. (Shk1) revealed its involvement iprodione and fludioxonil sensitivity osmotic stress. To further investigate fungicide associated with high-osmolarity glycerol (HOG) pathway, we functionally characterized SsHog1, which downstream Shk1. generate knockout mutants, split marker transformation combined newly developed repeated protoplasting...