A5101428462- Nitric Oxide and Endothelin Effects
- Gastric Cancer Management and Outcomes
- Cancer Immunotherapy and Biomarkers
- Gastrointestinal Tumor Research and Treatment
- Blood Pressure and Hypertension Studies
- Lung Cancer Treatments and Mutations
- Sepsis Diagnosis and Treatment
- Pulmonary Hypertension Research and Treatments
- Neuroscience of respiration and sleep
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Eicosanoids and Hypertension Pharmacology
- Esophageal and GI Pathology
- Heart Rate Variability and Autonomic Control
- Metastasis and carcinoma case studies
- Lung Cancer Research Studies
- Colorectal Cancer Surgical Treatments
- Pancreatic and Hepatic Oncology Research
- Abdominal vascular conditions and treatments
- Gastrointestinal disorders and treatments
- Helicobacter pylori-related gastroenterology studies
- Esophageal Cancer Research and Treatment
- Renin-Angiotensin System Studies
- Nutrition and Health in Aging
- Adipose Tissue and Metabolism
- Cholangiocarcinoma and Gallbladder Cancer Studies
Hidaka Hospital
2024
Wakayama Medical University
2017-2023
Juntendo University Shizuoka Hospital
2013-2023
Naga Municipal Hospital
2023
Ehime University
2014-2015
Land Stewardship Project
2014
Hosei University
2013
Juntendo University
1995-2012
Kihoku hospital
2003-2011
Nagoya City University Hospital
1995-2011
ObjectivesPatients treated with nivolumab often experience its unique adverse events, called immune-related events (irAEs). Regarding the mechanisms of immune-checkpoint inhibitors (ICIs), occurrence irAEs may also reflect antitumor responses. Here, we report clinical correlation between and efficacy in NSCLC patients nivolumab.Materials methodsBetween December 2015 February 2017, 38 advanced were our institution. All enrolled single-institutional, prospective, observational cohort study...
In rat aortic strips, muscle contraction was recorded simultaneously with cytosolic Ca++ level, which indicated by the 500 nm fluorescence of indicator, fura 2, due to excitation at either 340 (F340) or 380 (F380) and ratio F340 F380 (R340/380). On addition 72.7 nM K+ 1 microM norepinephrine, followed increase in R340/380 (resulted from increased decreased F380). Cytosolic concentrations resting, mM K+-stimulated norepinephrine-stimulated aortas were tentatively calculated as 228 +/- 25,...
In rat aortic strips loaded with a Ca2+ indicator, fura 2, the signal and contractile tension were simultaneously recorded. On addition of high K+ or noradrenaline, 500 nm fluorescence produced by excitation at 340 increased that 380 decreased. EGTA decreased in resting stimulated muscles. Tension changes always preceded fluorescent changes.
Nitric oxide plays an important role in modulating pulmonary vascular tone. All three isoforms of nitric synthase (NOS), neuronal (nNOS, NOS I), inducible (iNOS, II), and endothelial (eNOS, III), are expressed the lung. Recent reports have suggested for eNOS modulation tone chronically; however, relative contribution to acute is uncertain. We therefore tested effect targeted disruption each isoform on reactivity transgenic mice. Isolated perfused mouse lungs were used evaluate selective loss...
In the pulmonary artery isolated from 1-week hypoxia-induced hypertensive rats, endothelial NO production stimulated by carbachol was decreased significantly in situ visualization using diaminofluorescein-2 diacetate and also cGMP content. This change followed decrease carbachol-induced endothelium-dependent relaxation. Protein expression of synthase (eNOS) its regulatory proteins, caveolin-1 heat shock protein 90, did not hypoxic artery, indicating that chronic hypoxia impairs eNOS activity...
To investigate the role of endothelin-1 (ET-1) in pathogenesis hypoxic pulmonary hypertension, we studied effects a recently described endothelin-receptor antagonist (ETA), BQ123, on development this process. Intraperitoneal osmotic pumps were placed into 8-wk-old Sprague-Dawley rats that received either saline or BQ123 (0.15 mg/h). The maintained room air normoxia hypobaric chamber (380 Torr) for 2 wk to induce hypertension. There no hemodynamic differences between normoxic treated with...
Disseminated intravascular coagulation (DIC) has been recognized as an urgent and critical condition in patients with sepsis. Therefore, unfamiliar time-consuming tests or a complex scoring system are not suitable for diagnosis. Sepsis-induced coagulopathy (SIC), newly proposed category delineated by few global tests, established early warning sign DIC. The purpose of this study was to elucidate the characteristics SIC, especially relation score International Society on Thrombosis...
To evaluate the role of endothelin (ET) receptors in ET-1-induced pulmonary vasoreactivity, we studied effects ET-receptor agonists and antagonists isolated perfused rat lungs. ET-1 (1-10 nM) caused concentration-dependent vasoconstriction gross edema at a concentration 10 nM. The combination selective ETA antagonist BQ-123 ETB BQ-788 inhibited more effectively than alone, whereas alone enhanced constriction. hydrostatic was prevented by but not either or alone. After addition 125 ng...
With advances in the treatment of sepsis, systemic inflammatory response syndrome (SIRS) has been losing its prognostic power. Since SIRS category is no longer used for diagnosis disseminated intravascular coagulation (DIC) diagnostic criteria released by Japanese Association Acute Medicine (JAAM) should be modified. Thus, purpose this study was to examine appropriateness replacing score with antithrombin activity JAAM-DIC criteria. We analyzed data from 819 septic patients who had received...
Sepsis-associated disseminated intravascular coagulation (DIC) carries a high risk of death. Thus, simple tool to quickly establish DIC diagnosis is required. The purpose this study was introduce the and reliable for prediction outcome in patients with sepsis complicated by coagulopathy. We investigated performance simplified Japanese Society on Thrombosis Hemostasis (JSTH) diagnostic criteria. In study, we conducted retrospective, multicenter survey 107 general emergency critical care...
Cytosolic free calcium concentration ([Ca2+]i) and muscle tension were simultaneously measured in aortic tissue isolated from spontaneously hypertensive rats (SHR), normotensive Wistar-Kyoto (WKY) rats, SHR chronically treated with a novel angiotensin converting enzyme inhibitor, CS-622. In the presence of 2.5 mM Ca2+ bathing solution, [Ca2+]i fura-2 was higher than WKY it almost same CS-622-treated untreated rats. Increase external zero to elicited contraction aortas but not both When...
The endothelium modulates vascular contractions. We investigated the effects of oxidative stress on endothelial modulation contractions in hypertension.Changes isometric tension femoral arterial rings from spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats were recorded.The contractile response to norepinephrine arteries with was greater SHR than WKY (P < 0.0001). Endothelium removal augmented norepinephrine-induced contraction 0.05). augmentation more pronounced SHR, which...
We evaluated the role of cross‐linking FcεRI‐mediated inositol 1,4,5‐triphosphate (IP 3 ) in increase cytosolic Ca 2+ level ([Ca ] i using xestospongin C, a selective membrane permeable blocker IP receptor, RBL‐2H3 mast cells. In cells sensitized with anti‐dinitrophenol (DNP) IgE, DNP‐human serum albumin (DNP‐HSA) and thapsigargin induced degranulation β‐hexosaminidase sustained [Ca . Xestospongin C (3 – 10 μ M inhibited both these changes that were by DNP‐HSA without changing those...
The increased production of reactive oxygen species plays a role in the etiology hypertension, but effects antioxidants on blood pressure are controversial. However, possibly lower elderly patients with because vascular aging is also closely related to oxidative stress. Effects chronic treatment ascorbic acid (CAS 50-81-7; 600 mg/day for 6 months) and levels C-reactive protein, 8-isoprostane, malondialdehyde-modified low-density lipoproteins were examined (n = 12, six males/six females, age...
Although programmed death-ligand 1 (PD-L1) expression on tumor tissue is a validated predictive biomarker for PD-1 pathway blockade in non-small cell lung cancer (NSCLC), longitudinal changes its during treatment remains elusive. Circulating cells (CTCs) are assumed to reflect the transition of characteristics primary undergoing anticancer treatment. Here, we sequentially evaluated PD-L1 CTCs NSCLC patients treated with nivolumab. Forty-five were enrolled, and enriched from 3 mL peripheral...