Abstract Spiders are representative arthropods of adaptive radiation. The high-quality genomes have only been reported in several web weaver spider species, leaving the wandering spiders’ genomic information scarce. pond wolf spider, Pardosa pseudoannulata , is a species retrolateral titial apophysis (RTA) clade. We present chromosome-level P. pseusoannulata genome assembly 2.42 Gb size with scaffold N50 169.99 Mb. Hi-C scaffolding assigns 94.83% bases to 15 pseudo-chromosomes. repeats...

CYP6ER1 overexpression is a prevalent mechanism for neonicotinoid resistance in Nilaparvata lugens. Except imidacloprid, the metabolism of other neonicotinoids by lacked direct evidence. In this study, knockout strain (CYP6ER1-/-) was constructed using CRISPR/Cas9 strategy. The CYP6ER1-/- showed much higher susceptibility to imidacloprid and thiacloprid with an SI (sensitivity index, LC50 WT/LC50 CYP6ER1-/-) over 100, which 10-30 four (acetamiprid, nitenpyram, clothianidin, dinotefuran) less...

The cockroach, Periplaneta americana, is an obnoxious and notorious pest of the world, with a strong ability to adapt variety complex environments. However, molecular mechanism this adaptability mostly unknown. In study, genes microbiota composition associated adaptation were studied by analyzing transcriptome 16S rDNA pyrosequencing P. americana midgut, respectively. Midgut analysis identified 82,905 unigenes, among which 64 putatively involved in digestion (11 genes), detoxification (37...

Insecticide synergists are key components to increase the control efficacy and reduce active ingredient use. Here, we describe a novel insecticide synergist with activity specific for insecticidal neonicotinoids. The IPPA08, cis configuration neonicotinoid compound unique oxabridged substructure, could toxicity of most insecticides belonging Resistance Action Committee (IRAC) 4A subgroup against range insect species, although IPPA08 itself was almost inactive insects at synergistic...

The importance of glutathione S-transferases (GSTs) in imidacloprid resistance Nilaparvata lugens, a major rice pest, and other insects was often excluded, mostly due to the slight effects diethyl maleate (DEM) on synergizing resistant populations. Here, we found that synergistic DEM were time-dependent. At 24 or 48 h, time selected record mortalities bioassay, really did not cause an obvious increase toxicity. However, significant observed after 72 h. results revealed GSTs, as phase II...

Paraisaria gracilioides (Kobayasi) Luangsa-ard, Mongkolsamrit & Samson, originally described as Ophiocordyceps gracilioides, is a member of the Ophiocordycipitaceae, within order Hypocreales. This study, presented mitochondrial genome P. structured circular molecule spanning 181,140 bp, was larger than those most Ophiocordycipitaceae species. Despite this size variation, encoding 49 genes, including 15 PCGs, 26 tRNAs, two rRNAs, and six ORFs. Phylogenetic analysis based on nucleotide...

<title>Abstract</title> <bold>Background:</bold> The relationship between nighttime lights and human health is being discussed in a growing number of studies, with vast majority results supporting an association. However, the impact potential confounding has been insufficiently addressed existing undermining reported associations. <bold>Objective:</bold> This study aimed to assess exposure population outcomes USA careful adjustment confounders. <bold>Methods:</bold> ecological analyzed...

Abstract Target‐site resistance is commonly caused by qualitative changes in insecticide target‐receptors and few studies have implicated quantitative targets resistance. Here we show that to imidacloprid a selected strain of Nilaparvata lugens associated with reduction expression levels the nicotinic acetylcholine receptor (nAChR) subunit Nlα8. Synergism bioassays suggested was conferred, part, target‐site mechanism. Sequencing N. nAChR genes identified no mutations resistance, however,...

Insecticide resistance can arise from a variety of mechanisms, including changes to the target site, but is often associated with substantial fitness costs insects. Here we describe two resistance-associated target-site mutations that have synergistic and compensatory effects combine produce high persistent levels fipronil, an insecticide targeting on γ-aminobytyric acid (GABA) receptors. In Nilaparvata lugens, major pest rice crops in many parts Asia, identified single point mutation...

Abstract The brown planthopper (BPH) Nilaparvata lugens (Stål) is a main pest on rice. It secretes saliva to regulate plant defense responses, when penetrating rice and sucking phloem sap through its stylet. However, the molecular mechanisms of BPH salivary proteins regulating responses remain poorly understood. A N. DNAJ protein (NlDNAJB9) gene was highly expressed in glands, knock down NlDNAJB9 significantly enhanced honeydew excretion fecundity BPH. could induce cell death, overexpression...

Odorant-binding proteins (OBPs) play key roles in the perception of semiochemicals insects. Several OBPs insect olfactory systems have been functionally characterized, and they provide excellent targets for pest control. The functions some that are highly expressed nonsensory organs insects remain unclear. Here, physiological function an OBP (OBP27) was Spodoptera frugiperda studied. OBP27 nested within Plus-C cluster according to phylogenetic analysis. transcription steadily increased...

Cytochrome P450 enzymes metabolize various xenobiotics in insects. Compared to numerous P450s associated with insecticide detoxification and resistance, fewer have been identified bioactivate proinsecticides Here we reported that two P450s, CYP4C62 CYP6BD12, Nilaparvata lugens could chlorpyrifos, an organophosphorus insecticide, into its active ingredient chlorpyrifos-oxon vivo vitro. RNAi knockdown of these genes significantly reduced the sensitivity chlorpyrifos formation N. lugens....

Nilaparvata lugens and Sogatella furcifera are two primary planthoppers on rice throughout Asian countries areas. Neonicotinoid insecticides, such as imidacloprid (IMI), have been extensively used to control IMI resistance consequently occurred with an important mechanism from the over-expression of P450 genes. The induction genes by may increase ability metabolize this insecticide in risk. In study, was compared S. treated nitromethyleneimidazole (NMI), planthopper species lethal dose that...

The pond wolf spider Pardosa pseudoannulata, an important natural predatory enemy of rice planthoppers, is found widely distributed in paddy fields. However, data on the genes involved insecticide action, detoxification, and response are very limited for P. which inhibits development appropriate use selective insecticides to control insect pests rice. We used transcriptome construction from adult cephalothoraxes analyze manually identify enconding metabolic enzymes target receptors related...