ROS-dependent induction of oxidative damage can be used as a trigger initiating genetically determined non-specific protection in plant cells and tissues. Plants are potentially able to withstand various specific (toxic, osmotic) factors abiotic effects, but do not have sufficient or sensitivity form an adequate effective response. In this work, we demonstrate one the possible approaches for successful cold acclimation through formation photosynthetic structures due insertion heterologous...

Unsymmetrical dimethylhydrazine (UDMH) despite its proven high toxicity continues to be used in rocket technology and some other areas of human activity. In this work, the ability plant-bacteria consortia reduce genotoxicity UDMH incomplete oxidation products was investigated. Genotoxicity assessed using a specific lux-biosensor Escherichia coli MG1655 pAlkA-lux sensitive DNA alkylation cell. For microbio-logical biodegradation, Bacillus subtilis KK1112 strain obtained by isolation from soil...

Despite its proven high toxicity, unsymmetrical dimethylhydrazine (UDMH) continues to be used in rocket technology and some other areas of human activity. In this work, the ability plant-bacterial consortia reduce genotoxicity UDMH incomplete oxidation products was investigated. Genotoxicity assessed using a specific lux-biosensor, Escherichia coli MG1655 pAlkA-lux, which emits stronger light when cellular DNA is alkylated. For microbiological biodegradation, Bacillus subtilis KK1112 strain...

Background: Salt stress is a multicomponent phenomenon; it includes many processes that directly or indirectly affect the plant. Attempts have been made to comprehensively consider of salt in plants Triticum aestivum (variety Orenburgskaya 22) and durum Zolotaya). Methods: The study used methods light fluorescence microscopy, immunofluorodetection, expression DNA methyltransferase genes, genes ion transporter superoxide dismutase families, as well biochemical determination total antioxidant...

Various abiotic stresses cause the appearance of reactive oxygen species (ROS) in plant cells, which seriously damage cellular structures. The engineering transgenic plants with higher production ROS-scavenging enzyme cells could protect integrity such a fine intracellular structure as cytoskeleton and each compartment. We analyzed morphological changes root tip caused by application iso-osmotic NaCl Na2SO4 solutions to tomato harboring an introduced superoxide dismutase gene. To study roots...

Understanding the mechanisms of plant salt tolerance as a complex trait is an integral part many studies, results which have been used in breeding process. The aim this study was to compare root response two tomato (Solanum lycopersicum L.) genotypes (breeding line YaLF and cultivar Recordsmen) differing tolerance. Rhizogenesis induced shoots vitro with different concentrations NaCl culture medium. A number morphobiological cytological parameters were evaluated at organ, tissue, cellular...

The endothelium is an additional cell layer, differentiating from the inner epidermis of ovule integument. In tomato (Solanum lycopersicum L.), after fertilization, separates integument and becomes independent tissue developing next to growing embryo sac. absence may proliferate form pseudo-embryo. However, course reorganization into pseudo-embryo in ovules poorly understood. We aimed investigate specific features differentiation role development fertilized unfertilized ovules. plants...

The toxicity of high concentrations sodium chloride creates significant difficulties in realizing the productivity potential wheat. development effective test systems for identification and selection resistant genotypes is an urgent task given global increase soil salinity agricultural land. To identify characteristics plant’s reaction to toxic effect chloride, wheat with different resistance ionic (the Orenburgskaya 10 22 varieties) were used. In model experiments, we used fluorescence,...

We report the primary characterization of a new gene KCNRG mapped at chromosome band 13q14.3. This includes three exons and has two alternatively spliced isoforms that are expressed in normal tissues some tumor cell lines. Protein high homology to tetramerization domain voltage‐gated K + channels. Using patch‐clamp technique we determined suppresses channel activity human prostate line LNCaP. It is known selective blockers channels suppress lymphocyte LNCaP proliferation. suggest candidate...

Duodenase, a serine protease from bovine duodenum mucosa, was located in endoplasmic reticulum, the Golgi secretory granules of epithelial cells and ducts Brunner's glands by A‐gold immunocyto‐chemical method. Duodenase exhibits trypsin‐like chymotrypsin‐like specificities with preference for substrates having lysine at P1 proline P2 positions. The kinetic constants hydrolysis 21 potential duodenase are reported. best were found to be α‐ N ‐tosylglycylprolyllysine 4‐nitroanilide ( k cat / K...

Various stressors lead to an increase in ROS and damage plant tissues. Plants have a powerful antioxidant system (AOS), which allows them neutralize excess ROS. We detected intense fluorescent glow of the cells cap, meristem, elongation zones roots wheat Triticum aestivum (Orenburgskaya 22 variety) durum (Zolotaya variety). An was accompanied by DNA breaks nuclei root cells, release cytochrome c from mitochondria into cytoplasm, translocation phosphatidylserine outer layer plasma membrane...

An image analysis system was used to count and measure acetoorcein-stained mitotic chromosomes of the acidothermophilic unicellular red algae Galdieria maxima, G. partita, sulphuraria Cyanidium caldarium (Cyanidiophyceae). Chromosome numbers fell into two groups: n = 2 for all three species 5(7) C. caldarium. It seems that a separation from is karyologically justified. A chromosome number appears be indicative genus could as marker distinguish this taxon Cyanidium. The smaller in karyotype...

Hypoxia is one of the common abiotic stresses that negatively affects development and productivity agricultural crops. Quercetin used to protect plants from oxidative stress when exposed environmental stressors. O2 deficiency leads impaired morphometric parameters in wheat varieties Orenburgskaya 22 (Triticum aestivum) Zolotaya durum). Cytological analysis revealed various types changes cytoplasm under conditions hypoxia treatment with quercetin. The most critical occur variety during...

Hypoxia is one of the common abiotic stresses that negatively affects development and productivity agricultural crops. Quercetin used to protect plants from oxidative stress when exposed environmental stressors. O2 deficiency leads impaired morphometric parameters in wheat varieties Orenburgskaya 22 (Triticum aestivum L.) Zolotaya durum Desf.). Cytological analysis revealed various types changes cytoplasm under conditions hypoxia treatment with quercetin. The most critical occur variety...

An image analysis system was used to count and measure acetoorcein-stained mitotic chromosomes of the acidothermophilic unicellular red algae Galdieria maxima, G. partita, sulphuraria Cyanidium caldarium (Cyanidiophyceae). Chromosome numbers fell into two groups: n = 2 for all three species 5(7) C. caldarium. It seems that a separation from is karyologically justified. A chromosome number appears be indicative genus could as marker distinguish this taxon Cyanidium. The smaller in karyotype...

The study was devoted to morphological and cytoembryological analysis of disorders in the anther pollen development transgenic tomato plants with a normal abnormal phenotype, which is characterized by impaired generative organs. Various abnormalities structural organization anthers microspores were revealed. Such lead blocking asymmetric cell division and, accordingly, male gametophyte formation. Some non-degenerated accumulate large number storage inclusions, forming sterile mononuclear...

Abstract We investigated how an abiotic factor, i.e. salinity, affects specific intracellular organelles of cells the root cap. focused on: 1) amyloplasts, which are plastids cap that perform important metabolic and sensory functions; 2) mitochondria, protect from ROS damage by changing their ultrastructure. In our work we studied ultrastructural changes these in different areas under NaCl Na 2 SO 4 impact. showed amyloplasts (statoliths) mitochondria columella peripheral zone change...

Determining salt tolerance potential in wheat is one of the most important problems breeding practice for areas with primary and secondary salinity. Presence large saline soils results inhibition growth, development stability obtaining high yields agricultural plants. Therefore, there a need comprehensive studying improving diagnostic methods during early growth stages. Different genotypes Triticum aestivum Host. durum Desf. were used to identify markers. Both morphometric some biochemical...