A5082942674- Plant Molecular Biology Research
- Microtubule and mitosis dynamics
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- DNA Repair Mechanisms
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Plant tissue culture and regeneration
- DNA and Nucleic Acid Chemistry
- Polysaccharides and Plant Cell Walls
- Nanoparticles: synthesis and applications
- Plant Stress Responses and Tolerance
- Agricultural Productivity and Crop Improvement
- Plant Physiology and Cultivation Studies
- Bacterial Genetics and Biotechnology
- Antioxidant Activity and Oxidative Stress
- Enzyme Structure and Function
- Neurological diseases and metabolism
- Adipose Tissue and Metabolism
- Autophagy in Disease and Therapy
- Plant Genetic and Mutation Studies
- Radioactive element chemistry and processing
- Lipid metabolism and biosynthesis
- Graphene and Nanomaterials Applications
Lomonosov Moscow State University
2015-2024
Moscow State University
2002-2024
All-Russian Scientific Research Institute of Food Biotechnology
2017-2023
Russian State Agrarian University - Moscow Timiryazev Agricultural Academy
2020-2021
Joint Institute for Nuclear Research
2018-2020
Russian Academy of Sciences
2003-2020
Institute of Molecular Genetics
2003-2016
Kurchatov Institute
2003-2016
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2005-2009
National Institutes of Health
2005-2009
Mutations in the human dynamin-related protein Drp1 cause mitochondria to form perinuclear clusters. We show here that these mitochondrial clusters consist of highly interconnected tubules. The increased connectivity between indicates balance division and fusion is shifted toward fusion. Such a shift consistent with block division. Immunofluorescence subcellular fractionation endogenous localized mitochondria, which also role A direct suggested by time-lapse photography transfected cells,...
Mitochondria exist as a dynamic tubular network with projections that move, break, and reseal in response to local environmental changes. We present evidence human dynamin-related protein (Drp1) is specifically required establish this morphology. Drp1 GTPase domain structure similar of other dynamin family members. To identify the function Drp1, we transiently transfected cells mutant Drp1. A mutation caused profound alterations mitochondrial The normally wild-type were retracted into large...
Lipid droplets (LDs) are cytoplasmic organelles that store neutral lipids for use as an energy supply in times of nutrient deprivation and membrane assembly. Misregulation LD function leads to many human diseases, including lipodystrophy, obesity lipid storage disorders. A number proteins have been shown localize the surface droplets, lipases such adipose triglyceride lipase (ATGL) PAT-domain ADRP (adipophilin) TIP47, but mechanism by which they targeted LDs is not known. Here we demonstrate...
Data showing that an apoptotic reaction (the exit into the cytoplasm and nucleolytic internucleosomal degradation of chromosomal DNA, compaction fragmentation chromatin, cellular shrinkage, cytoplasmic blebbing) developed in a subline HeLa-S3 cells upon nonpermissive poliovirus infection with either guanidine-sensitive presence guanidine, guanidine-dependent mutant absence or certain temperature-sensitive mutants at restrictive temperature are presented. Essentially, no occurred permissive...
Dynamin is a 100-kDa GTPase that assembles into multimeric spirals at the necks of budding clathrin-coated vesicles. We describe three different intramolecular binding interactions may account for process dynamin self-assembly. The first interaction dimerization 100-amino acid segment in C-terminal half dynamin. call this assembly domain, because it appears to be critical multimerization. second occurs between domain and N-terminal domain. strength controlled by nucleotide-bound state as...
ROS-dependent induction of oxidative damage can be used as a trigger initiating genetically determined non-specific protection in plant cells and tissues. Plants are potentially able to withstand various specific (toxic, osmotic) factors abiotic effects, but do not have sufficient or sensitivity form an adequate effective response. In this work, we demonstrate one the possible approaches for successful cold acclimation through formation photosynthetic structures due insertion heterologous...
Abstract We analyzed the distribution and orientation of transitory microtubule structures, converging centers, during interphase mitosis in endosperm higher plant Haemanthus. In pointed tips centers are associated with nuclear envelope. Their gradually reverses prophase, tend to point away from nucleus. From prometaphase through early telophase, present predominantly cytoplasm at polar region. They either “free” or chromosomes bundles. late again reconstructed envelope and, additionally,...
DNA double stranded breaks (DSBs) are the most serious type of lesions introduced into chromatin by ionizing radiation. During DSB repair, cells recruit different proteins to damaged sites in a manner dependent on local structure, location nucleus, and repair pathway entered. 53BP1 is one important players participating decision cell. Although many molecular biology details have been investigated, architecture foci its development during post-irradiation time, especially period protein...
Entosis, or cell death by invading another cell, is typical for tumor epithelial cells. The formation of cell-in-cell structures extensively studied in suspension cultures, but remains poorly understood substrate-dependent Here, we used electron, confocal and time-lapse microscopy combination with pharmacological inhibition intracellular components to study the kinetics entosis using two human A431 MCF7. In total, identified characterized five consecutive stages entosis, which were common...
Microtubule-based motor proteins assemble and reorganize acentrosomal mitotic meiotic spindles in animal cells. The functions of plant are unknown. cellulosic cell wall relative small size most cells precludes accurate detection the spatial distribution motors mitosis. Large absence a Haemanthus endosperm make these ideally suited for studies during division. Immunolocalization kinesin-like calmodulin-binding protein (KCBP) revealed its distribution. KCBP appears first association with...
We analyzed transformation of the interphase microtubular cytoskeleton into prophase spindle and followed pattern axis determination. Microtubules in endosperm higher plant Haemanthus (Scadoxus) were stained by immunogold silver-enhanced methods. Basic structural units involved morphogenesis "microtubule converging centers." emphasized importance relative independence chromosomal cycles, influence these cycles on progress mitosis. Cells with moderately desynchronized functional, but extreme...
Engineered nanoparticles (ENPs) are now being used in many sectors of industry; however, the impact ENPs on environment still requires further study, since their use, recycling, and accidental spill can result accumulation atmosphere, soil, water. Plants an integral part ecosystems; hence interaction with is inevitable. It important to understand consequences this assess its potential effects. The present research focused studying effects industrial material Taunit, containing multi-walled...
Various abiotic stresses cause the appearance of reactive oxygen species (ROS) in plant cells, which seriously damage cellular structures. The engineering transgenic plants with higher production ROS-scavenging enzyme cells could protect integrity such a fine intracellular structure as cytoskeleton and each compartment. We analyzed morphological changes root tip caused by application iso-osmotic NaCl Na2SO4 solutions to tomato harboring an introduced superoxide dismutase gene. To study roots...
Uridine phosphorylase from E. coli (Upase) has been crystallized using vapor diffusion technique in a new monoclinic crystal form. The structure was determined by the molecular replacement method at 2.5 Å resolution. coordinates of trigonal form were used as starting model and refinement program XPLOR led to R‐factor 18.6%. amino acid fold protein found be same that crystals. positions flexible regions refined. conclusion about involvement active site is good agreement with results...
The endothelium is an additional cell layer, differentiating from the inner epidermis of ovule integument. In tomato (Solanum lycopersicum L.), after fertilization, separates integument and becomes independent tissue developing next to growing embryo sac. absence may proliferate form pseudo-embryo. However, course reorganization into pseudo-embryo in ovules poorly understood. We aimed investigate specific features differentiation role development fertilized unfertilized ovules. plants...