A5091516433- Advanced Fluorescence Microscopy Techniques
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Advanced biosensing and bioanalysis techniques
- DNA Repair Mechanisms
- Genomic variations and chromosomal abnormalities
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Near-Field Optical Microscopy
- Advanced Biosensing Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Molecular Biology Techniques and Applications
- RNA and protein synthesis mechanisms
- Radiation Therapy and Dosimetry
- Microfluidic and Bio-sensing Technologies
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- Dialysis and Renal Disease Management
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Single-cell and spatial transcriptomics
- Monoclonal and Polyclonal Antibodies Research
- Carcinogens and Genotoxicity Assessment
- CRISPR and Genetic Engineering
- Microfluidic and Capillary Electrophoresis Applications
Ben-Gurion University of the Negev
2002-2025
Kirchhoff (Germany)
2015-2024
Heidelberg University
2015-2024
Sheba Medical Center
2024
Assuta Medical Center
2022-2023
Université Paris-Saclay
2023
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2023
CEA Paris-Saclay
2023
Laboratoire Interactions, Dynamiques et Lasers
2023
Institut Lumière Matière
2023
Granzyme (gzm) A and gzmB have been implicated in Fas-independent nucleolytic cytolytic processes exerted by cytotoxic T (Tc) cells, but the underlying mechanism(s) remains unclear. In this study, we compare potential of Tc natural killer (NK) cells mice deficient both gzmA B (gzmA×B−/−) with those from single knockout (−/−), or perforin (−/−) to induce nuclear damage lysis target cells. With exception perforin−/−, all vitro– ex vivo–derived NK cell populations mutant strains induced...
Background Circulating cell-free DNA (CFD) levels may be elevated in trauma, stroke, sepsis, pre-eclampsia and cancer. Owing to the complex expensive methodology, detection of CFD has hitherto been confined research laboratories. This study presents a simple, inexpensive accurate test for CFD. Methods Using commercial fluorescent SYBR ® Gold stain, biological fluids were directly assayed without prior extraction amplification. Stain was added sample 96-well plates (final stain dilution:...
Arrays promise to advance biology through parallel screening for binding partners. We show the combinatorial in situ synthesis of 40,000 peptide spots per square centimeter on a microchip. Our variant Merrifield immobilizes activated amino acids as monomers within particles, which are successively attracted by electric fields generated each pixel electrode chip. With all different addressed, particles melted at once initiate coupling. Repetitive coupling cycles should allow translation whole...
Special delivery: The "freezing" of activated amino acid derivatives within solid particles enables a laser printer to deliver these "postal packages" defined locations on support with high resolution. Subsequent parallel coupling is initiated simply by melting whole layer 20 different (see schematic representation; Fmoc=9-fluorenylmethoxycarbonyl).
The Her2/neu tyrosine kinase receptor is a member of the epidermal growth factor family. It plays an important role in tumour genesis certain types breast cancer and its overexpression correlates with distinct diagnostic therapeutic decisions. Nevertheless, it still under intense investigation to improve outcome therapy control. In this content, we applied spectral precision distance/position determination microscopy, technique based on general principles localization microscopy order study...
Summary We present a novel technique of far‐field localization nanoscopy combining spectral precision distance microscopy with widely used fluorochromes like the Green Fluorescent Protein (GFP) derivatives eGFP, EmGFP, Yellow (YFP) and eYFP, synthetic dyes Alexa 488 568, as well fluoresceine derivates. Spectral allows surpassing conventional resolution limits in fluorescence by precise object after optical isolation single signals time. Based on principles this technique, our nanoscopic...
Topological analysis of the three‐dimensional (3D) chromatin nanostructure and its function in intact cell nuclei implies use high resolution far field light microscopy, e.g. confocal laser scanning microscopy (CLSM). However, experimental evidence indicates that, practice, under biologically relevant conditions, spatial CLSM is limited to about 300 nm lateral direction 700 axial direction. To overcome this shortcoming, a recently developed microscopical approach, spectral precision distance...
Ionizing radiation (IR)-induced damage confers functional and conformational changes to nuclear chromatin associated with DNA single double strand breaks.
It has been well established that the architecture of chromatin in cell nuclei is not random but functionally correlated. Chromatin damage caused by ionizing radiation raises complex repair machineries. This accompanied local rearrangements and structural changes which may for instance improve accessibility damaged sites protein complexes. Using stably transfected HeLa cells expressing either green fluorescent (GFP) labelled histone H2B or yellow (YFP) H2A, we investigated positioning...
In genome analysis,
Understanding molecular interactions and regulatory mechanisms in tumor initiation, progression, treatment response are key requirements towards advanced cancer diagnosis novel procedures personalized medicine. Beyond decoding the gene expression, malfunctioning cancer-related epigenetic pathways, investigations of spatial receptor arrangements membranes genome organization cell nuclei, on nano-scale, contribute to elucidating complex cells tissues. By these means, correlation between...
From the very beginnings of radiotherapy, a crucial question persists with how to target radiation effectiveness into tumor while preserving surrounding tissues as undamaged possible. One promising approach is selectively pre-sensitize cells by metallic nanoparticles. However, though “physics” behind nanoparticle-mediated radio-interaction has been well elaborated, practical applications in medicine remain challenging and often disappointing because limited knowledge on biological mechanisms...
Finding out how cells prepare for fate change during differentiation commitment was our task. To address whether the constitutive pericentromere-associated domains (PADs) may be involved, we used a model system with known transcriptome data, MCF-7 breast cancer treated ErbB3 ligand heregulin (HRG), which induces and is in therapy of cancer. PAD-repressive heterochromatin (H3K9me3), centromere-associated-protein-specific, active euchromatin (H3K4me3) antibodies, real-time PCR, acridine orange...
The Yanomamo Indians live in the tropical equatorial rain forests of northern Brazil and southern Venezuela. They essentially eat a salt free diet. As such, they have been demonstrated to minimal 24 h urine sodium excretion, markedly elevated serum renin aldosterone levels, low normal blood pressure (BP) throughout their lives. We present an unusual case 75-year-old woman, with chronic kidney disease (CKD), stage 1 hypertension (HTN), partial colectomy who maintained strict diet while using...
Abstract Array‐based comparative genomic hybridization (aCGH) allows the identification of DNA sequence copy number changes at high resolution by co‐hybridizing differentially labelled test and control DNAs to a micro‐array clones. The present study has analysed series 23 formalin‐fixed, paraffin wax‐embedded tissue samples Barrett's adenocarcinoma (BCA, n = 18) non‐neoplastic squamous oesophageal ( 2) gastric cardia mucosa 3) aCGH. micro‐arrays used contained 287 targets covering oncogenes,...