A5050954711- Influenza Virus Research Studies
- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Extracellular vesicles in disease
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- HIV Research and Treatment
- Cytomegalovirus and herpesvirus research
- Genomics and Chromatin Dynamics
- Lymphoma Diagnosis and Treatment
- Malaria Research and Control
- Immunotherapy and Immune Responses
- Neuroinflammation and Neurodegeneration Mechanisms
- Bacteriophages and microbial interactions
- Advanced Electron Microscopy Techniques and Applications
- Virus-based gene therapy research
- Tryptophan and brain disorders
- Cellular transport and secretion
- Nanoplatforms for cancer theranostics
- Lipid Membrane Structure and Behavior
Instituto Gulbenkian de Ciência
2016-2025
Calouste Gulbenkian Foundation
2022-2023
Influenza A virus has an eight-partite RNA genome that during viral assembly forms a complex containing one copy of each RNA. Genome is selective process driven by RNA-RNA interactions and hypothesized to lead discrete punctate structures scattered through the cytosol. Here, we show contrary accepted view, formation these precedes among distinct ribonucleoproteins (vRNPs), as they assemble in cells expressing only vRNP type. We demonstrate inclusions display characteristics liquid...
ABSTRACT The centrosome is an important microtubule-organising centre (MTOC) in animal cells. It consists of two barrel-shaped structures, the centrioles, surrounded by pericentriolar material (PCM), which nucleates microtubules. Centrosomes can form close to existing structure (canonical duplication) or de novo. How centrosomes novo not known. master driver biogenesis, PLK4, critical for recruitment several centriole components. Here, we investigate beginning taking advantage Xenopus egg...
Influenza A virus assembly is an unclear process, whereby individual virion components form infectious particle. The segmented nature of the influenza genome imposes a problem to because it requires packaging eight distinct RNA particles (vRNPs). It also allows mixing from parental strains, events associated with pandemic outbreaks. important public health understand how genomes assemble, process that dependent on transport sites. Previously, has been shown vRNPs are carried by recycling...
Whether jaundice, a common presentation of Plasmodium ( P .) falciparum malaria (1-3) arising from the accumulation circulating bilirubin, represents an adaptive or maladaptive response to spp. infection is not understood (1-3). We found that asymptomatic P. was associated with >10-fold higher ratio unconjugated bilirubin over parasite burden, compared symptomatic malaria. Genetic suppression synthesis by biliverdin reductase A (BVRA) (4) increased virulence and mortality in mice....
It is now established that many viruses threaten public health establish condensates via phase transitions to complete their lifecycles, and knowledge on such processes may offer new strategies for antiviral therapy. In the case of influenza A virus (IAV), liquid known as viral inclusions, concentrate 8 distinct ribonucleoproteins (vRNPs) form IAV genome are viewed sites dedicated assembly 8-partite genomic complex. Despite not being delimited by host membranes, inclusions accumulate...
Acellular bronchoalveolar lavage (BAL) proteomics can partially separate lung cancer from non-lung patients based on principal component analysis and multivariate analysis. Furthermore, the variance in data sets is correlated mainly with status and, to a lesser extent, smoking gender. Despite these advances BAL small large extracellular vehicles (EVs) proteomes reveal aberrant protein expression paracrine signaling mechanisms initiation progression. We consequently present case-control study...
The role of extracellular vesicles (EVs) proteome in diffuse large B-cell lymphoma (DLBCL) pathology, subclassification, and patient screening is unexplored. We analyzed by state-of-the-art mass spectrometry the whole cell secreted proteomes different molecular subtypes DLBCL, germinal center B (GCB subtype), activated (ABC subtype). After quality control assessment, we compared whole-cell EVs two cell-of-origin (COO) categories, GCB ABC subtypes, resulting 288/1115 significantly...
In many cases protein assemblies are stabilized by covalent bonds, one example of which is the formation intra- or intermolecular ε-(γ-glutamyl)lysil cross-links catalyzed transglutaminases (TGases). Because potential for unwanted cross-linking reactions, activities TGases have been shown to be tightly controlled. Bacterial endospores highly resilient cells in part because they surrounded a complex coat. Proteins coat that surrounds Bacillus subtilis crosslinked TGase (Tgl). Unlike other...
Summary statement PLK4 binds to microtubules and self assembles into supramolecular assemblies that recruit tubulin trigger de novo MTOC formation in Xenopus laevis extracts. Abstract The centrosome is an important microtubule-organizing center (MTOCs) animal cells it consists of two barrel-shaped structures (centrioles), surrounded by the pericentriolar material (PCM), which nucleates microtubules. PCM components form condensates, concentrate microtubule nucleators. Centrosomes can close...
"Using correlative light and electron microscopy to understand influenza A viral assembly." Ultrastructural Pathology, 41(1), pp. 80–81
ABSTRACT Influenza A virus has an eight-partite RNA genome that during viral assembly forms a supramolecular complex containing one copy of each RNA. Genome is selective process driven by RNA-RNA interactions and thought to lead discrete punctate structures scattered through the cytosol. Here, we show contrary accepted view, formation these not dependent on among distinct ribonucleoproteins (vRNPs), as they assemble in cells expressing only vRNP type. We demonstrate inclusions display...
Centrioles are structurally conserved organelles, composing both centrosomes and cilia. In animal cycling cells, centrioles often form through a highly characterized process termed canonical duplication. However, large diversity of eukaryotes de novo uncharacterized pathways. This unexplored is key to understanding centriole assembly mechanisms how they evolved assist specific cellular functions. Here, combining electron microscopy tomography, we show that during spermatogenesis the moss...
ABSTRACT It is now established that many viruses threaten public health establish condensates via phase transitions to complete their lifecycles, and knowledge on such processes may offer new strategies for antiviral therapy. In the case of influenza A virus (IAV), liquid known as viral inclusions, concentrate 8 distinct ribonucleoproteins (vRNPs) form IAV genome are viewed sites dedicated assembly 8-partite genomic complex. Despite not being delimited by host membranes, inclusions...
Centrioles are structurally conserved organelles, composing both centrosomes and cilia. In animal cycling cells, centrioles often form through a highly characterized process termed canonical duplication. However, large diversity of eukaryotes de novo uncharacterized pathways. This unexplored is key to understanding centriole assembly mechanisms how they evolved assist specific cellular functions. Here, combining electron microscopy tomography, we show that during spermatogenesis the moss...