A5048668591- Immune responses and vaccinations
- Mosquito-borne diseases and control
- Neonatal Respiratory Health Research
- Immune cells in cancer
- Malaria Research and Control
- Adenosine and Purinergic Signaling
- Acute Kidney Injury Research
- Epigenetics and DNA Methylation
- COVID-19 epidemiological studies
- Adipokines, Inflammation, and Metabolic Diseases
- Obstructive Sleep Apnea Research
- Heat shock proteins research
- Viral Infections and Vectors
- COVID-19 Impact on Reproduction
- SARS-CoV-2 and COVID-19 Research
- Vaccine Coverage and Hesitancy
- HIV Research and Treatment
- Effects of Radiation Exposure
- Sulfur Compounds in Biology
- Inhalation and Respiratory Drug Delivery
- Asthma and respiratory diseases
- PARP inhibition in cancer therapy
- Diabetes and associated disorders
- Complement system in diseases
- Single-cell and spatial transcriptomics
Jena University Hospital
2017-2025
Friedrich Schiller University Jena
2021-2025
Instituto Gulbenkian de Ciência
2021-2023
University College London
2017-2018
Bury College
2017-2018
Universities of Giessen and Marburg Lung Center
2017
German Center for Lung Research
2017
Significance During infection, extracellular “labile” heme, released from damaged red blood or parenchymal cells, acts as prototypical alarmin stimulating myeloid cells. A characteristic hallmark of cell activation is the development trained immunity, specified long-lasting adaptations based on transcriptional and epigenetic modifications. In vivo, this maintained by rerouting hematopoiesis. We found that heme a previously unrecognized immunity inducer promoting resistance to bacterial...
Whether jaundice, a common presentation of Plasmodium ( P .) falciparum malaria (1-3) arising from the accumulation circulating bilirubin, represents an adaptive or maladaptive response to spp. infection is not understood (1-3). We found that asymptomatic P. was associated with >10-fold higher ratio unconjugated bilirubin over parasite burden, compared symptomatic malaria. Genetic suppression synthesis by biliverdin reductase A (BVRA) (4) increased virulence and mortality in mice....
Multicellular organisms rely on inter-organ communication networks to maintain vital parameters within a dynamic physiological range. Macrophages are central this homeostatic control system, sensing deviations of those and responding accordingly support tissue function organismal homeostasis. Here we demonstrate that dysregulation iron metabolism in parenchyma cells, imposed by the deletion ferritin H chain, is sensed monocyte-derived macrophages. In response, macrophages derived from...
Histone methyltransferase G9a, also known as Euchromatic Lysine Methyltransferase 2 (EHMT2), mediates H3K9 methylation which is associated with transcriptional repression. It possesses immunomodulatory effects and overexpressed in multiple types of cancer. In this study, we investigated the role G9a induction trained immunity, a de facto innate immune memory, its non-muscle-invasive bladder cancer (NMIBC) patients treated intravesical Bacillus Calmette-Guérin (BCG).EHMT2 expression was...
Hydrogen sulfide (H 2 S) has recently been recognized as a novel gaseous transmitter with several anti-inflammatory properties. The role of host- derived H S in infections by Pseudomonas aeruginosa was investigated clinical and mouse models. concentrations survival assessed septic patients lung infection. Animal experiments using model severe systemic multidrug-resistant P . infection were performed mice constitutive knock-out cystathionine-γ lyase ( Cse) gene Cse -/- ) wild-type...
To characterize the temporal pattern of a panel blood and urinary biomarkers in an animal model fecal peritonitis recovery.Prospective observational study.University research laboratory.Male Wistar rats.A fluid-resuscitated, long-term (3 d) rat sepsis (fecal peritonitis) recovery was used to understand association relation systemic hemodynamics, inflammation, renal function. At predefined time points (3, 6, 12, 24, 48, 72 hr), animals (≥ 6 per group) underwent echocardiography, urine...
Sepsis is a major clinical problem associated with significant organ dysfunction and high mortality. The ATP-sensitive P2X7 receptor activates the NLRP3 inflammasome key component of innate immune system. We used fluid-resuscitated rat model fecal peritonitis acute kidney injury (AKI) to investigate contribution this purinergic renal in sepsis. Six 24 h time-points were chosen represent early established sepsis, respectively. A selective antagonist (A-438079) dissolved dimethyl sulfoxide...
Background Legionella pneumophila (L. pneumophila) is a causative agent of severe pneumonia. It highly adapted to intracellular replication and manipulates host cell functions like vesicle trafficking mRNA translation its own advantage. However, it still unknown what extent microRNAs (miRNAs) are involved in the Legionella-host interaction. Methods WT MyD88-/- murine bone marrow-derived macrophages (BMM) were infected with L. pneumophila, transcriptome was analyzed by high throughput qPCR...
ABSTRACT Trained immunity defines long-lasting adaptive responses of innate immunity, mediated by transcriptional and epigenetic modifications myeloid cells. Here, we report that labile heme, an alarmin released extracellularily upon tissue damage hemolysis, induces trained in human murine primary cells mice. Heme-trainning monocytes is associated with profiles overlap to some extent those seen β-glucan-training, the prototype agonist immunity. In sharp contrast β-glucan training however,...
Acute pancreatitis is a severe systemic disease triggered by sterile inflammation and initial local tissue damage of the pancreas. Immune cells infiltrating into pancreas are main mediators acute pathogenesis. In addition to their antimicrobial potency, macrolides possess anti-inflammatory immunomodulatory properties which routinely used in patients with chronic airway infections might also beneficial treatment lung injury. We here tested hypothesis that macrolide antibiotic azithromycin can...
Bulk RNA sequencing of Plasmodium spp., the causative parasite malaria, fails to discriminate developmental-stage-specific gene regulation. Here, we provide a protocol that uses single-cell FACS-sorted Plasmodium-chabaudi-chabaudi-AS-infected red blood cells (iRBCs) characterize modulation expression during malaria stage. We describe steps for infecting mice, monitoring disease progression, preparing iRBCs, and iRBCs. then detail procedures analyzing scRNA-seq data. For complete details on...
SUMMARY Hypoglycemia is a clinical hallmark of severe malaria, the often-lethal presentation Plasmodium falciparum infection humans. Here we report that mice reduce blood glucose levels in response to via coordinated whereby labile heme, an alarmin produced hemolysis, induces anorexia and represses hepatic production (HGP). While protective against unfettered immune-mediated inflammation, organ damage anemia, when sustained over time heme-driven repression HGP can progress towards...