A5034837078- COVID-19 Clinical Research Studies
- Sepsis Diagnosis and Treatment
- Blood disorders and treatments
- Inflammation biomarkers and pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Pneumonia and Respiratory Infections
- Immune responses and vaccinations
- Neonatal and Maternal Infections
- Biosensors and Analytical Detection
- Pneumocystis jirovecii pneumonia detection and treatment
- Thermal Regulation in Medicine
- Nosocomial Infections in ICU
- Immune cells in cancer
- Tuberculosis Research and Epidemiology
- Long-Term Effects of COVID-19
- Respiratory viral infections research
- S100 Proteins and Annexins
- Extracellular vesicles in disease
- Diabetes and associated disorders
- Thyroid Disorders and Treatments
- Immunodeficiency and Autoimmune Disorders
- Antimicrobial Resistance in Staphylococcus
National and Kapodistrian University of Athens
2012-2024
Athens Medical Center
2024
University of Lausanne
2021-2022
University General Hospital Attikon
2012
Abstract Background The SARS-CoV-2 pandemic is currently leading to increasing numbers of COVID-19 patients all over the world. Clinical presentations range from asymptomatic, mild respiratory tract infection, severe cases with acute distress syndrome, failure, and death. Reports on a dysregulated immune system in call for better characterization understanding changes system. Methods In order dissect COVID-19-driven host responses, we performed RNA-seq whole blood cell transcriptomes...
The state of immune activation may guide targeted immunotherapy in sepsis. In a double-blind, double-dummy randomized clinical study, 240 patients with sepsis due to lung infection, bacteremia, or acute cholangitis were subjected measurements serum ferritin and HLA-DR/CD14. Patients macrophage activation-like syndrome (MALS) immunoparalysis treatment anakinra recombinant interferon-gamma placebo. Twenty-eight-day mortality was the primary endpoint; classification secondary endpoint. Using...
The pandemic 2019 novel coronavirus disease (COVID-19) shares certain clinical characteristics with other acute viral infections. We studied the whole-blood transcriptomic host response to severe respiratory syndrome 2 (SARS-CoV-2) using RNAseq from 24 healthy controls and 62 prospectively enrolled patients COVID-19. then compared these data non-COVID-19 infections, curated 23 independent studies profiling 1,855 blood samples covering six viruses (influenza, syncytial virus (RSV), human...
Abstract Background Sepsis guidelines suggest immediate start of resuscitation for patients with quick Sequential Organ Failure Assessment (qSOFA) 2 or 3. However, the interpretation qSOFA 1 remains controversial. We investigated whether measurements soluble urokinase plasminogen activator receptor (suPAR) may improve risk detection when is 1. Methods The study had two parts. At first part, combination suPAR was analyzed in a prospective cohort early detection. second double-blind,...
Introduction Sepsis is a major cause of death among hospitalised patients. Accumulating evidence suggests that immune response during sepsis cascade lies within spectrum dysregulated host responses. On the one side there are patients whose characterised by fulminant hyperinflammation or macrophage activation-like syndrome (MALS), and on other immunoparalysis. A sizeable group situated between two extremes. Recognising endotype very important in order to choose appropriate immunotherapeutic...
Choice of empirically prescribed antimicrobials for sepsis management depends on epidemiological factors. The epidemiology in Greece was studied two large-periods.
Trained immunity refers to the ability of innate immune system exposed a first challenge provide an enhanced response secondary homologous or heterologous challenge. We reported that training induced with β-glucan one week before infection confers protection against broad-spectrum lethal bacterial infections. Whether this persists over time is unknown. To tackle question, we analyzed status and Listeria monocytogenes (L. monocytogenes) mice trained 9 weeks analysis. The induction increased...
Abstract Predicting the severity of COVID-19 remains an unmet medical need. Our objective was to develop a blood-based host-gene-expression classifier for viral infections and validate it in independent data, including COVID-19. We developed logistic regression-based validated multiple infection settings used training data (N = 705) from 21 retrospective transcriptomic clinical studies influenza other illnesses looking at preselected panel host immune response messenger RNAs. selected 6 RNAs...
Failure of circulating monocytes for adequate cytokine production is a trait sepsis-induced immunosuppression; however, its duration and association with final outcome are poorly understood.We conducted substudy large randomised clinical trial. Peripheral blood mononuclear cells (PBMCs) were isolated within the first 24 h from onset systemic inflammatory response syndrome in 95 patients microbiologically confirmed or clinically suspected gram-negative infections. Isolation was repeated on...
Abstract Context Lymphopenia is a key feature of immune dysfunction in patients with bacterial sepsis and coronavirus disease 2019 (COVID-19) associated poor clinical outcomes, but the cause largely unknown. Severely ill may present thyroid function abnormalities, so-called nonthyroidal illness syndrome, several studies have linked thyrotropin (thyroid stimulating hormone, TSH) hormones thyroxine (T4) 3,5,3′-triiodothyronine (T3) to homeostatic regulation lymphocyte populations. Objective...
SUMMARY The SARS-CoV-2 pandemic is currently leading to increasing numbers of COVID-19 patients all over the world. Clinical presentations range from asymptomatic, mild respiratory tract infection, severe cases with acute distress syndrome, failure, and death. Reports on a dysregulated immune system in calls for better characterization understanding changes system. Here, we profiled whole blood transcriptomes 39 10 control donors enabling data-driven stratification based molecular phenotype....
Hydrogen sulfide (H 2 S) has recently been recognized as a novel gaseous transmitter with several anti-inflammatory properties. The role of host- derived H S in infections by Pseudomonas aeruginosa was investigated clinical and mouse models. concentrations survival assessed septic patients lung infection. Animal experiments using model severe systemic multidrug-resistant P . infection were performed mice constitutive knock-out cystathionine-γ lyase ( Cse) gene Cse -/- ) wild-type...
We used unsupervised immunophenotyping of blood leukocytes and measured cytokine production by innate immune cell exposed to LPS R848. show that COVID-19 induces a rapid, transient upregulation myeloid-derived suppressor cells (MDSCs) accompanied sustained (up 3 months) hyporesponsiveness dendritic monocytes. Blood MDSCs may represent biomarkers targets for intervention strategies in patients.
Introduction: The dysregulated immune response in sepsis is highly variable, ranging from hyperinflammation to immunoparalysis. Obesity associated with the release of inflammatory mediators adipose tissue, known as adipocytokines, causing a chronic state. Perhaps counterintuitively, obesity also lower mortality patients. We investigated association between obesity, circulating adipocytokine concentrations, dysregulation, and outcome Methods In this secondary analysis prospective study,...
TREM-1 (triggering receptor expressed on myeloid cells), a neutrophils and monocytes, is upregulated in sepsis seems to tune the inflammatory response. We explored expression of at gene level cell membranes monocytes association with clinical outcome. Peripheral venous blood was sampled from 75 septic patients (39 sepsis, 25 severe 11 shock) days 1, 3 7. measured by flow cytometry; circulating mononuclear cells assessed real-time PCR. sTREM-1 serum an enzyme immunoassay. Although surface...
Abstract Different immune phenotypes characterize sepsis patients, including hyperinflammation and/or immunosuppression, but the biological mechanisms driving this heterogeneity remain largely unknown. We used single-cell RNA sequencing to profile circulating leukocytes of healthy controls and patients classified as either hyperinflammatory (macrophage activation-like syndrome [MALS]), paralysis , or unclassified (when criteria for neither these two subgroups were applicable). Pronounced...
Mucormycosis has emerged as a group of severe infections mainly in immunocompromised patients. We analysed the epidemiology mucormycosis Greece multicentre, nationwide prospective survey patients all ages, during 2005-2022. A total 108 cases were recorded. The annual incidence declined after 2009 and appeared stable thereafter, at 0.54 cases/million population. most common forms rhinocerebral (51.8%), cutaneous (32.4%), pulmonary (11.1%). Main underlying conditions haematologic...
Abstract Background The accuracy of a new optical biosensor (OB) point-of-care device for the detection severe infections is studied. Methods OB emits different wavelengths and outputs information associated with heart rate, pulse oximetry, levels nitric oxide kidney function. At first phase, recordings were done every two hours three consecutive days after hospital admission in 142 patients at high-risk sepsis by placing on forefinger. second single 54 symptoms viral infection; 38 diagnosed...