A5057075908- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- interferon and immune responses
- Immune responses and vaccinations
- Viral-associated cancers and disorders
- Single-cell and spatial transcriptomics
- SARS-CoV-2 detection and testing
- Immune cells in cancer
- Lymphoma Diagnosis and Treatment
- Clostridium difficile and Clostridium perfringens research
- Viral Infections and Vectors
- Pharmacological Effects of Natural Compounds
- RNA Interference and Gene Delivery
- Liver Disease Diagnosis and Treatment
- Animal Disease Management and Epidemiology
- Reproductive System and Pregnancy
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Long-Term Effects of COVID-19
- Respiratory viral infections research
- Cancer-related molecular mechanisms research
- Polyomavirus and related diseases
- Hepatitis B Virus Studies
Stanford University
2017-2024
Singapore Immunology Network
2017
The pandemic 2019 novel coronavirus disease (COVID-19) shares certain clinical characteristics with other acute viral infections. We studied the whole-blood transcriptomic host response to severe respiratory syndrome 2 (SARS-CoV-2) using RNAseq from 24 healthy controls and 62 prospectively enrolled patients COVID-19. then compared these data non-COVID-19 infections, curated 23 independent studies profiling 1,855 blood samples covering six viruses (influenza, syncytial virus (RSV), human...
Viral infections induce a conserved host response distinct from bacterial infections. We hypothesized that the is associated with disease severity and between patients different outcomes. To test this, we integrated 4,780 blood transcriptome profiles aged 0 to 90 years infected one of 16 viruses, including SARS-CoV-2, Ebola, chikungunya, influenza, across 34 cohorts 18 countries, single-cell RNA sequencing 702,970 immune cells 289 samples three cohorts. Severe viral infection was increased...
Abstract Predicting the severity of COVID-19 remains an unmet medical need. Our objective was to develop a blood-based host-gene-expression classifier for viral infections and validate it in independent data, including COVID-19. We developed logistic regression-based validated multiple infection settings used training data (N = 705) from 21 retrospective transcriptomic clinical studies influenza other illnesses looking at preselected panel host immune response messenger RNAs. selected 6 RNAs...
Older age, being male, obesity, smoking, and comorbidities (e.g., diabetes, asthma) are associated with the increased risk for severe infections. We hypothesized that there is a conserved common immune dysregulation across these factors. integrated single cell bulk transcriptomic data proteomic from 12,026 blood samples 68 cohorts to test this hypothesis. found our previously described 42-gene Severe-or-Mild (SoM) signature, each of factors prior infection. Furthermore, we signature...
Epstein-Barr Virus (EBV) is a ubiquitous virus linked to variety of lymphoid and epithelial malignancies. In solid organ hematopoietic stem cell transplant recipients, EBV causally associated with posttransplant lymphoproliferative disorder (PTLD), group heterogeneous diseases. EBV+ B lymphomas that develop in the context PTLD are generally attributed immunosuppression required promote graft survival, but little known regarding role genome diversity development malignancy. We deep-sequenced...
Alloimmune responses in acute rejection are complex, involving multiple interacting cell types and pathways. Deep profiling of these has been limited by technology that lacks the capacity to resolve this high dimensionality. Single-cell mass cytometry is used characterize alloimmune response early rejection, measuring 37 parameters simultaneously, across time points two models: a murine cardiac vascularized composite allotransplant (VCA). Semi-supervised hierarchical clustering group related...
Abstract SARS-CoV-2 pandemic, the fourth pandemic of decade, has underscored gaps in global preparedness and need for generalizable tests to avert overwhelming healthcare systems worldwide, irrespective a virus. We integrated 4,780 blood transcriptome profiles from patients infected with one 16 viruses across 34 independent cohorts 18 countries, 71 scRNA-seq 264,224 immune cells three cohorts. found myeloid cell-dominated conserved host response associated severity. It showed increased...
Abstract Background Determining the severity of COVID-19 remains an unmet medical need. Our objective was to develop a blood-based host-gene-expression classifier for viral infections and validate it in independent data, including COVID-19. Methods We developed validated multiple infection settings used training data (N=705) from 21 retrospective transcriptomic clinical studies influenza other illnesses looking at preselected panel host immune response messenger RNAs. Results selected 6 RNAs...
Abstract COVID-19 is a pandemic that shares certain clinical characteristics with other acute viral infections. Here, we studied the whole-blood transcriptomic host response to SARS-CoV-2 and compared it infections understand similarities differences in response. Using RNAseq profiled peripheral blood from 24 healthy controls 62 prospectively enrolled patients community-acquired lower respiratory tract infection by SARS-Cov-2 within first hours of hospital admission. We also compiled curated...
Viral pandemics and epidemics pose a significant global threat. While macaque models of viral disease are routinely used, it remains unclear how conserved antiviral responses between macaques humans. Therefore, we conducted cross-species analysis transcriptomic data from over 6,088 blood samples humans infected with one 31 viruses. Our findings demonstrate that irrespective primate or species, there consistent across infection phase (acute, chronic, latent) genome type (DNA RNA viruses)....
Abstract SARS-CoV-2 pandemic, the fourth pandemic of decade, has underscored gaps in global preparedness and need for generalizable tests to avert overwhelming healthcare systems worldwide, irrespective a virus. We integrated 4,780 blood transcriptome profiles from patients infected with one 16 viruses across 34 independent cohorts 18 countries, 71 scRNA-seq 264,224 immune cells three cohorts. found myeloid cell-dominated conserved host response associated severity. It showed increased...
ABSTRACT Viral pandemics and epidemics pose a significant global threat, with emerging re-emerging viruses responsible for four in the 21st century alone. While macaques have been utilized as model understanding viral disease controlled setting, it remains unclear how conserved antiviral responses to diverse are between humans. To address this critical knowledge gap, we conducted comprehensive cross-species analysis of transcriptomic data from over 6000 blood samples humans infected one 31...
Abstract Background COVID-19 is a pandemic caused by the SARS-CoV-2 virus that shares and differs in clinical characteristics of known viral infections. Methods We obtained RNAseq profiles 62 prospectively enrolled patients 24 healthy controls (HC). collected 23 independent studies profiling 1,855 blood samples from covering six viruses (influenza, RSV, HRV, Ebola, Dengue SARS-CoV-1). studied host whole-blood transcriptomic responses compared to non-COVID-19 infections understand...