A5081536768- Extracellular vesicles in disease
- MicroRNA in disease regulation
- DNA Repair Mechanisms
- Viral Infections and Vectors
- Nanoplatforms for cancer theranostics
- Cancer-related molecular mechanisms research
- Mosquito-borne diseases and control
- interferon and immune responses
- Phagocytosis and Immune Regulation
- Carcinogens and Genotoxicity Assessment
- Health and Lifestyle Studies
- Cell Adhesion Molecules Research
- Galectins and Cancer Biology
- Endoplasmic Reticulum Stress and Disease
- Lymphoma Diagnosis and Treatment
- Linguistics and Education Research
- Ferroptosis and cancer prognosis
- Cancer-related Molecular Pathways
- Health, Nursing, Elderly Care
- Psychology and Mental Health
- BRCA gene mutations in cancer
- Trypanosoma species research and implications
- Skin Protection and Aging
- Cancer therapeutics and mechanisms
- Bacterial Genetics and Biotechnology
Champalimaud Foundation
2018-2025
Universidade de São Paulo
1996-2014
Universidade Federal de São Paulo
2007-2011
Extracellular Vesicles (EVs), membrane vesicles released by all cells, are emerging mediators of cell-cell communication. By carrying biomolecules from tissues to biofluids, EVs have attracted attention as non-invasive sources clinical biomarkers in liquid biopsies. EVs-based biopsies usually require isolation before content analysis, which frequently increases sample volume requirements. We here present a Flow Cytometry (FC) strategy that does not or concentration prior staining. doing so,...
Cerebral malaria (CM) is a life-threatening form of Plasmodium falciparum infection caused by brain inflammation. Brain endothelium dysfunction hallmark CM pathology, which also associated with the activation type I interferon (IFN) inflammatory pathway. The molecular triggers and sensors eliciting IFN cellular responses during remain largely unknown. We herein identified stimulator response cGAMP interactor 1 (STING1) as key innate immune sensor that induces Ifnβ1 transcription in mice...
A redução do tempo de sono tem se consolidado como uma condição amplamente comum na sociedade moderna. Estudos recentes têm destacado associações epidemiológicas relevantes entre a alteração nos padrões regulares e o desenvolvimento da obesidade. Ambas as condições possuem um impacto crescente saúde mundial, contribuindo também para aumento risco doenças crônicas. Desse modo, má qualidade ou duração reduzida estão associados alterações metabólicas hormonais que relacionam direta...
Introduction Ferroptosis is a promising new target for ovarian cancer (OVCA) treatment. However, some OVCA cell types resist the induction of ferroptosis by limiting intracellular accumulation labile iron pool (LIP). Methods HEY, COV318 and PEO4 were treated with erastin assessed viability using PI flow cytometry assays. Erastin-affected metabolism was analysed FerroOrange assay, Western Blot (WB) analysis ferritin heavy chain (FtH), transferrin receptor (CD71), ferroportin (FPN)....
ABSTRACT Translational control mediated by phosphorylation of the alpha subunit eukaryotic initiation factor 2 (eIF2α) is central to stress-induced programs gene expression. Trypanosomatids, important human pathogens, display differentiation processes elicited contact with distinct physiological milieu found in their insect vectors and mammalian hosts, likely representing stress situations. Trypanosoma brucei , agent African trypanosomiasis, encodes three potential eIF2α kinases (TbeIF2K1...
Doxorubicin (DOX) is an important tumor chemotherapeutic agent, acting mainly by genotoxic action. This work focus on cell processes that help survival, after DOX-induced DNA damage. In fact, cells deficient for XPA or polymerase eta (pol eta, XPV) proteins (involved in distinct repair pathways) are highly DOX-sensitive. Moreover, LY294002, inhibitor of PIKK kinases, showed a synergistic killing effect these proteins, with strong induction G2/M cycle arrest. Taken together, results indicate...
Yeast Yih1 protein and its mammalian ortholog IMPACT, abundant in neurons, are inhibitors of Gcn2, a kinase involved amino acid homeostasis, stress response, memory formation. Like Yih1/IMPACT harbors an N-terminal RWD domain that mediates binding to the Gcn2 activator Gcn1. competes with for Gcn1 binding, thus inhibiting Gcn2. also binds G-actin. Here, we show Yih1-actin interaction is independent Yih1-Gcn1 does not require actin. The (residues 1–132) was sufficient inhibition but actin...
The protective effect of sunscreens has been extensively evaluated in vivo as a measure erythema induced human skin and is expressed Sun Protection Factor (SPF). In vitro alternatives that use cells might overcome the limitations testing on beings. Here proposed broad accurate approach for evaluating efficacy commercial even under environmental conditions. This Cell dosimeter allowed determination DNA (DNA-SPF), using specific repair enzymes antibodies, Lethal Damage (LD-SPF), by measuring...
Acellular bronchoalveolar lavage (BAL) proteomics can partially separate lung cancer from non-lung patients based on principal component analysis and multivariate analysis. Furthermore, the variance in data sets is correlated mainly with status and, to a lesser extent, smoking gender. Despite these advances BAL small large extracellular vehicles (EVs) proteomes reveal aberrant protein expression paracrine signaling mechanisms initiation progression. We consequently present case-control study...
Diffuse large B cell lymphoma (DLBCL) is an aggressive characterized by a heterogeneous behavior and in need of more accurate biological characterization monitoring prognostic tools. Extracellular vesicles are secreted all types currently established to some extent as representatives the origin. The present study evaluated diagnostic potential plasma extracellular (EVs) proteome DLBCL using state-of-the-art mass spectrometry. EV strongly affected status, with multiple proteins uniquely...
Pancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting matrix remodeling this organ and marked hepatic accumulation of CD11b + bone marrow (BM) cells, which support PC liver metastasis. here show PC-EVs also bind BM cells induce expansion cell population. Transcriptomic characterization these shows upregulate IgG IgA genes, have...
(1) Background: Extracellular vesicles (EVs) have emerged as crucial players in the communication between cells both physiological and pathological scenarios. The functions of EVs are strongly determined by their molecular content, which includes all bioactive molecules, such proteins, lipids, RNA, and, more recently described, double-stranded DNA. It has been shown that oncological settings DNA associated with (EV-DNA) is representative genome parental it reflects mutational status tumor,...
(1) Background: Pancreatic ductal adenocarcinoma (PDAC) is expected to be the second-leading cause of cancer deaths by 2030. Imaging techniques are standard for monitoring therapy response in PDAC, but these have considerable limits, including delayed disease progression detection and difficulty distinguishing benign from malignant lesions. Extracellular vesicle (EV) liquid biopsy an emerging diagnosis modality. Nonetheless, majority research EV-based relies on point analyses EVs at...
Abstract Extracellular Vesicles (EVs), membrane vesicles released by all cells, are emerging mediators of cell-cell communication. By carrying biomolecules from tissues to biofluids, EVs have attracted attention as non-invasive sources clinical biomarkers in liquid biopsies. Although frequently employed for content characterization EVs, the study bulk preparations lacks information on sub-populations and intrinsic heterogeneity vesicles. Importantly, these strategies also difficult small...
Abstract Cerebral malaria (CM) is a life-threatening form of Plasmodium falciparum infection caused by brain inflammation. Brain endothelium dysfunction hallmark CM pathology, which also associated with the activation type I interferon (IFN) inflammatory pathway. The molecular triggers and sensors eliciting IFN cellular responses during remain largely unknown. We herein identified stimulator response cGAMP interactor 1 (STING1) as key innate immune sensor that induces Ifnβ1 transcription in...
This article evaluates an alternative strategy of group intervention using the technique complementing sentences to a students at University just before their examination. It aimed offering nursing undergraduate from College Nursing Ribeirão Preto-University São Paulo, space show feelings related this peculiar situation. The authors searched catch students' emotional conditions regarding expectations as well identify possible aspects that can be handled looking suggesting alternatives...