A5054177918- Insect and Pesticide Research
- Parasitic Infections and Diagnostics
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Entomopathogenic Microorganisms in Pest Control
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Natural Products and Biosynthesis
- Insect-Plant Interactions and Control
- Insect Utilization and Effects
- Microplastics and Plastic Pollution
- Plant Virus Research Studies
- Mycobacterium research and diagnosis
- Advanced Polymer Synthesis and Characterization
- Insect symbiosis and bacterial influences
- Nanoparticles: synthesis and applications
- Polymer composites and self-healing
- Insect and Arachnid Ecology and Behavior
- Photopolymerization techniques and applications
- Silkworms and Sericulture Research
- Silk-based biomaterials and applications
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Molecular Biology Techniques and Applications
- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
Southwest University
2012-2024
Sichuan University of Science and Engineering
2024
State Key Laboratory of Silkworm Genomic Biology
2021-2022
Jiangsu University of Science and Technology
2019-2021
Sericultural Research Institute
2019
Chinese Academy of Agricultural Sciences
2019
The wblA gene, encoding a homologue of the WhiB family protein, was identified in sequenced genome daptomycin producer Streptomyces roseosporus. To explore function wblA, we construct disruption strains, complemented strains and overexpression to test whether it can affect production secondary metabolites morphogenesis.We constructed by homologous recombination S. mutant could block aerial mycelium sporulation enhance 51%. In contrast, resulted significantly decreased yield daptomycin....
Daptomycin, one of the A21978C factors produced by Streptomyces roseosporus, is an acidic cyclic lipopeptide antibiotic with potent activity against a variety Gram-positive pathogens. To increase titre this extensively used and clinically important antibiotic, we applied reported-guided rpsL mutation selection system to generate strains producing high levels A21978C.In reporter design, dptE was chosen as overexpressing target, neo-encoding neomycin phosphotransferase reporter. Using...
A thorough understanding of fungal pathogen adaptations is essential for treating infections. Recent studies have suggested that the role small RNAs expressed in microsporidia genomes are important elucidating mechanisms
Storage proteins are haemolymph-specific in insects, mainly synthesized the fat body, released into haemolymph, and then selectively reabsorbed by body before pupation. These storage play an important role insect metamorphosis egg development. Some of these responsive to pathogen infection can even suppress multiplication. However, mechanisms physiological, biochemical immune-responsive functions remain unclear. In this study, expression patterns Bombyx mori protein 1 (BmSP1) during larval...
Microsporidia are a group of intracellular parasitic eukaryotes, serious pathogens that cause widespread infection in humans, vertebrates, and invertebrates. Because microsporidia have thick spore wall structure, the vitro transformation, cell culture, genetic operation technology far behind other parasites.In this study, according to an analysis life-cycle microsporidia, Nosema bombycis, different electro-transformation conditions, transduction efficiency introducing foreign genes into N....
As microRNAs (miRNAs) are important expression regulators of coding RNA, it is to characterize their role in the interaction between hosts and pathogens. To obtain a comprehensive understanding miRNA alternation Bombyx mori (B. mori) infected with Nosema bombycis (N. bombycis), RNA sequencing stem-loop qPCR were conducted screen identify significantly differentially expressed miRNAs (DEmiRNAs). A total 17 such identified response N. infection, among which miR6498-5p efficiently inhibited...
ABSTRACT Microsporidia are unfriendly microorganisms, and their infections cause considerable damage to economically or environmentally important insects like silkworms honeybees. Thus, the identification of measures improve host resistance microsporidia is critically needed. Here, an overexpressed miR-6498-5p transgenic silkworm line was constructed. Importantly, survival rates median lethal doses were clearly higher after infection with Nosema bombycis . H&E staining RT-qPCR analyses...
Bombyx mori nucleopolyhedrovirus (BmNPV) causes major economic losses in sericulture. A number of agents have been employed to treat viral diseases. Silver nanoparticles (AgNPs) wide applications biomedical fields due their unique properties. The anti-BmNPV effect AgNPs has evaluated, however, there are insufficient studies concerning its toxicity other organisms and the environment. We chemically synthesized biocompatible BSA-AgNPs with a diameter range 2-4 nm characterized physical towards...