- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Ruminant Nutrition and Digestive Physiology
- CRISPR and Genetic Engineering
- Effects of Environmental Stressors on Livestock
- Ovarian function and disorders
- Plant and soil sciences
- Pluripotent Stem Cells Research
- Agricultural and Food Production Studies
- Rabbits: Nutrition, Reproduction, Health
- Agriculture and Rural Development Research
- Reproductive System and Pregnancy
- Sustainable Agricultural Systems Analysis
- Agroforestry and silvopastoral systems
- Cystic Fibrosis Research Advances
- Bacterial Infections and Vaccines
- Male Reproductive Health Studies
- Congenital Anomalies and Fetal Surgery
- Transgenic Plants and Applications
- Animal Nutrition and Physiology
- Agricultural Systems and Practices
- Freezing and Crystallization Processes
Consejo Nacional de Investigaciones Científicas y Técnicas
2020-2025
National Agricultural Technology Institute
2008-2025
Bariloche Atomic Centre
2005-2019
National University of Río Negro
2010-2018
Instituto Superior de Teologia Aplicada
2014
Instituto Nacional de Tecnologia
2010
Possible effects of repeated hormonal treatments and laparoscopic ovum pick-up (LOPU) on the efficiency oocyte recovery rate quality were determined in sheep goats. In six adult Merino five Criolla goats, ovarian status was synchronized by a prostaglandin F(2 alpha) analogue insertion an intravaginal sponge 48 h later. Follicle development stimulated single dose FSH (60 mg NIH-FSH-P1) plus equine chorionic gonadotrophin (eCG; 300 UI). The first FSH/eCG doses administered after insertion,...
Transgenesis is an essential tool in many biotechnological applications. Intracytoplasmic sperm injection (ICSI)-mediated gene transfer a powerful technique to obtain transgenic pups; however, most domestic animal embryos do not develop properly after ICSI. An additional step the protocol, namely assistance by haploid chemical activation, permits use of ICSI-mediated generate preimplantation wide range species, including ovine, porcine, feline, equine and bovine. In present study,...
Transgenesis constitutes an important tool for pharmacological protein production and livestock improvement. We evaluated the potential of laparoscopic insemination (LI), in vitro fertilization (IVF) intracytoplasmic sperm injection (ICSI) to produce egfp-expressing ovine embryos, using spermatozoa previously exposed pCX-EGFP plasmid two different sperm/DNA incubation treatments: "Long Incubation" (2 h at 17 C) "Short (5 min 5 C). For LI, Merino sheep were superovulated inseminated with...
The aim was to evaluate pregnancy success after transfer of embryos vitrified in micropipette tips Merino sheep under extensive conditions. A second objective the influence embryo stage such rate. One hundred and twenty-seven were rewarmed transferred into recipient ewes. On rewarming, placed three-step cryoprotectant dilutions. Finally, prior females, maintained Basic Medium for 5 min at 25ºC re-evaluated by morphological criteria; all degenerated eliminated. Recipient ewes (n = 150)...
Leucocytes from 26 patients with cystic fibrosis (CF) and 18 healthy controls were investigated by migration inhibition induced a variety of antigens. In CF cell-mediated immunity was found to human lung pancreatic tissue extracts as well Aspergillus fumigatus, Pseudomonas aeruginosa, food antigens but not brain, heart, or kidney. Those the severest form disease had greatest impairment immunity, this could be reversed steroid treatment. Cell-mediated cytotoxicity may also concerned in...
Transgenic domestic animals represent an alternative to bioreactors for large-scale production of biopharmaceuticals and could also provide more accurate biomedical models than rodents. However, their generation remains inefficient. Recently, DNA transposons allowed improved transgenesis efficiencies in mice pigs. In this work, Tn5 Sleeping Beauty (SB) transposon systems were evaluated by simple cytoplasmic injection livestock zygotes. the case Tn5, transposome complex nucleic acid protein...
espanolSe evaluo la prenez resultante de inseminacion artificial sistematica cervical (IASC) con semen ovino refrigerado a 5°C durante 12 o 24 h y dosis 150 300 millones espermatozoides. Doscientas ovejas adultas Merino se dividieron al azar en grupos 40 animales, segun arreglo factorial los tratamientos (2x2) mas un grupo control. En estacion reproductiva, estros fueron sincronizados mediante 14 dias esponjas intravaginales 60 mg acetato medroxiprogesterona 200 UI eCG retirar las esponjas....
Se evaluó la preñez resultante de inseminación artificial sistemática cervical (IASC) con semen ovino refrigerado a 5°C durante 12 o 24 h y dosis 150 300 millones espermatozoides. Doscientas ovejas adultas Merino se dividieron al azar en grupos 40 animales, según arreglo factorial los tratamientos (2x2) más un grupo control. En estación reproductiva, estros fueron sincronizados mediante 14 días esponjas intravaginales 60 mg acetato medroxiprogesterona 200 UI eCG retirar las esponjas. A...
The reproductive activity of small ruminants is influenced by genetic and environmental factors that determine different levels seasonality. Due to the large population goats identity Criollo in province Neuquen, Argentina Patagonia, present study was undertaken characterize seasonal variations male activity. During a period one year, ten adult males were evaluated on their parameters. They kept under extensive management conditions natural photoperiod (41° S). Body weight (PV), testicular...
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Resumen es: El conocimiento sobre el inicio de la pubertad y maduracion sexual en una raza o biotipo animal domestico constituye herramienta ne- cesaria pa...