A5088974575- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Mass Spectrometry Techniques and Applications
- Cellular transport and secretion
- Neuroscience and Neuropharmacology Research
- Molecular Communication and Nanonetworks
- Nanopore and Nanochannel Transport Studies
- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- Anesthesia and Neurotoxicity Research
- Enzyme Structure and Function
- Advanced Materials Characterization Techniques
- Catalytic Processes in Materials Science
- Metallurgical Processes and Thermodynamics
- Light effects on plants
- Chemical Synthesis and Characterization
- Analytical Chemistry and Chromatography
- Nicotinic Acetylcholine Receptors Study
- Carbon Nanotubes in Composites
- thermodynamics and calorimetric analyses
- Neural dynamics and brain function
- Radiation Detection and Scintillator Technologies
- Polymer Nanocomposite Synthesis and Irradiation
- Polymer Nanocomposites and Properties
- Diamond and Carbon-based Materials Research
Uppsala University
2024-2025
Forschungszentrum Jülich
2020-2022
European Synchrotron Radiation Facility
2018-2022
National Institute for Research and Development of Isotopic and Molecular Technologies
2018
Moscow Institute of Physics and Technology
2018
Joint Institute for Nuclear Research
2017-2018
Lomonosov Moscow State University
2011-2017
Abstract The light-driven sodium-pumping rhodopsin KR2 from Krokinobacter eikastus is the only non-proton cation active transporter with demonstrated potential for optogenetics. However, existing structural data on correspond exclusively to its ground state, and show no sodium inside protein, which hampers understanding of mechanism. Here we present crystal structure O-intermediate physiologically relevant pentameric form at resolution 2.1 Å, revealing a ion near retinal Schiff base,...
Phytoplankton is the base of marine food chain as well oxygen and carbon cycles thus plays a global role in climate ecology. Nucleocytoplasmic Large DNA Viruses that infect phytoplankton organisms regulate dynamics encompass genes rhodopsins two distinct families. Here, we present functional structural characterization proteins viral rhodopsin group 1, OLPVR1 VirChR1. Functional analysis VirChR1 shows it highly selective, Na+/K+-conducting channel and, contrast to known cation...
Abstract Bin/Amphiphysin/Rvs167 (BAR) domain containing proteins are peripheral membrane that regulate intracellular curvature. BAR protein endophilin B1 plays a key role in multiple cellular processes critical for oncogenesis, including autophagy and apoptosis. Amphipathic regions drive association tubulation through scaffolding. Our understanding of exactly how like promote highly diverse remodeling events the cell is severely limited due to lack high-resolution structural information....
.The work is a review of neutronographic investigations supramolecular structures on upgraded small-angle spectrometer YuMO. Here, key parameters spectrometers are considered. It shown that two-detector system the basis YuMO upgrade. allows to widen dynamic q-range twice. In result, available widened and data collection rate doubled. The detailed description given.The short experimental researches made in polymers field, biology, material science physical chemistry given. current also have...
Using a multidetector system on the YuMO spectrometer allows shortening time of measurements. The quantitative comparison measurement using one and two-detector mode is done. range for experiments was from several minutes up to 12 hours. It shown that shortens more than twice measurement. While making structural investigation advanced software treat data at qualitatively new level. An example illustrating features channels choice shown. results this paper could be used when planning...
Two-component systems (TCS) are widespread signaling present in all domains of life. TCS typically consist a signal receptor/transducer and response regulator. The receptors (histidine kinases, chemoreceptors photoreceptors) often embedded the membrane have similar modular structure. Chemoreceptors were shown to function highly ordered arrays, with trimers dimers being smallest functional unit. However, much less is known about photoreceptors. Here, we use small-angle scattering (SAS) show...
Membrane proteins (MPs) play vital roles in the function of cells and are also major drug targets. Structural information on is for understanding their mechanism critical development drugs. However, obtaining high-resolution structures membrane proteins, particular, under native conditions still a great challenge. In such cases, low-resolution methods small-angle X-ray neutron scattering (SAXS SANS) might provide valuable structural information. some cases (SAS) provides ambiguous ab initio...
Abstract In this work we examine how small hydrophobic molecules such as inert gases interact with membrane proteins (MPs) at a molecular level. High pressure atmospheres of argon and krypton were used to produce noble gas derivatives crystals three well studied MPs (two different proton pumps sodium light-driven ion pump). The structures obtained using X-ray crystallography showed that the vast majority binding sites located on outer surface – usually accommodating chains annular lipids...
Structural properties of HDPE+ZrO2 polymer nanocomposites thin films 80-100μm thicknesses were investigated using SANS, XRD, Laser Raman and FTIR spectroscopy. The mass fraction the filler was 1, 3, 10, 20%. Results XRD analysis showed that ZrO2 powder crystallized both in monoclinic cubic phase under normal conditions. percentages found to be 99.8% 0.2%, respectively. It nanoparticles did not affect main crystal chemical structure HDPE, but degree crystallinity decreases with increasing...
Abstract Phytoplankton is the base of marine food chain, oxygen, carbon cycle playing a global role in climate and ecology. Nucleocytoplasmic Large DNA Viruses regulating dynamics phytoplankton comprise genes rhodopsins two distinct families. We present function-structure characterization homologous proteins representatives family 1 viral rhodopsins, OLPVR1 VirChR1. VirChR1 highly selective, Ca 2+ -dependent, Na + /K - conducting channel and, contrast to known cation channelrhodopsins...
The light-driven sodium pump KR2, found in 2013 the marine bacteria Krokinobacter eikastus, serves as a model protein for studies of sodium-pumping microbial rhodopsins (NaRs). KR2 possesses unique NDQ (N112, D116, and Q123) set amino acid residues functionally relevant positions, named motif. N112 was shown to determine Na+/H+ selectivity pumping efficiency protein. Thus, N112A mutation converts into an outward proton pump. However, no structural data on functional conversions pumps are...
Abstract Bin/Amphiphysin/Rvs (BAR) domain containing proteins are cytosolic, peripheral membrane that regulate the curvature of membranes in eukaryotic cells. BAR protein endophilin B1 plays a key role multiple cellular processes critical for oncogenesis, including autophagy and apoptosis. Amphipathic regions drive association tubulation through scaffolding. Our understanding exactly how like promote highly diverse intracellular remodeling events cell is severely limited due to lack...
<title>Abstract</title> Bin/Amphiphysin/Rvs (BAR) domain containing proteins are cytosolic, peripheral membrane that regulate the curvature of membranes in eukaryotic cells. BAR protein endophilin B1 plays a key role multiple cellular processes critical for oncogenesis, including autophagy and apoptosis. Amphipathic regions drive association tubulation through scaffolding. Our understanding exactly how like promote highly diverse intracellular remodeling events cell is severely limited due...
ABSTRACT Microbial rhodopsins appeared to be the most abundant light-harvesting proteins on Earth and are major contributes solar energy captured in sea. They possess highly diverse biological functions. Explosion of research microbial led breakthroughs their applications, particular, neuroscience. An unexpected new discovery was a Na + -pumping KR2 rhodopsin from Krokinobacter eikastus , first light-driven non-proton cation pump. A fundamental difference between proton other pumps is that...