A5042744064- Metalloenzymes and iron-sulfur proteins
- Physics of Superconductivity and Magnetism
- Enzyme Structure and Function
- Hydrogen Storage and Materials
- Magnetic properties of thin films
- Electrocatalysts for Energy Conversion
- Advanced Condensed Matter Physics
- Theoretical and Computational Physics
- Protein Structure and Dynamics
- Magnetic and transport properties of perovskites and related materials
- X-ray Spectroscopy and Fluorescence Analysis
- Ammonia Synthesis and Nitrogen Reduction
- Catalysis for Biomass Conversion
- Magnetic Properties and Applications
- Superconducting Materials and Applications
- Advanced X-ray Imaging Techniques
- Hemoglobin structure and function
- Rare-earth and actinide compounds
- Nuclear Physics and Applications
- Mass Spectrometry Techniques and Applications
- Distributed and Parallel Computing Systems
- Magnetic Properties of Alloys
- Quantum, superfluid, helium dynamics
- Energy, Environment, Agriculture Analysis
- Particle Accelerators and Free-Electron Lasers
European Synchrotron Radiation Facility
2011-2023
Siemens (Germany)
2017
European Science Foundation
2014
Charité - Universitätsmedizin Berlin
2010-2013
Tergooi
2011
Learning Multi Systems (United States)
2011
Itasca Consultants (Germany)
2010
Laboratoire National des Champs Magnétiques Intenses
1997-2004
Centre National de la Recherche Scientifique
1997-2004
Oracle (United States)
1989-2002
The use of microfluidics on synchrotron X-ray beamlines represents an advanced sample preparation and delivery platform for state-of-the-art characterization micro-samples. recent developments 3D printing technologies have opened possibilities rapid fabrication complex microfluidic devices. One the major challenges in devices using a digital light processing (DLP) desktop printer is that static liquid resin trapped channels, once "ceiling" printed, still receives small doses through...
Abstract Wood and other cellulosic materials are highly sensitive to changes in moisture content, which affects their use most applications. We investigated the effects of on nanoscale structure wood using X-ray neutron scattering, complemented by dynamic vapor sorption. The studied set samples included tension normal hardwood as well representatives two softwood species. Their nanostructure was characterized wet state before after first drying at relative humidities between 15 90%....
Cytomegalovirus disease is an important cause of morbidity following liver transplantation. To date there has not been effective prophylaxis for CMV after One hundred forty-three patients were randomized to receive either high dose oral acyclovir (800 mg 4 times a day) alone 3 months transplantation (acyclovir group) or intravenous ganciclovir (5 mg/kg twice 14 days followed by complete 3-month regimen (ganciclovir group). Of 139 available evaluation, 43 71 (61%) from the group developed...
The phospholipid topology of the outer membrane intact rat liver mitochondria and derived vesicles was investigated by determining accessible pool various classes towards phospholipase A 2 , a phosphatidylcholine‐specific transfer protein chemical labeling using trinitrobenzene sulfonic acid. are sealed have right‐side‐out with proposed localization 55%, 77%, 100%, at least 30% phosphatidylcholine, phosphatidylethanolamine, cardiolipin, phosphatidylinositol plus phosphatidylserine in...
Serial crystallography has enabled the study of complex biological questions through determination biomolecular structures at room temperature using low X-ray doses. Furthermore, it protein dynamics by capture atomically resolved and time-resolved molecular movies. However, many biologically relevant targets is still severely hindered high sample consumption lengthy data-collection times. By combining serial synchrotron (SSX) with 3D printing, a new experimental platform been created that...
Abstract [NiFe] hydrogenases are metalloenzymes catalyzing the reversible heterolytic cleavage of hydrogen into protons and electrons. Gas tunnels make deeply buried active site accessible to substrates inhibitors. Understanding architecture function is pivotal modulating feature O 2 tolerance in a subgroup these hydrogenases, as they interesting for developments renewable energy technologies. Here we describe crystal structure ‐tolerant membrane‐bound hydrogenase Ralstonia eutropha (ReMBH),...
Significance Tracking the route of substrates, intermediates, and inhibitors in proteins is fundamental understanding their specific function. However, following gases like molecular oxygen within enzymes has always been challenging. In protein X-ray crystallography, can be mimicked using krypton or xenon (with a higher electron count); however, these have different physical behavior compared to true substrates/inhibitors. our crystal structure O 2 -tolerant membrane-bound [NiFe] hydrogenase...
A new high-field magnet endstation for X-ray magnetic dichroism experiments has been installed and commissioned at the ESRF soft beamline ID32. The consists of two split-pairs superconducting coils which can generate up to 9 T along beam 4 orthogonal beam. It is connected a cluster ultra-high-vacuum chambers that offer comprehensive set surface preparation characterization techniques. properties have designed provide optimum experimental conditions linear circular in range between 400 1600...
come from teaching and research institutions in France or abroad, public private centers.L'archive ouverte pluridisciplinaire
Molecular oxygen (O 2 ) is a key player in many fundamental biological processes. However, the combination of labile nature and poor affinity O often makes this substrate difficult to introduce into crystals at sufficient concentrations enable protein/O interactions be deciphered detail. To overcome problem, gas pressure cell has been developed specifically for `soak-and-freeze' preparation -dependent molecules. The method uses high molecules or krypton atoms mimics) which, still under...
The specific lattice dynamic properties of antimony in the unfilled $\mathrm{Co}{\mathrm{Sb}}_{3}$ and filled $\mathrm{Eu}{\mathrm{Fe}}_{4}{\mathrm{Sb}}_{12}$ skutterudites have been determined by nuclear inelastic scattering at $^{121}\mathrm{Sb}$ resonance energy $37.1298(2)\phantom{\rule{0.3em}{0ex}}\mathrm{keV}$ with a $4.5\phantom{\rule{0.3em}{0ex}}\mathrm{meV}$ high-resolution backscattering sapphire monochromator. Sb partial vibrational density states (DOS) shows maximum centered 17...
This article describes the High-Pressure Freezing Laboratory for Macromolecular Crystallography (HPMX) at ESRF, and highlights new complementary research opportunities that can be explored using this facility. The laboratory is dedicated to investigating interactions between macromolecules gases in crystallo , finds applications many fields of research, including fundamental biology, biochemistry, environmental medical science. At present, HPMX offers use different high-pressure cells...
The technique of time-resolved macromolecular crystallography (TR-MX) has recently been rejuvenated at synchrotrons, resulting in the design dedicated beamlines. Using pump–probe schemes, this should make mechanistic study photoactive proteins and other suitable systems possible with time resolutions down to microseconds. In order identify relevant delays, spectroscopic experiments directly performed on protein crystals are often desirable. To end, an instrument built ic OS Lab ( crystallo...
The present paper demonstrates the feasibility of X-ray absorption spectroscopy (XAS) and magnetic circular dichroism (XMCD) under high fields up to 26 T low temperatures down 5 K on ID24 energy-dispersive XAS beamline ESRF. pulsed field set-up, entirely developed at ESRF, is described as well synchronization measurement procedure. It allows strengths 30 T. Finally, an example, we report a recent XMCD study Re L2 L3 edges double perovskite Sr2CrReO6.
We have developed a versatile experimental apparatus for synchrotron x-ray measurements in pulsed high magnetic fields. The consists of double cryostat incorporating liquid nitrogen bath to cool the miniature coil and an independent helium flow allowing sample temperatures from 4 up 250K. duty cycle coils can generate 38T. During experiments at 30T repetition rate 6pulses∕min was routinely reached. Using 4kJ power supply, pulse duration between 500μs 1ms. setup used nuclear forward...
A prototype of a high-pressure cooling apparatus dedicated to macromolecular crystallography on synchrotrons is reported. The system allows biological crystals without the addition penetrating or nonpenetrating exogenous cryoprotectant by transforming aqueous solvent into high-density amorphous ice at pressure 200 MPa. samples are directly fished from crystallization trays with cryopins specifically designed for pressurizing device and which compatible robotized sample changers synchrotron...
We present cryo x-ray diffraction microscopy of high-pressure-cryofixed bacteria and report high-convergence imaging with multiple image reconstructions. Hydrated D. radiodurans cells were cryofixed at 200 MPa pressure into $\ensuremath{\sim}10\text{\ensuremath{-}}\ensuremath{\mu}\mathrm{m}$-thick water layers their unstained, hydrated cellular environments imaged by phasing patterns, reaching sub-30-nm resolutions hard x-rays. Comparisons made conventional ambient-pressure-cryofixed...
We have developed a helium gas flow cryostat for use on synchrotron tender to hard X-ray beamlines. Very efficient sample cooling is achieved because the placed directly in removable holder. The compact and easy operate; samples can be changed less than 5 min at any temperature. has temperature range of 2.5–325 K with stability better 0.1 K. very wide optical angle ability operate orientation mean that easily adapted different techniques. It already beamlines European Synchrotron Radiation...
We present a study of heat and charge transport in Bi(2+x)Sr(2-x)CuO(6+delta) focused on the size low-temperature linear term thermal conductivity at optimal-doping level. In superconducting state, magnitude this implies d-wave gap with an amplitude close to what has been reported. normal recovered by application magnetic field, measurement residual resistivity yields Lorenz number L=kappa(N)rho(0)/T=1.3+/-0.2L(0). The departure from value expected Wiedemann-Franz law is thus slightly larger...
Spin and orbital contributions to the magnetic moment of rhenium have been studied across magnetostructural transition in ${\text{Ca}}_{2}{\text{FeReO}}_{6}$ double perovskite employing x-ray circular dichroism (XMCD) at $\text{Re}\text{ }{L}_{2,3}$ edges. Temperature-dependent measurements performed pulsed field varying from 6.8 30 T revealed that two phases this compound are characterized by a different spin-orbit coupling. At $T=10\text{ }\text{K}$, average orbital-to-spin ratio Re...