A5087715176- Enzyme Structure and Function
- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Protein Structure and Dynamics
- RNA Research and Splicing
- Photosynthetic Processes and Mechanisms
- Electrohydrodynamics and Fluid Dynamics
- Algal biology and biofuel production
- Analytical Chemistry and Sensors
- Advanced X-ray Imaging Techniques
- Protein purification and stability
- Plant Molecular Biology Research
- X-ray Spectroscopy and Fluorescence Analysis
- Lipid metabolism and biosynthesis
- Antioxidant Activity and Oxidative Stress
European Synchrotron Radiation Facility
2020-2023
Kurchatov Institute
2015-2019
The use of microfluidics on synchrotron X-ray beamlines represents an advanced sample preparation and delivery platform for state-of-the-art characterization micro-samples. recent developments 3D printing technologies have opened possibilities rapid fabrication complex microfluidic devices. One the major challenges in devices using a digital light processing (DLP) desktop printer is that static liquid resin trapped channels, once "ceiling" printed, still receives small doses through...
As part of its Extremely Brilliant Source (EBS) upgrade project, the ESRF's BM29 BioSAXS beamline was subject to a significant and refurbishment. In addition replacement beamline's original bending magnet source by two-pole wiggler, leading an increase in brilliance factor 60, sample environment almost completely refurbished: vacuum-compatible Pilatus3 X 2M with sensitive area 253.7 mm × 288 frame rates up 250 Hz installed, increasing active available thus q-scaling scattering images taken;...
Liquid-liquid phase separation (LLPS) is an important mechanism enabling the dynamic compartmentalization of macromolecules, including complex polymers such as proteins and nucleic acids, occurs a function physicochemical environment. In model plant,
Re-entrant condensation results in the formation of a condensed protein regime between two critical ion concentrations. The process is driven by neutralization and inversion charge oppositely charged ions. cationic proteins polyvalent anions, pyrophosphate tripolyphosphate, has previously been observed, but not for citrate, which similar size compared to polyphosphates. Therefore, besides electrostatic interactions, other specific interactions polyphosphate ions must contribute. Here, we...
Abstract Liquid-liquid phase separation (LLPS) is an important mechanism enabling the dynamic compartmentalisation of macromolecules, including complex polymers such as proteins and nucleic acids, occurs a function physicochemical environment. In model plant, Arabidopsis thaliana , LLPS by protein EARLY FLOWERING3 (ELF3) in temperature sensitive manner controls thermoresponsive growth. ELF3 contains largely unstructured prion-like domain (PrLD) that acts driver vivo vitro. The PrLD...
Here, we present the design, commissioning, and preliminary results of Thermo-Box, a platform that can maintain precise temperature gradient over time to perform crystallization experiments. Designed be used in conjunction with 3D-printed microfluidic devices, Thermo-Box is laboratory-based unit uses Peltier elements generate large an area accommodate dozen devices. The use devices provides ability test multiple trials facilitates experiments thermally dependent processes without more...