A5038312958- Malaria Research and Control
- Trypanosoma species research and implications
- Cardiomyopathy and Myosin Studies
- Muscle Physiology and Disorders
- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Connective tissue disorders research
- HIV Research and Treatment
- bioluminescence and chemiluminescence research
- Parasitic Infections and Diagnostics
- Cardiovascular Effects of Exercise
- Microbial Community Ecology and Physiology
- X-ray Spectroscopy and Fluorescence Analysis
- Endoplasmic Reticulum Stress and Disease
- Surfactants and Colloidal Systems
- Signaling Pathways in Disease
- Nanoparticle-Based Drug Delivery
- Genetics, Aging, and Longevity in Model Organisms
- Lysosomal Storage Disorders Research
- Ion channel regulation and function
- Enzyme Structure and Function
- Photosynthetic Processes and Mechanisms
- Cancer Research and Treatments
- Electrochemical sensors and biosensors
European Synchrotron Radiation Facility
2022-2024
Institut Curie
2019-2023
Centre National de la Recherche Scientifique
2019-2023
Sorbonne Université
2020-2023
Université Paris Sciences et Lettres
2023
Biologie cellulaire et Cancer
2017
As part of its Extremely Brilliant Source (EBS) upgrade project, the ESRF's BM29 BioSAXS beamline was subject to a significant and refurbishment. In addition replacement beamline's original bending magnet source by two-pole wiggler, leading an increase in brilliance factor 60, sample environment almost completely refurbished: vacuum-compatible Pilatus3 X 2M with sensitive area 253.7 mm × 288 frame rates up 250 Hz installed, increasing active available thus q-scaling scattering images taken;...
Abstract To save energy and precisely regulate cardiac contractility, muscle myosin heads are sequestered in an ‘off’ state that can be converted to ‘on’ when exertion is increased. The equated with a folded-back structure known as the interacting-heads motif (IHM), which regulatory feature of all class-2 non-muscle myosins. We report here human β-cardiac IHM determined by cryo-electron microscopy 3.6 Å resolution, providing details interfaces stabilizing state. shows these hot spots...
Abstract Plasmodium parasites are obligate intracellular protozoa and causative agents of malaria, responsible for half a million deaths each year. The lifecycle progression the parasite is reliant on cell motility, process driven by myosin A, an unconventional single-headed class XIV molecular motor. Here we demonstrate that A from falciparum (PfMyoA) critical red blood invasion. Further, using combination X-ray crystallography, kinetics, in vitro motility assays, elucidate non-canonical...
Abstract Cadherin linkages between adjacent stereocilia and microvilli are essential for mechanotransduction maintaining their organization. They anchored to actin through interaction of cytoplasmic domains with related tripartite complexes consisting a class VII myosin adaptor proteins: Myo7a/SANS/Harmonin in Myo7b/ANKS4B/Harmonin microvilli. Here, we determine high-resolution structures Myo7a Myo7b C-terminal MyTH4-FERM domain (MF2) unveil how they recognize harmonin using novel binding...
Parasites from the genus Plasmodium are causative agents of malaria. The mobility, infectivity, and ultimately pathogenesis falciparum rely on a macromolecular complex, called glideosome. At core glideosome is an essential divergent Myosin A motor (PfMyoA), first order drug target against Here, we present full-length structure PfMyoA in two states its cycle. We report novel interactions that for priming mode recognition light chains (PfELC MTIP) by degenerate IQ motifs. Kinetic motility...
Abstract Malaria results in more than 500,000 deaths per year and the causative Plasmodium parasites continue to develop resistance all known agents, including different antimalarial combinations. The class XIV myosin motor PfMyoA is part of a core macromolecular complex called glideosome, essential for parasite mobility therefore an attractive drug target. Here, we characterize interaction small molecule (KNX-002) with PfMyoA. KNX-002 inhibits ATPase activity vitro blocks asexual blood...
Abstract Structural insights into the photoactivated adenylate cyclases can be used to develop new ways of controlling cellular cyclic adenosine monophosphate (cAMP) levels for optogenetic and other applications. In this work, we use an integrative approach that combines biophysical structural biology methods provide insight on interaction triphosphate (ATP) with dark-adapted state cyclase from cyanobacterium Oscillatoria acuminata (OaPAC). A moderate affinity nucleotide enzyme was...
Lamellarity and shape are important factors in the formation of vesicles determine their role biological systems pharmaceutical applications. Cardiolipin (CL) is a major lipid many membranes exerts great influence on structural organization due to its particular structure physico-chemical properties. Here, we used small-angle X-ray neutron scattering study effects CL with different acyl chain lengths saturations (CL
Summary During normal levels of exertion, many cardiac muscle myosin heads are sequestered in an off-state even during systolic contraction to save energy and for precise regulation. They can be converted on-state when exertion is increased. Hypercontractility caused by hypertrophic cardiomyopathy (HCM) mutations often the result shifting equilibrium toward more on-state. The equated with a folded-back structure known as interacting head motif (IHM), which regulatory feature all myosins...
Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI
Abstract Myosin VI (Myo6) is the only minus-end directed nanomotor on actin, allowing it to uniquely contribute numerous cellular functions. As for other nanomotors, proper functioning of Myo6 relies precise spatiotemporal control motor activity via a poorly defined off-state and interactions with partners. Our structural, functional, studies reveal key features myosin regulation indicate that not all partners can activate Myo6. TOM1 Dab2 cannot bind off-state, while GIPC1 binds Myo6,...
Abstract Malaria is responsible for more than a half million deaths per year. The Plasmodium parasites continue to develop resistance all known agents, despite treatment with different antimalarial combinations. atypical Myosin A motor (PfMyoA) part of core macromolecular complex called the glideosome, essential parasite mobility and therefore an attractive drug target. Here, we characterize interaction small molecule (KNX-002) PfMyoA. KNX-002 inhibits PfMyoA ATPase activity in vitro blocks...
Abstract Myosin VI (Myo6) is the only minus-end directed nanomotor on actin, allowing it to uniquely contribute numerous cellular functions. As for other nanomotors, proper functioning of Myo6 relies precise spatio-temporal control motor activity via a poorly defined off-state and interactions with partners. Our structural, functional, studies reveal key features myosin regulation indicate that not all partners can activate Myo6. TOM1 Dab2 cannot bind while, GIPC1 binds Myo6, releases its...
Abstract Parasites from the genus Plasmodium are causative agents of malaria. The mobility, infectivity and ultimately pathogenesis this parasite relies on a macromolecular complex, called glideosome. At core glideosome is an essential divergent Myosin A motor (PfMyoA), first order drug target against Here we present full-length structure PfMyoA in two states its cycle. We report novel interactions that for priming mode recognition light chains (PfELC MTIP) by degenerate IQ motifs. Kinetic...