A5019285443- Photoreceptor and optogenetics research
- bioluminescence and chemiluminescence research
- Light effects on plants
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Enzyme Structure and Function
- Protein Structure and Dynamics
- X-ray Diffraction in Crystallography
- Molecular Biology Techniques and Applications
- Crystallization and Solubility Studies
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Effects of Radiation Exposure
- Magnetic confinement fusion research
- History of Science and Natural History
- Ion Transport and Channel Regulation
- Dye analysis and toxicity
- Microtubule and mitosis dynamics
- Gold and Silver Nanoparticles Synthesis and Applications
- Genomics and Chromatin Dynamics
- Bone health and osteoporosis research
- Photochromic and Fluorescence Chemistry
- Bone health and treatments
- Bone Metabolism and Diseases
European X-Ray Free-Electron Laser
2022-2024
University Medical Center Hamburg-Eppendorf
2004-2015
Universität Hamburg
2004-2015
Rzeszów University of Technology
2015
Zachodniopomorskie Centrum Onkologii
2004
The understanding of signal transduction mechanisms in photoreceptor proteins is essential for elucidating how living organisms respond to light as environmental stimuli. In this study, we investigated the ATP binding, photoactivation and process photoactivatable adenylate cyclase from Oscillatoria acuminata (OaPAC) upon blue excitation. Structural models with bound active site native OaPAC at cryogenic well room temperature are presented. found one conformation cryogenic- two conformations...
In response to replication stress ATR signaling through CHK1 controls the intra-S checkpoint and is required for maintenance of genomic integrity. Homologous recombination (HR) comprises a series interrelated pathways that function in repair DNA double strand breaks interstrand crosslinks. addition, HR, with its key player RAD51, provides critical support recovery stalled forks during replication. High levels RAD51 are regularly found various cancers, yet little known about effect increased...
Time-resolved crystallography enables the visualization of protein molecular motion during a reaction. Although light is often used to initiate reactions in time-resolved crystallography, only small number proteins can be activated by light. However, many biological triggered interaction between and ligands. The sample delivery method presented here uses mix-and-extrude approach based on 3D-printed microchannels conjunction with micronozzle. diffusive mixing study dynamics samples viscous...
Abstract OaPAC is a recently discovered b lue-light u sing flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses triphosphate and translates light signal into production of cyclic monophosphate. Here, we report crystal structures enzyme in absence its natural substrate determined room temperature serial crystallography data collected at both an X-ray free electron laser synchrotron compare them with cryo macromolecular...
One of the most challenging aspects X-ray research is delivery liquid sample flows into soft beam. Currently, cylindrical microjets are commonly used injection systems for spectroscopy. However, they suffer from several drawbacks, such as complicated geometry due to their curved surface. In this study, we propose a novel 3D-printed nozzle design by introducing microscopic flat sheet jets that provide micrometre-thick sheets with high stability, intending make technology more widely available...
OaPAC is a recently discovered blue-light-using flavin adenosine dinucleotide (BLUF) photoactivated adenylate cyclase from the cyanobacterium Oscillatoria acuminata that uses triphosphate and translates light signal into production of cyclic monophosphate. Here, we report crystal structures enzyme in absence its natural substrate determined room-temperature serial crystallography data collected at both an X-ray free-electron laser synchrotron, compare these with cryo-macromolecular obtained...
<title>Abstract</title> Phytochromes are biliprotein photoreceptors widespread amongst microorganisms and ubiquitous in plants where they control developmental processes as diverse germination, stem elongation floral induction through the photoconversion of inactive Pr to Pfr signalling state. Here we report crystal structures chromophore-binding module soybean phytochrome A, including 2.2Å XFEL well high resolution cryogenic Pr. In structure, chromophore is exposed medium, D-ring remaining...
Abstract The understanding of signal transduction mechanisms in photoreceptor proteins is essential for elucidating how living organisms respond to light as environmental stimuli. In this study, we investigated the ATP binding, photoactivation and process photoactivatable adenylate cyclase from Oscillatoria acuminata (OaPAC) upon blue excitation. Structural models with bound active site native OaPAC at cryogenic well room temperature are presented. found one conformation cryogenic- two...
Abstract Time-resolved crystallography enabled the visualization of protein molecular motion during reaction. While light is commonly used to initiate reactions in time-resolved crystallography, only a small number proteins can fact be activated by light. However, many biological triggered interaction with ligands. The sample delivery method presented here uses mix-and-extrude approach based on 3D printed microchannels conjunction micronozzle study dynamics samples viscous media that...