Abstract This study presents an investigation of the chemical composition Aloe vera leaf tissue with a focus on spatial distribution compounds. The was studied using two mass spectrometry imaging techniques: silver-109 nanoparticles assisted laser desorption/ionization ( 109 AgNPs-LDI-MSI) and ablation-remote atmospheric pressure photoionization/chemical ionization (LARAPPI/CI-MSI) identification aided by ultra-high-performance liquid chromatography ultra-high-resolution (UHPLC-UHRMS)...

Bladder cancer (BC) is one of the most frequently diagnosed types urinary cancer. Despite advances in treatment methods, no specific biomarkers are currently use. Targeted and untargeted profiling metabolites elements human blood serum from 100 BC patients same number normal controls (NCs), with external validation, was attempted using three analytical i.e., nuclear magnetic resonance, gold silver-109 nanoparticle-based laser desorption/ionization mass spectrometry (LDI-MS), inductively...

Renal cell carcinoma (RCC) is the most prevalent and lethal malignancy of kidney. Despite all efforts made, no tissue biomarker currently used in clinical management patients with kidney cancer. A search for possible biomarkers urine clear renal (ccRCC) has been conducted. Non-targeted metabolomic analyses were performed on paired samples surgically removed cancer normal tissue, as well samples. Extracts analyzed by liquid chromatography/high-resolution mass spectrometry (LC-HRMS)....

The laser ablation remote atmospheric pressure photoionization/chemical ionization (LARAPPI/CI) platform coupled to an ultrahigh resolution quadrupole-time-of-flight (QToF) mass spectrometer was developed and employed for the first direct three-dimensional (3D) spectrometry imaging (MSI) of metabolites in human plant tissues. Our solution 3D MSI does not require sample modification or cutting into thin slices. Ablation characteristics optical system based on a diffraction element are studied...

Assessing the spatial distribution of microorganisms’ metabolites in growth medium remains a challenge. Here, we present first use newly developed LARAPPI/CI-MSI 3D (laser ablation remote atmospheric pressure photoionization/chemical ionization mass spectrometry imaging) method for direct three-dimensional (3D) imaging bacterial and fungal solid culture media. Two-dimensional (2D) MSI was also performed, it indicated presence belonging to, including, amino acids their derivatives,...

The aim of this study was to evaluate innovative mass spectrometry imaging (MSI) for determining the metabolic potential selected soil bacteria from genera Bacillus and Priestia in presence phytopathogen Fusarium. This research marks first application direct 3D MSI that visualize interactions between biocontrol agents plant pathogens on agar medium. LARAPPI/CI-3D-MSI (Laser-Assisted Remote Atmospheric Pressure Imaging/Chemical Ionization-3D Mass Spectrometry Imaging) setup provided valuable...

Preparation is described of the surface first monoisotopic cationic 109Ag nanoparticles (AgNPs), covering commercial and modified MALDI targets, along with examples use in MS analyses various low-molecular-weight (LMW) organic compounds, including alkaloids, saccharides, amino acids, nucleosides, nucleic bases, other organics. The new compared to those covered by naturally occurring silver, allow analyte detection higher sensitivity, mass accuracy, resolution. Moreover, triatomic silver...

Renal cell carcinoma (RCC) accounts for several percent of all adult malignant tumor cases and is directly associated with over 120 thousand death worldwide annually. Therefore, there a need cancer biomarker tests methods capable discriminating between normal tissue. It demonstrated that gold nanoparticle enhanced target (AuNPET), nanoparticle-based, surface-assisted laser desorption/ionization (SALDI)-type mass spectrometric method analysis imaging, can differentiate cancerous renal...

Bladder cancer (BC) is a common urological malignancy with high probability of death and recurrence. Cystoscopy used as routine examination for diagnosis following patient monitoring Repeated costly intrusive treatments may discourage patients from having frequent follow-up screenings. Hence, exploring novel non-invasive ways to help identify recurrent and/or primary BC critical. In this work, 200 human urine samples were profiled using ultra-high-performance liquid chromatography...

Although biodeterioration of building materials is a well-recognized problem, unlike heterotrophic organisms the influence aerophytic algae has been so far attributed only to aesthetical and indirect biodeterioration. This article concentrates on determining mixed algal cultures Coenochloris signiensis, Stichococcus bacillaris, Klebsormidium flaccidum Chlorococcum infusionum brick plaster samples, studied in model environment. Inoculated incubated for 28 days samples were examined with...

Infrared (IR) laser ablation-remote-electrospray ionization (LARESI) platform coupled to a tandem mass spectrometer (MS/MS) operated in selected reaction monitoring (SRM) or multiple (MRM) modes was developed and employed for imaging of target metabolites human kidney cancer tissue. SRM MRM were avoid artifacts that are present full scan MS mode. Four tissue samples containing both cancerous noncancerous regions, obtained from three patients with renal cell carcinoma (RCC), imaged. Sixteen...

Despite the awareness that work in sewage treatment plant is associated with biological hazards, they have not been fully recognised so far. The research aims to comprehensively evaluate microbiological and toxicological hazards air settled dust workstations a plant. number of microorganisms was determined using culture method diversity evaluated high-throughput sequencing. Endotoxin concentration assessed GC-MS (gas chromatography-mass spectrometry) while secondary metabolites LC-MS/MS...

Preparation of monoisotopic silver-109 nanoparticles (109AgNPs) by pulsed fiber laser (PFL) ablation synthesis in solution (LASiS) with the use a 2D galvoscanner (2D GS) is described. The procedure covering custom-made stainless-steel MALDI targets containing studied objects via nebulization also presented. Examples application new method (PFL-2D GS LASiS and nebulization) mass spectrometry (MS) analyses MS imaging (MSI) are shown. These include tests nonionic nucleoside saccharide, ionic...

The aim of this case study was the evaluation selected metals’ concentration, potential toxic compound identification, cytotoxicity analysis, estimation airborne dust biodiversity, and number microorganisms in environment (leachate, soil, air) biggest uncontrolled post-industrial landfills Poland. Based on results obtained, preliminary solutions for future management objects that have become an landfill were indicated. In air, PM1 fraction dominated, constituting 78.1–98.2% particulate...

Kidney cancer is one of the most frequently diagnosed and lethal urinary cancer. Despite all efforts made, no serum-specific biomarker currently used in clinical management patients with this tumor. In study, comprehensive high-resolution proton nuclear magnetic resonance spectroscopy (1H NMR) silver-109 nanoparticle-enhanced steel target laser desorption/ionization mass spectrometry (109AgNPET LDI MS) approaches were conducted, conjunction multivariate data analysis, to discriminate global...

Abstract Introduction Kidney cancer is one of the most frequently diagnosed and lethal urinary cancer. Despite advances in treatment, no specific biomarker currently use to guide therapeutic interventions. Objectives Major aim this work was perform metabolomic elemental profiling human kidney normal tissue evaluate biomarkers. Methods Metabolic tumor adjacent from 50 patients with undertaken using three different analytical methods. Results Five potential biomarkers were identified...

This study aimed to assess the physicochemical, microbiological and toxicological hazards at an illegal landfill in central Poland. The research included analysis of airborne dust (laser photometer), number microorganisms air, soil leachate (culture method) microbial diversity environment (high-throughput sequencing on Illumina Miseq); cytotoxicity (PrestoBlue) genotoxicity (alkaline comet assay) were tested. Moreover, UHPLC-Q-ToF-UHRMS (ultra-high-performance liquid...