Assessing the spatial distribution of microorganisms’ metabolites in growth medium remains a challenge. Here, we present first use newly developed LARAPPI/CI-MSI 3D (laser ablation remote atmospheric pressure photoionization/chemical ionization mass spectrometry imaging) method for direct three-dimensional (3D) imaging bacterial and fungal solid culture media. Two-dimensional (2D) MSI was also performed, it indicated presence belonging to, including, amino acids their derivatives,...

The aim of this study was to evaluate innovative mass spectrometry imaging (MSI) for determining the metabolic potential selected soil bacteria from genera Bacillus and Priestia in presence phytopathogen Fusarium. This research marks first application direct 3D MSI that visualize interactions between biocontrol agents plant pathogens on agar medium. LARAPPI/CI-3D-MSI (Laser-Assisted Remote Atmospheric Pressure Imaging/Chemical Ionization-3D Mass Spectrometry Imaging) setup provided valuable...

In this work, beeswax was used for the first time finishing polyester/Cotton/Viscose blend fabric and polyester fabric. The aims of study were: (1) to characterize composition (using Gas Chromatography Mass Spectrometry, GC-MS 109AgNPET laser desorption/ionization mass spectrometry (LDI MS); (2) develop a laboratory method applying beeswax; (3) assess antimicrobial activity fabrics against bacteria fungi (AATCC 100–2004 test); (4) properties textiles modified by beeswax. Beeswax composed...

Despite the awareness that work in sewage treatment plant is associated with biological hazards, they have not been fully recognised so far. The research aims to comprehensively evaluate microbiological and toxicological hazards air settled dust workstations a plant. number of microorganisms was determined using culture method diversity evaluated high-throughput sequencing. Endotoxin concentration assessed GC-MS (gas chromatography-mass spectrometry) while secondary metabolites LC-MS/MS...

The aim of this case study was the evaluation selected metals’ concentration, potential toxic compound identification, cytotoxicity analysis, estimation airborne dust biodiversity, and number microorganisms in environment (leachate, soil, air) biggest uncontrolled post-industrial landfills Poland. Based on results obtained, preliminary solutions for future management objects that have become an landfill were indicated. In air, PM1 fraction dominated, constituting 78.1–98.2% particulate...

Abstract Mudstones and claystones from the southern marginal area of European Upper Triassic, midcontinental Keuper basin (Silesia, Poland) were investigated using XRD, organic inorganic geochemistry, SEM, K-Ar illite-smectite, AFT, stable isotopes O C in carbonates order to unravel consequent phases geological history these rocks, known for abundant fossils land vertebrates, particular evaluate diagenetic overprint on mineral composition. The detected quantified assemblage consists quartz,...

This study aimed to assess the physicochemical, microbiological and toxicological hazards at an illegal landfill in central Poland. The research included analysis of airborne dust (laser photometer), number microorganisms air, soil leachate (culture method) microbial diversity environment (high-throughput sequencing on Illumina Miseq); cytotoxicity (PrestoBlue) genotoxicity (alkaline comet assay) were tested. Moreover, UHPLC-Q-ToF-UHRMS (ultra-high-performance liquid...

Lactic acid bacteria (LAB) are an essential part of the microbiota digestive tract honeybees (Apis mellifera L.). Antagonistic activity 103 LAB strains (isolates from different environments) against 21 honeybee pathogens/opportunistic pathogens (with agar slab method) was screened. The growth Paenibacillus genus inhibited to most extent. highest antagonistic demonstrated by Lacticaseibacillus casei 12AN, while lowest Apilactobacillus kunkeei DSM 12361, a species naturally inhabiting gut....

This study aimed to assess the markers of chemical and microbiological contamination air at sport centers (e.g., fitness center in Poland) including determination particulate matter, CO2, formaldehyde (DustTrak™ DRX Aerosol Monitor; Multi-functional Air Quality Detector), volatile organic compound (VOC) concentration (headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry), number microorganisms (culture methods), microbial biodiversity (high-throughput...

The aim of the present study was to evaluate relation between chemical (analysis elements and pH) microbiological composition (culture metagenomics analysis) dust at various workplaces (cement plant, composting poultry farm, cultivated area) cytotoxicity effect on human adenocarcinoma lung epithelial adherent cell line A-549 (MTT assay test). Analysis Particulate Matter (PM) fraction showed that concentration in areas exceeded OELs. For remaining examined, lower than OELs limits. number...

We analysed how body mass, migration distance, taxonomic family and breeding on small islands vs. continents affect wing length in 72 species of closely related Old World reed bush warblers (Acrocephalidae Locustellidae), based literature data. The we share similar morphology, habitat, food preferences, feeding habits systems, but their migratory behaviour varies from sedentariness to long-distance migration. mean these was strongly correlated with distance (R 2 = 0.824; p 3.2 –26). average...

This study, which was conducted on a farm in central Poland from February till April 2019, aimed to evaluate microbiological threats cattle breeding premises by counting and identifying the microorganisms (via culturing high-throughput sequencing), assessing endotoxin concentrations gas chromatography-mass spectrometry [GC-MS]) analysing secondary metabolites (including mycotoxins) air sedimented dust liquid chromatography-tandem mass [LC-MS/MS]) six barns. In addition, cytotoxicity of human...

The growth of population and increase in diseases that cause an enormous demand for biomedical material consumption is a pointer to the pressing need develop new sustainable biomaterials. Electrospun materials derived from green polymers have gained popularity recent years applications such as tissue engineering, wound dressings, drug delivery. Among various bioengineering used synthesis biodegradable polymer, poly(lactic acid) (PLA) has received most attention researchers. Hypericum...

An increasing trend in protecting plants against phytopathogens is the replacement of chemical pesticides with environmentally acceptable biopreparations. This article focuses on possible use yeast Metschnikowia pulcherrima as a biocontrol agent potato pathogens. The scope included an assessment antimicrobial activity 10 M. isolates phytopathogens: Fusarium oxysporum, sambucinum, Rhizoctonia solani, Alternaria Alternaria, tenuissima, alternata, Colletotrichum coccodes, Phoma exigua,...

The aim of this study was to analyse the quality indoor air in sport facilities one centres Poland with respect microclimatic parameters (temperature, humidity, and flow velocity), particulate matter concentrations (PM10, PM4, PM2.5, PM1), gas (oxygen, ozone, hydrogen sulphide, sulphur dioxide, volatile organic compounds, benzopyrene), microbial contamination (the total number bacteria, specifically staphylococci, including Staphylococcus aureus, haemolytic Enterobacteriaceae, Pseudomonas...

In the last decade, fresh-cut plants have become a more popular flavoring additive in food. It is important to find an effective method for ensuring safety and quality of plant materials used as food additives. Ozonated water being considered by EFSA approval cidal agent protection. The objective this study was evaluate effectiveness ozonated improving microbial parsley leaves, with particular focus on mesophilic psychrotrophic bacteria fungi. yeasts were identified MALDI-TOF MS system....