A5058781354- Magnetic properties of thin films
- Quantum Dots Synthesis And Properties
- Advanced Electron Microscopy Techniques and Applications
- Surface and Thin Film Phenomena
- Complement system in diseases
- Enzyme Structure and Function
- Magnetic Properties and Applications
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Chalcogenide Semiconductor Thin Films
- Calcium Carbonate Crystallization and Inhibition
- Electron and X-Ray Spectroscopy Techniques
- Perovskite Materials and Applications
- nanoparticles nucleation surface interactions
- Advanced biosensing and bioanalysis techniques
- Theoretical and Computational Physics
- Photosynthetic Processes and Mechanisms
- Nanoparticle-Based Drug Delivery
- Nanoparticles: synthesis and applications
- Graphene and Nanomaterials Applications
- Quantum and electron transport phenomena
- Nanocluster Synthesis and Applications
- Blood groups and transfusion
- Advanced Chemical Physics Studies
- Protein Structure and Dynamics
CEA Grenoble
2015-2024
Institut de Biologie Structurale
2015-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2024
Université Grenoble Alpes
2015-2024
Centre National de la Recherche Scientifique
2015-2024
Institut de Recherche Interdisciplinaire de Grenoble
2019
CEA LETI
2009-2012
Institut polytechnique de Grenoble
2009-2012
Université Joseph Fourier
2009-2011
Sandia National Laboratories California
2004-2006
Nonpyrophoric aminophosphines reacted with indium(III) halides in the presence of zinc chloride have emerged as promising phosphorus precursors synthesis colloidal indium phosphide (InP) quantum dots (QDs). Nonetheless, due to required P/In ratio 4:1, it remains challenging prepare large-sized (>5 nm), near-infrared absorbing/emitting InP QDs using this synthetic scheme. Furthermore, addition leads structural disorder and formation shallow trap states inducing spectral broadening. To...
Measles is a highly contagious human disease. We used cryo-electron microscopy and single particle-based helical image analysis to determine the structure of nucleocapsid formed by folded domain measles virus nucleoprotein encapsidating an RNA at resolution 4.3 angstroms. The resulting pseudoatomic model offers important insights into mechanism polymerization nucleocapsids negative-strand viruses, in particular via exchange subdomains nucleoprotein. reveals mode nucleoprotein-RNA interaction...
Particle size distribution and stability are key attributes for the evaluation of safety efficacy profile medical nanoparticles (Med-NPs). Measuring particle average is a challenging task which requires combination orthogonal high-resolution sizing techniques, especially in complex biological media. Unfortunately, despite its limitations, due to accessibility, low cost, easy handling, batch mode dynamic light scattering (DLS) still very often used as only approach measure nanomedicine field....
Liquid-liquid phase separation (LLPS) is an important mechanism enabling the dynamic compartmentalization of macromolecules, including complex polymers such as proteins and nucleic acids, occurs a function physicochemical environment. In model plant,
In the endoplasmic reticulum, calreticulin acts as a chaperone and Ca2+-signalling protein. At cell surface, it mediates numerous important biological effects. The crystal structure of human globular domain was solved at 1.55 Å resolution. Interactions flexible N-terminal extension with edge lectin site are consistently observed, revealing hitherto unidentified peptide-binding site. A molecular zipper, observed in all lattices, could further extend this by creating binding cavity lined...
With the goal to tune emission properties of colloidal InP quantum dots, incorporation Ga was explored. Unexpectedly, depending on nature gallium precursor, photoluminescence shifted either red (gallium oleate) or blue acetylacetonate). In first case, larger-sized InP/GaP core/shell nanocrystals were formed, while in second case formation an InGaP alloy structure enabled range (475 nm) be accessed.
With the goal to improve their photostability, InP-based QDs are passivated with three types of inorganic shells, namely i) a gradient ZnSe(x)S(1-x) shell, ii) an additional ZnS shell on top two different thicknesses (core/shell/shell, CSS), iii) alumina coating ZnS. All systems have photoluminescence quantum yields (PLQY) > 50% and similar PL decay times (64-67 ns). To assess photostability they incorporated into transparent poly(methyl methacrylate) (PMMA) matrix exposed continuous...
Downstream processing (DSP) of large bionanoparticles is still a challenge. The present study aims to systematically compare some the most commonly used DSP strategies for capture and purification enveloped viruses virus-like particles (eVLPs) by using same staring material analytical tools. As model, Human Immunodeficiency Virus-1 (HIV-1) gag VLPs produced in CHO cells were used. Four different tested. An anion-exchange monolith membrane adsorber, direct eVLPs, polymer-grafted resin...
Abstract Only praziquantel is available for treating schistosomiasis, a disease affecting more than 200 million people. Praziquantel-resistant worms have been selected in the lab and low cure rates from mass drug administration programs suggest that resistance evolving field. Thioredoxin glutathione reductase (TGR) essential schistosome survival validated target. TGR inhibitors identified to date are irreversible and/or covalent with unacceptable off-target effects. In this work, we identify...
The classical pathway of complement is an essential component the human innate immune system involved in defense against pathogens as well clearance altered self-components. Activation this triggered by C1, a multimolecular complex comprising recognition protein C1q associated with catalytic subunit C1s-C1r-C1r-C1s. We report here direct observation organized binding C1 components and C1s-C1r-C1r-C1s on carbon nanotubes, ubiquitous nanotechnology research. Electron microscopy imaging showed...
Biocompatibility, biofunctionality, and chemical stability are essential criteria to be fulfilled by quantum dot (QD) emitters for bio-imaging -sensing applications. In addition these criteria, achieving efficient near-infrared (NIR) emission with nontoxic QDs remains very challenging. this perspective, we developed water-soluble NIR-emitting AgInS2/ZnS core/shell (AIS/ZnS) functionalized DNA. The newly established aqueous route relying on a two-step hot-injection synthesis led highly...
Abstract Self-assembly of proteins holds great promise for the bottom-up design and production synthetic biomaterials. In conventional approaches, designer are pre-programmed with specific recognition sites that drive association process towards a desired organized state. Although proven effective, this approach poses restrictions on complexity material properties end-state. An alternative, hierarchical has found wide adoption inorganic systems, relies crystalline nanoparticles become...
Continuous flow synthesis of semiconductor quantum dots (QDs) holds the promise being highly reproducible, scalable, and providing precise control all reaction parameters. Here, we applied this technique to aqueous Ag–In–S (AIS) core AIS/ZnS core/shell QDs optimized several parameters comprising temperature, pressure, time, nature, ratio precursors. Photoluminescence yield (PLQY) values 32%/44% (average/best) for 77%/83% system have been obtained in short times (8–15 min). We demonstrate by...
Multiple-step nucleation pathways have been observed during mineral formation in both inorganic and biomineral systems. These can involve precursor aqueous species, amorphous intermediates, or metastable phases. Despite the widespread occurrence of these processes, elucidating precise steps transformation mechanisms between each step remains a challenging task. Using suite potentiometric, microscopic, spectroscopic tools, we studied pathway SrSO4 as function physico-chemical solution...
Lead halide perovskite (LHP) based colloidal quantum dots (CQDs) have tremendous potential for photocatalysis due to their exceptional optical properties. However, applicability in catalysis is restricted poor chemical stability and low recyclability. We report halide-passivated, monodisperse CsPbBr
Abstract Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis jegathesan , respectively. The naturally crystallize within host; however, crystals too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. was determined de novo using single-wavelength anomalous dispersion method, which turn...
We find that less than 0.01 monolayer of S can enhance surface self-diffusion on Cu(111) by several orders magnitude. The measured dependence two-dimensional island decay rates coverage (theta(S)) is consistent with the proposal Cu3S3 clusters are responsible for enhancement. Unexpectedly, and ripening diffusion limited very low high theta(S) but not intermediate theta(S). To explain this result we propose mass transport in region rate reaction to form terraces.
Poxviridae are viruses with a large linear double-stranded DNA genome coding for up to 250 open reading frames and fully cytoplasmic replication. The is covalently circularized at both ends. Similar structures of linked extremities the found in African swine fever virus (asfarvirus) Phycodnaviridae We studying machinery which replicates this peculiar structure. From our work vaccinia virus, we give first insights into overall structure function essential poxvirus helicase-primase D5 show...
Polymer-grafted chromatography media, especially ion exchangers, are high performance materials for protein purification. However, due to the pore size limitation, conventional beads usually not considered downstream processing of large biomolecules such as virus-like particles (VLPs). Contrariwise, since outer surface provides satisfactory binding capacity VLPs and impurities smaller can bind inside beads, porous should be VLP capture We used HIV-1 gag with a diameter 100–200 nm model...
Many attempts have been made to synthesize cadmium-free quantum dots (QDs), using nontoxic materials, while preserving their unique optical properties. Despite impressive advances, gaps in knowledge of intracellular fate, persistence, and excretion from the targeted cell or organism still exist, precluding clinical applications. In this study, we used a simple model (Hydra vulgaris) presenting tissue grade organization determine biodistribution indium phosphide (InP)-based QDs by X-ray...