A5070293009- Crystallization and Solubility Studies
- Lanthanide and Transition Metal Complexes
- X-ray Diffraction in Crystallography
- Historical Studies and Socio-cultural Analysis
- Medieval and Early Modern Justice
- European Political History Analysis
- Enzyme Structure and Function
- Radiopharmaceutical Chemistry and Applications
- Protein Structure and Dynamics
- Magnetism in coordination complexes
- Luminescence and Fluorescent Materials
- Metal complexes synthesis and properties
- Molecular Sensors and Ion Detection
- Reformation and Early Modern Christianity
- Crystallography and molecular interactions
- Medieval European Literature and History
- Medical Imaging Techniques and Applications
- Parkinson's Disease Mechanisms and Treatments
- Blood properties and coagulation
- Amino Acid Enzymes and Metabolism
- Hemoglobinopathies and Related Disorders
- Historical and Linguistic Studies
- Nanocluster Synthesis and Applications
- Chemical Synthesis and Analysis
- Advanced biosensing and bioanalysis techniques
Université d'Évry Val-d'Essonne
2024
École Normale Supérieure de Lyon
2018-2024
Université Claude Bernard Lyon 1
2014-2024
Laboratoire de Chimie
2001-2024
Centre National de la Recherche Scientifique
2012-2024
Université Paris 1 Panthéon-Sorbonne
2008-2020
Van Andel Institute
2020
Institut Pluridisciplinaire Hubert Curien
2014-2019
Institut de Chimie de Clermont-Ferrand
2019
Université de Strasbourg
2017-2019
In addition to the already described ligand L4a, two pyclen-based lanthanide chelators, L4b and L4c, bearing specific picolinate two-photon antennas (tailor-made for each targeted metal) one acetate arm arranged in a dissymmetrical manner, have been synthesized, form complete family of luminescent bioprobes: [EuL4a], [SmL4a], [YbL4b], [TbL4c], [DyL4c]. Additionally, symmetrically regioisomer L4a′ was also synthesized as well its [EuL4a′] complex highlight astonishing positive impact...
The design, synthesis, and application of a nine-coordinate gadolinium(III)-containing spin label, [Gd.sTPATCN]-SL, for use in nanometer-distance measurement experiments by EPR spectroscopy is presented. label links to cysteines via short thioether tether has narrow central transition indicative small zero-field splitting (ZFS). A protein homodimer, TRIM25cc, was selectively labeled with [Gd.sTPATCN]-SL (70%) nitroxide (30%) under mild conditions measured using the double electron resonance...
Abstract The design of original twisted charge transfer antennae in which a non‐planar geometry is enforced thanks to one or two bulky ortho ‐Me substituents allows us prepare the corresponding ultra‐bright Tb III and Dy bioprobes. brightness derivative compares well with that benchmark Tb‐Lumi4 complex. first bio‐imaging experiments luminescent bioprobe are also reported.
Pyclen-based ligand bearing picolinate ILCT antenna forms highly luminescent one- and two-photon Eu(<sc>iii</sc>) Sm(<sc>iii</sc>) complexes.
Abstract Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis jegathesan , respectively. The naturally crystallize within host; however, crystals too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. was determined de novo using single-wavelength anomalous dispersion method, which turn...
Abstract A prototype luminescent turn‐on probe for Cu + (and Ag ) is described, harnessing a selective binding site (log K ass =9.4 and 7.3 , respectively) based on the coordinating environment of bacterial metallo‐chaperone CusF, integrated with terbium‐ion‐signaling moiety. Cation–π interactions were shown to enhance tryptophan triplet population, which subsequently sensitized, microsecond timescale, long‐lived terbium emission, offering novel approach in bioinspired chemosensor design.
A family of 2,4-pyridyl-disubstituted bispidol derivatives bearing methylene carboxylic acid ethyl esters (L1–L3), acids (L4 and L5), or methylenethiophene (L6) groups were synthesized. In water, all ligands form rigid 1:1 complexes in the presence Zn(II) which bicycle adopts a chair–chair conformation (cis isomer), as observed by 1H NMR and, case ligand L1, an X-ray diffraction crystal structure. Interestingly, addition ions on L1 induces metal-mediated selective hydrolysis esters. This was...
Abstract Responsive luminescent probes emitting in the near‐infrared (NIR) are high demand today for biological applications as they allow easy and unambiguous discrimination of autofluorescence. Due to their luminescence properties, lanthanide ions offer an interesting alternative classical organic fluorescent dyes. This has stimulated development lanthanide‐based responsive probes. Nevertheless, that can operate water with NIR‐emitting scarce. In this communication, zinc fingers shown be...
Here we present the synthesis and characterization of a new bispidine (3,7-diazabicyclo[3.3.1]nonane) ligand with N-methanephosphonate substituents (L2). Its physicochemical properties in water, as well those corresponding Cu(II) Zn(II) complexes, have been evaluated by using UV-visible absorption spectroscopy, potentiometry, 1H 31P NMR, cyclic voltammetry. Radiolabeling experiments 64CuII carried out, showing excellent radiolabeling properties. Quantitative complexation was achieved within...
EuIII , TbIII GdIII and YbIII complexes of the nonadentate bispidine derivative L2 (bispidine=3,7-diazabicyclo[3.3.1]nonane) were successfully synthesized their emission properties studied. The X-ray crystallography reveals full encapsulation by ligand that enforces to all LnIII cations a common highly symmetrical capped square antiprismatic (CSAPR) coordination geometry (pseudo C4v symmetry). well-resolved identical spectra in solid state solution confirm equal structures both media. As...
Cationic lanthanide complexes are generally able to spontaneously internalize into living cells. Following our previous works based on a diMe-cyclen framework, second generation of cationic water-soluble constrained cross-bridged cyclam macrocycle functionalized with donor-π-conjugated picolinate antennas was prepared europium(III) and ytterbium(III). Their spectroscopic properties were thoroughly investigated in various solvents rationalized the help DFT calculations. A significant...
Small Rigid Platforms (SRPs) are sub-5 nanometre gadolinium based nanoparticles that have been developed for multimodal imaging and theranostic applications. They composed of a polysiloxane network surrounded by chelates. A covalent coupling with quinoxaline derivatives has performed. Such proven their affinity melanin frequently expressed in primary melanoma cases. Three different synthesised coupled to the nanoparticles. The grafted then shown vitro surface plasmon resonance on homemade gold chip.
The first radiolabelling studies of a bispidine (3,7-diazabicyclo[3.3.1]nonane) derivative substituted by glycinate pendant arm (L1) with 64Cu are reported. Labelling was fast and easily performed at room temperature in wide range pH values. Under these conditions, radiochemical yields over 90% were achieved within 5 minutes micromolar concentration the ligand. A bifunctional analogue L1 (L2) has been obtained introducing an l-lysine amino acid on skeleton. Ligand L2 demonstrates good...
Three new bispidone derivatives substituted by methylenecarboxylic ethyl ester groups have been synthesized in high yields as potential ligands for 64Cu complexation and PET imaging. Their solution solid-state structures determined 1H NMR spectroscopy X-ray crystallography. These studies reveal a strong rigidity of the bicycle, which adopts either chair–chair or boat–chair conformation depending on substituents N3 N7 positions. A methyl substituent at stabilizes conformation, whereas...
The supramolecular interaction between lanthanide complexes and proteins is at the heart of numerous chemical biological studies. Some these have demonstrated remarkable properties with or peptides in solution crystalline state. Here we used paramagnetism ions to characterize affinity two for ubiquitin. As process dynamic, acquired NMR data only reflect time average different steps. We molecular dynamics (MD) simulations get a deeper insight into detailed scenario microsecond scale. This...
Crystallophores are lanthanide complexes that have demonstrated outstanding induction of crystallization for various proteins. This article explores the effect tailored modifications crystallophore first generation and studies their impact on nucleating properties, protein crystal structures. Through high-throughput experiments dataset analysis, we evaluated effectiveness these variants, in comparison to G1. In particular, V1 variant, featuring a propyl-3-ol pendant arm, ability produce new...
We study the supramolecular interaction between ubiquitin and lanthanide complexes by paramagnetic NMR molecular dynamics. Our observations shed new light on dynamic processes these surface of protein.
Abstract A prototype luminescent turn‐on probe for Cu + (and Ag ) is described, harnessing a selective binding site (log K ass =9.4 and 7.3 , respectively) based on the coordinating environment of bacterial metallo‐chaperone CusF, integrated with terbium‐ion‐signaling moiety. Cation–π interactions were shown to enhance tryptophan triplet population, which subsequently sensitized, microsecond timescale, long‐lived terbium emission, offering novel approach in bioinspired chemosensor design.
Mutations of the gene encoding major component Lewy bodies (LB), α-synuclein (α-syn), cause autosomal dominant forms Parkinson's disease (PD), whereas loss-of-function mutations multifunctional E3 ubiquitin-protein ligase Parkin account for recessive disease. overproduction protects against α-syn-dependent neurodegeneration in various vitro and vivo models, but it remains unclear whether this process is affected by deficiency. We addressed issue, carrying out more detailed analyses...
Background: Synucleinopathy is a group of neurodegenerative disorders characterized by neurodegeneration and accumulation alpha-synuclein (α-syn) aggregates in various brain regions. The detailed mechanism α-syn-caused neurotoxicity remains obscure, which partly due to the lack suitable model that retains vivo three-dimensional cellular network allows convenient dissection neurotoxic pathways. Recent studies revealed pre-formed recombinant α-syn amyloid fibrils (PFFs) induce robust...