A5033027063- Gut microbiota and health
- Tryptophan and brain disorders
- Neuroendocrine regulation and behavior
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Hemoglobin structure and function
- Advanced NMR Techniques and Applications
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Protein Structure and Dynamics
- Microbial Community Ecology and Physiology
- Advanced Electron Microscopy Techniques and Applications
- Marine and coastal ecosystems
- Radioactive element chemistry and processing
- Light effects on plants
- Protist diversity and phylogeny
- Iron oxide chemistry and applications
European Synchrotron Radiation Facility
2022-2024
Universität Hamburg
2022-2024
Université Côte d'Azur
2021
Institut de Pharmacologie Moléculaire et Cellulaire
2020-2021
Autism spectrum disorders (ASD) are associated with dysregulation of the microbiota-gut-brain axis, changes in microbiota composition as well fecal, serum, and urine levels microbial metabolites. Yet a causal relationship between axis ASD remains to be demonstrated. Here, we hypothesized that metabolite p-Cresol, which is more abundant patients compared neurotypical individuals, could induce ASD-like behavior mice.Mice exposed p-Cresol for 4 weeks drinking water presented social deficits,...
Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism photolyase-catalyzed cyclobutane pyrimidine dimer (CPD) lesion using time-resolved serial femtosecond crystallography (TR-SFX). We obtained 18 snapshots that show time-dependent changes in four reaction loci. used these results create movie depicts CPD lesions picosecond-to-nanosecond range, followed by recovery enzymatic moieties involved...
Over the last decade, development of time-resolved serial crystallography (TR-SX) at X-ray free-electron lasers (XFELs) and synchrotrons has allowed researchers to study phenomena occurring in proteins on femtosecond-to-minute timescale, taking advantage many technical methodological breakthroughs. Protein crystals various sizes are presented beam either a static or moving medium. Photoactive were naturally initial systems be studied TR-SX experiments using pump–probe schemes, where pump is...
The marine cyanobacterium Prochlorococcus is a main contributor to global photosynthesis, whilst being limited by iron availability. Cyanobacterial genomes generally encode two different types of FutA iron-binding proteins: periplasmic FutA2 ABC transporter subunits bind Fe(III), while cytosolic FutA1 binds Fe(II). Owing their small size and economized genome ecotypes typically possess single futA gene. How the encoded protein might Fe oxidation states was previously unknown. Here, we use...
The technique of time-resolved macromolecular crystallography (TR-MX) has recently been rejuvenated at synchrotrons, resulting in the design dedicated beamlines. Using pump–probe schemes, this should make mechanistic study photoactive proteins and other suitable systems possible with time resolutions down to microseconds. In order identify relevant delays, spectroscopic experiments directly performed on protein crystals are often desirable. To end, an instrument built ic OS Lab ( crystallo...
The development of serial crystallography over the last decade at XFELs and synchrotrons has produced a renaissance in room-temperature macromolecular (RT-MX), fostered many technical methodological breakthroughs designed to study phenomena occurring proteins on picosecond-to-second timescale. However, there are components protein dynamics that occur much slower regimes, which could readily benefit from state-of-the-art RT-MX. Here, structural relaxation reaction intermediate synchrotron,...
ABSTRACT Microbial rhodopsins are omnipresent on Earth, however the vast majority of them remain uncharacterized. Here we describe a new rhodopsin group from cold-adapted organisms and cold environments, such as glaciers, denoted CryoRhodopsins (CryoRs). Our data suggest that CryoRs have dual functionality switching between inward transmembrane proton translocation photosensory activity, both which can be modulated with UV light. CryoR1 exhibits two subpopulations in ground state, upon light...
ABSTRACT Background Autism Spectrum Disorders (ASD) are associated with dysregulation of the microbiota-gut-brain axis resulting in changes microbiota composition as well fecal, serum and urine levels microbial metabolites. Yet, a causal relationship between ASD remains to be demonstrated. Here, we hypothesized that metabolite p -Cresol, which is more abundant patients compared neurotypical individuals, could induce ASD-like behavior mice. Results Mice exposed -Cresol for 4 weeks drinking...
Abstract The marine cyanobacterium Prochlorococcus is a main contributor to global photosynthesis, whilst being limited by iron availability. Cyanobacterial genomes typically encode two different types of FutA binding proteins: periplasmic FutA2 ABC transporter subunits bind Fe(III), while cytosolic FutA1 binds Fe(II). Owing their small size and economized genome ecotypes possess single futA gene. How the encoded protein might Fe oxidation states was previously unknown. Here we use...