A5091081243- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Biochemical Analysis and Sensing Techniques
- Enzyme Structure and Function
- Ubiquitin and proteasome pathways
- DNA and Nucleic Acid Chemistry
- Light effects on plants
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Chemical Synthesis and Analysis
- Photoreceptor and optogenetics research
- Heat shock proteins research
- Trace Elements in Health
- Lipid Membrane Structure and Behavior
- Monoclonal and Polyclonal Antibodies Research
- Advanced NMR Techniques and Applications
- Food Chemistry and Fat Analysis
- Endoplasmic Reticulum Stress and Disease
- Drug Transport and Resistance Mechanisms
- Alzheimer's disease research and treatments
- Iron Metabolism and Disorders
- Bacterial Genetics and Biotechnology
- RNA modifications and cancer
- Hepatitis C virus research
- Transgenic Plants and Applications
University of Sannio
2015-2025
Philipps University of Marburg
2023
Goethe University Frankfurt
2015-2017
University of Naples Federico II
1997-2016
European Molecular Biology Organization
2006-2009
European Molecular Biology Laboratory
2006
Istituto di Chimica Biomolecolare
1999-2003
Medical Research Council
2002
Photolyases, a ubiquitous class of flavoproteins, use blue light to repair DNA photolesions. In this work, we determined the structural mechanism photolyase-catalyzed cyclobutane pyrimidine dimer (CPD) lesion using time-resolved serial femtosecond crystallography (TR-SFX). We obtained 18 snapshots that show time-dependent changes in four reaction loci. used these results create movie depicts CPD lesions picosecond-to-nanosecond range, followed by recovery enzymatic moieties involved...
Report28 April 2009Open Access Efficient protein depletion by genetically controlled deprotection of a dormant N-degron Christof Taxis EMBL, Cell Biology and Biophysics Unit, Meyerhofstr. 1, Heidelberg, GermanyPresent address: Philipps-Universität Marburg, Biologie—Genetik, Karl-von-Friesch-Str. 8, 35043 Germany Search for more papers this author Gunter Stier Max-Planck Institute Medical Research, Jahnstrasse 29, 69120 Roberta Spadaccini Universita′ degli studi del Sannio, Department...
The p14 subunit of the essential splicing factor 3b (SF3b) can be cross-linked to branch-point adenosine pre-mRNA introns within spliceosome. stably interacts with SF3b SF3b155, which also binds 65-kDa U2 auxiliary (U2AF65). We combined biochemical and NMR techniques study conformation either alone or complexed SF3b155 fragments, as well an interaction network involving p14, U2AF65, snRNA/pre-mRNA. comprises a canonical RNA recognition motif (RRM) additional C-terminal helix (αC) β hairpin...
Knowledge of the structural properties allergenic proteins is a necessary prerequisite to better understand molecular bases their action, and also design targeted structural/functional modifications. Peamaclein recently identified 7 kDa peach allergen that has been associated with severe allergic reactions in sensitive subjects. This protein represents first component new family, which no 3D structure available yet. Here, we report experimental data on 3D-structure Peamaclein. Almost 75%...
Bovine seminal (BS) RNase, the unique natively dimeric member of RNase super-family, represents a special case not only for its additional biological actions but also singular features 3D domain swapping. The native enzyme is indeed mixture two isoforms: M = M, dimer held together by inter-subunit disulfide bonds, and MxM, 70% total, which, besides mentioned disulfides, additionally stabilized swapping N-termini.When lyophilized from 40% acetic acid, BS-RNase oligomerizes as super-family...
Site-specific proteases are important tools for in vitro and vivo cleavage of proteins. They widely used diverse applications, like protein purification, assessment protein–protein interactions or regulation localization, abundance activity. Here, we report the development a procedure to select protease variants with altered specificity based on well-established Saccharomyces cerevisiae adenine auxotrophy-dependent red/white colony assay. We applied this method tobacco etch virus (TEV)...
Three-dimensional domain swapping is a common phenomenon in pancreatic-like ribonucleases. In the aggregated state, these proteins acquire new biological functions, including selective cytotoxicity against tumour cells. RNase A able to dislocate both N- and C-termini, but usually this process requires denaturing conditions. contrast, bovine seminal ribonuclease (BS-RNase), which homo-dimeric protein sharing 80% of sequence identity with A, occurs natively as mixture swapped unswapped...
Fungal hydrophobins are amphipathic, highly surface-active, and self-assembling proteins. The class I hydrophobin Vmh2 from the basidiomycete fungus Pleurotus ostreatus seems to be most hydrophobic characterized so far. Structural functional properties of protein as a function environmental conditions have been determined. At least three distinct phenomena can occur, being modulated by conditions: (1) when pH increases or in presence Ca(2+) ions, an assembled state, β-sheet rich, is formed;...
The design of safe sweeteners is very important for people who are affected by diabetes, hyperlipemia, and caries other diseases that linked to the consumption sugars. Sweet proteins, which found in several tropical plants, many times sweeter than sucrose on a molar basis. A good understanding their structure-function relationship can complement traditional SAR studies small molecular weight thus help sweeteners. However, there virtually no sequence homology little structural similarity...
Abstract ATP binding cassette (ABC) transporters form a superfamily of integral membrane proteins involved in translocation substrates across the driven by hydrolysis. Despite available crystal structures and extensive biochemical data, many open questions regarding their transport mechanisms remain. Therefore, there is need to explore spectroscopic techniques such as solid state NMR order bridge gap between structural mechanistic data. In this study, we investigate feasibility using...
Protein quality control (PQC) mechanisms are essential for degradation of misfolded or dysfunctional proteins. An part protein homeostasis is recognition defective proteins by PQC components and their elimination the ubiquitin–proteasome system, often concentrating on termini as indicators integrity. Changes in amino acid composition C-terminal ends arise through disintegration, alternative splicing, during translation step synthesis from premature termination translational stop-codon...
Abstract The original structure of bovine seminal ribonuclease (BS‐RNase), solved in 1993, represents a milestone the story protein structure, because it represented first X‐ray showing two polypeptide chains entangled through their terminal regions. It is generally assumed that this structural feature basis several special biological activities, including potent antitumor activity, but has not been yet definitely proved. To assess hypothesis, article we have analyzed effects N‐terminal...