A5062281069- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Photoreceptor and optogenetics research
- Legume Nitrogen Fixing Symbiosis
- Indoor Air Quality and Microbial Exposure
- Environmental DNA in Biodiversity Studies
- Molecular Communication and Nanonetworks
- Microbial infections and disease research
- Air Quality and Health Impacts
- Protist diversity and phylogeny
- Plant Virus Research Studies
- Neuroscience and Neuropharmacology Research
- Plant nutrient uptake and metabolism
- Amino Acid Enzymes and Metabolism
- Species Distribution and Climate Change
- Soil Carbon and Nitrogen Dynamics
- Nematode management and characterization studies
- Antimicrobial agents and applications
- Probiotics and Fermented Foods
- Ammonia Synthesis and Nitrogen Reduction
- Plant Pathogens and Fungal Diseases
- bioluminescence and chemiluminescence research
- Nicotinic Acetylcholine Receptors Study
- Plant Pathogenic Bacteria Studies
University of Alicante
2021-2025
Agència de Qualitat i Avaluació Sanitàries de Catalunya
2025
Royal Netherlands Institute for Sea Research
2019-2022
University of Padua
2013-2021
Universitat de Miguel Hernández d'Elx
2016-2020
Utrecht University
2020
Moscow Institute of Physics and Technology
2020
Abstract Microbes drive ecosystems under constraints imposed by viruses. However, a lack of virus genome information hinders our ability to answer fundamental, biological questions concerning microbial communities. Here we apply single-virus genomics (SVGs) assess whether portions marine viral communities are missed current techniques. The majority the here-identified 44 single-amplified genomes (vSAGs) more abundant in global ocean virome data sets than published metagenome-assembled or...
ABSTRACT We present a metagenomic study of Lake Baikal (East Siberia). Two samples obtained from the water column under ice cover (5 and 20 m deep) in March 2016 have been deep sequenced reads assembled to generate metagenome-assembled genomes (MAGs) that are representative microbes living this special environment. Compared with freshwater bodies studied around world, had an unusually high fraction Verrucomicrobia . Other groups, such as Actinobacteria Proteobacteria , were proportions...
Summary Members of the SAR11 clade, despite their high abundance, are often poorly represented by metagenome‐assembled genomes. This fact has hampered our knowledge about ecology and genetic diversity. Here we examined 175 genomes, including 47 new single‐amplified The presence first genomes associated with subclade IV suggests that, in same way as V, they might be outside proposed Pelagibacterales order. An expanded phylogenomic classification together patterns metagenomic recruitment at a...
Abstract The analysis of environmental microbial communities has largely relied on a PCR-dependent amplification genes entailing species identity as 16S rRNA. This approach is susceptible to biases depending the level primer matching in different species. Moreover, possible yet-to-discover taxa whose rRNA could differ enough from known ones would not be revealed. DNA-based methods moreover do provide information actual physiological relevance each taxon within an environment and are affected...
Abstract Recently, two groups of rhodopsin genes were identified in large double-stranded DNA viruses. The structure and function viral rhodopsins are unknown. We present functional characterization high-resolution an Organic Lake Phycodnavirus II (OLPVRII) group 2. It forms a pentamer, with symmetrical, bottle-like central channel the narrow vestibule cytoplasmic part covered by ring 5 arginines, whereas phenylalanines form hydrophobic barrier its exit. proton donor E42 is placed helix B....
Abstract Background Lake Baikal is the largest body of liquid freshwater on Earth. Previous studies have described microbial composition this habitat, but viral communities from ecosystem not been characterized in detail. Results Here, we describe diversity habitat across depth and seasonal gradients. We discovered 19,475 bona fide sequences, which are derived viruses predicted to infect abundant ecologically important taxa that reside Baikal, such as Nitrospirota, Methylophilaceae,...
Phytoplankton is the base of marine food chain as well oxygen and carbon cycles thus plays a global role in climate ecology. Nucleocytoplasmic Large DNA Viruses that infect phytoplankton organisms regulate dynamics encompass genes rhodopsins two distinct families. Here, we present functional structural characterization proteins viral rhodopsin group 1, OLPVR1 VirChR1. Functional analysis VirChR1 shows it highly selective, Na+/K+-conducting channel and, contrast to known cation...
The long-term fate of plastics in the ocean and their interactions with marine microorganisms remain poorly understood. In particular, role sinking plastic particles as a transport vector for surface microbes towards deep sea has not been investigated. Here, we present first data on composition microbial communities floating suspended recovered from to bathypelagic water column (0-2000 m depth) North Pacific Subtropical Gyre. Microbial community differed that afloat at surface. However, both...
Abstract Dust particles lifting and discharge from Africa to Europe is a recurring phenomenon linked air circulation conditions. The possibility that microorganisms are conveyed across distances entails important consequences in terms of biosafety pathogens spread. Using culture independent DNA-based analyses via next generation sequencing the 16 S genes airborne metagenome, atmospheric microbial community was characterized hypothesis tested shifts species diversity could be recorded...
Summary We present two genomes of widespread freshwater picocyanobacteria isolated by extinction dilution from a Spanish oligotrophic reservoir. Based on microscopy and genomic properties, both were tentatively designated Synechococcus lacustris Tous, formerly described as metagenome assembled genome (MAG) the same habitat, Cyanobium usitatum here for first time. Both strains purified in unicyanobacterial cultures, their sequenced. They are broadly distributed systems; seems to be specialist...
The evolutionary interactions between viruses and their prokaryotic hosts remain a little-known aspect of microbial evolution. Most studies on this topic were carried out in pure cultures that challenge one virus with bacterial clone at time, which is very removed from real-life situations. Few have addressed trends microdiversity marine viral communities throughout depth gradients. We analyzed metagenomes both the cellular fractions Mediterranean seawater samples spanning epipelagic to...
The host recognition modules encoding the injection machinery and receptor binding proteins (RBPs) of bacteriophages are predisposed to mutation recombination maintain infectivity towards co-evolving bacterial hosts. In this study, we reveal how Alteromonas mediterranea schitovirus A5 shares its module, including tail fiber cognate chaperone, with phages from distantly related families myovirus V22. While V22 chaperone is essential for producing active fibers, here demonstrate production...
Host recognition by phage-encoded receptor binding proteins (RBPs) constitutes the first step in all phage infections and most critical determinant of host specificity. By characterizing new types RBPs identifying their essential chaperones, we hope to expand repertoire known phage-host machineries. Due genetic plasticity, studying associated chaperones can shed light onto viral evolution affecting interactions, which is for fields such as therapy or biotechnology. In addition, since marine...
The prominent feature of rhizobia is their molecular dialogue with plant hosts. Such interaction enabled by the presence a series symbiotic genes encoding for synthesis and export signals triggering organogenetic physiological responses in plant. genome Rhizobium sullae type strain IS123T nodulating legume Hedysarum coronarium, was sequenced resulted 317 scaffolds total assembled size 7,889,576 bp. Its features were compared those genomes from representing an increasing gradient taxonomical...
Haloquadratum walsbyi dominates saturated thalassic lakes worldwide where they can constitute up to 80-90% of the total prokaryotic community. Despite abundance enigmatic square-flattened cells, only 7 isolates are currently known with 2 genomes fully sequenced and annotated due difficulties grow them under laboratory conditions. We have performed a transcriptomic analysis one these isolates, Spanish strain HBSQ001 in order investigate gene transcription light dark conditions.Despite...
Pangenome analyses reveal major clues on evolutionary instances and critical genome core conservation. The order Rhizobiales encompasses several families with rather disparate ecological attitudes. Among them, Rhizobiaceae, Bradyrhizobiaceae, Phyllobacteriacreae Xanthobacteriaceae, include members proficient in mutualistic symbioses plants based the bacterial conversion of N2 into ammonia (nitrogen-fixation). pangenome 12 nitrogen-fixing plant symbionts was analyzed yielding total 37,364...
Abstract Phytoplankton is the base of marine food chain, oxygen, carbon cycle playing a global role in climate and ecology. Nucleocytoplasmic Large DNA Viruses regulating dynamics phytoplankton comprise genes rhodopsins two distinct families. We present function-structure characterization homologous proteins representatives family 1 viral rhodopsins, OLPVR1 VirChR1. VirChR1 highly selective, Ca 2+ -dependent, Na + /K - conducting channel and, contrast to known cation channelrhodopsins...
Assessing the response of microbial communities to nutrient inputs in man-managed soils is primary importance understand impact on ecosystem services provided by soil microbiome. In this study, a low-nutrient was supplemented with seven different innovative fertilizers including matrixes plant, animal, fungal or synthetic origin, and dosed deliver same amount nitrogen. Growth potted grass crop (Cynodon dactylon) recorded were scored plant yield obtained greenhouse study. Soil sampled at 9 58...
Abstract Transposable elements (TEs) play a pivotal role in the evolution of genomes across all life domains. ‘Miniature Inverted-repeat Transposable-Elements’ (MITEs) are non-autonomous TEs mainly located intergenic regions, relying on external transposases for mobilization. The extent MITEs’ mobilome was explored nearly 1700 prokaryotic genera, 183 232 genomes, revealing broad distribution. MITEs were identified 56.5% totaling over 1.4 million cMITEs (cellular MITEs). Cluster analysis...
Solar crystallizer ponds are characterized by high population density with a relatively simple community structure in terms of species composition. The microbial the solar saltern Santa Pola (Alicante, Spain), is largely dominated hyperhalophilic square archaeon Haloquadratum walsbyi . Here we studied metatranscriptomes retrieved from pond during winter 2012 and summer 2014 compared Hqr. walsbyi’s transcription patterns that cultured strain HBSQ001. Significant differences were found between...