A5055048487- Plant Reproductive Biology
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Photosynthetic Processes and Mechanisms
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant biochemistry and biosynthesis
Huazhong Agricultural University
2013-2022
Shanghai Zhangjiang Laboratory
2022
Meiosis is a fundamental process for sexual reproduction in most eukaryotes and the evolutionarily conserved recombinases RADiation sensitive51 (RAD51) Disrupted Meiotic cDNA1 (DMC1) are essential meiosis thus fertility. The mitotic function of RAD51 clear, but meiotic remains largely unknown. Here we show that functions as an interacting protein to restrain Structural Maintenance Chromosomes5/6 (SMC5/6) complex from inhibiting DMC1. We unexpectedly found loss SMC5/6 partially suppresses...
Homologous recombination repair (HR) is an error-free DNA damage pathway to maintain genome stability and a basis of gene targeting using genome-editing tools. However, the mechanisms HR in plants are still poorly understood. Through genetic screens for response mutants (DDRM) Arabidopsis, we find that plant-specific ubiquitin E3 ligase DDRM1 required HR. contains N-terminal BRCT (BRCA1 C-terminal) domain C-terminal RING (really interesting new gene) highly conserved including mosses. The...
Small peptides secreted to the extracellular matrix control many aspects of plant's physiological activities which were identified in Arabidopsis thaliana, called ATSPs. Here, we isolated and characterized small peptide gene Bna.SP6 from Brassica napus. The BnaC.SP6 promoter was cloned identified. Promoter deletion analysis suggested that -447 -375 -210 -135 regions are crucial for silique septum pollen expression BnaC.SP6, respectively. Furthermore, minimal region p158 (-210 -52) sufficient...
Bioinformation showed that BnRabGDI3 gene promoter included a 2 064bp-long segment at the upstream translation start site in Brassica napus genome.Promoter sequence contained several anther specific cis-elements.To verify biology function,a recombinant vector was designated as pBnRabGDI3:GUS through replacement of CaMV35S pBI121 by cloned fragment.In vector,gus reporter driven promoter.GUS histochemical assay six independent transgenic plants gus expressed only anthers from 11th to 13th...