A5055938871- Genomics and Chromatin Dynamics
- Genetics and Neurodevelopmental Disorders
- Cardiac, Anesthesia and Surgical Outcomes
- RNA Research and Splicing
- Global Health Care Issues
- Hip and Femur Fractures
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Hematological disorders and diagnostics
- Retinal Development and Disorders
- Electrolyte and hormonal disorders
- Epigenetics and DNA Methylation
- Muscle and Compartmental Disorders
- Multiple and Secondary Primary Cancers
- Hemoglobinopathies and Related Disorders
- Clinical Nutrition and Gastroenterology
- Medical Practices and Rehabilitation
- Palliative Care and End-of-Life Issues
- Frailty in Older Adults
- Diagnosis and Treatment of Venous Diseases
- Cardiac Imaging and Diagnostics
- Neurological diseases and metabolism
- Respiratory Support and Mechanisms
- Single-cell and spatial transcriptomics
- Health, Medicine and Society
University of California, San Francisco
2014-2024
Stony Brook University Hospital
2023-2024
Langley Porter Psychiatric Hospital and Clinics
2021-2022
University of Oxford
2000-2019
Broad Institute
2014
Broad Center
2014
Biologie du Développement et Cellules Souches
2014
Neurological Surgery
2014
NeuroDevelopment Center
2014
John Radcliffe Hospital
2001-2013
DLX transcription factors (TFs) are master regulators of the developing vertebrate brain, driving forebrain GABAergic neuronal differentiation. Ablation Dlx1&2 alters expression genes that critical for development. We integrated epigenomic and transcriptomic analyses, complemented with in situ hybridization (ISH), vivo vitro studies regulatory element (RE) function. This revealed DLX-organized gene network at genomic, cellular, spatial levels mouse embryonic basal ganglia. TFs perform dual...
The postnatal functions of the Dlx1&2 transcription factors in cortical interneurons (CINs) are unknown. Here, using conditional Dlx1, Dlx2, and knockouts (CKOs), we defined their roles specific CINs. CKOs had dendritic, synaptic, survival defects, affecting even PV+ We provide evidence that DLX2 directly drives Gad1, Gad2, Vgat expression, show mutants reduced mIPSC amplitude. In addition, formed fewer GABAergic synapses on excitatory neurons frequency. Furthermore, Dlx1/2 CKO hypoplastic...
Deleterious genetic variants in POGZ, which encodes the chromatin regulator Pogo Transposable Element with ZNF Domain protein, are strongly associated autism spectrum disorder (ASD). Although it is a high-confidence ASD risk gene, neurodevelopmental functions of POGZ remain unclear. Here we reveal genomic binding developing forebrain at euchromatic loci and gene regulatory elements (REs). We profile accessibility expression Pogz
The Otx2 homeodomain transcription factor is essential for gastrulation and early neural development. We generated conditional knockout (cKO) mice to investigate its roles in telencephalon development after neurulation (approximately embryonic day 9.0). conducted transcriptional profiling situ hybridization identify genes de-regulated cKO ventral forebrain. In parallel, we used chromatin immunoprecipitation sequencing enhancer elements, the OTX2 binding motif, that are likely direct targets...
The development of high-oleic palm oil alternatives through microbial fermentation offers a sustainable solution to the environmental challenges associated with traditional cultivation. In this study, Prototheca moriformis microalgae strain was optimized via classical improvement techniques produce fatty acid and triacylglycerol (TAG) profile similar conventional oil. Iterative rounds mutagenesis screening enhanced palmitic content from 28 30-32% oleic 60 55-57% total acids, an yield 136.5...
The development of high-oleic palm oil alternatives through microbial fermentation offers a sustainable solution to the environmental challenges associated with traditional cultivation. In this study, Prototheca moriformis microalgae strain was optimized via classical improvement techniques produce fatty acid and triacylglycerol (TAG) profiles similar those conventional oil. Iterative rounds mutagenesis screening enhanced palmitic content from 28 30–32% oleic 60 55–57% total acids, an yield...
ABSTRACT In the developing subpallium, fate decision between neurons and glia is driven by expression of Dlx1/2 or Olig1/2, respectively, two sets transcription factors with a mutually repressive relationship. The mechanism which repress progenitor oligodendrocyte fate, while promoting genes needed for differentiation, not fully understood. We identified motif within DLX1 that binds RBBP4, NuRD complex subunit. ChIP-seq studies genomic occupancy six different members show colocalize to...
Mixed lineage leukemia-1 (Mll1) epigenetically regulates gene expression patterns that specify cellular identity in both embryonic development and adult stem cell populations. In the mouse brain, multipotent neural cells (NSCs) subventricular zone generate new neurons throughout life, Mll1 is required for this postnatal neurogenesis but not glial differentiation. Analysis of Mll1-dependent transcription may identify neurogenic genes useful direct reprogramming astrocytes into neurons.To...
Non-neurogenic cell types, such as cortical astroglia and fibroblasts, can be directly converted into neurons by the overexpression of defined transcription factors. Normally, cellular phenotype differentiated cells is remarkably stable resists direct transdifferentiation. Here we show that Ink4a/Arf (also known Cdkn2a ) locus a developmental barrier to neuronal transdifferentiation induced factor overexpression. With serial passage in vitro , wild-type postnatal become progressively...
ABSTRACT Transcription factors (TFs) bind combinatorially to genomic cis-regulatory elements (cREs), orchestrating transcription programs. While studies of chromatin state and chromosomal interactions have revealed dynamic neurodevelopmental cRE landscapes, parallel understanding the underlying TF binding lags. To elucidate combinatorial TF-cRE driving mouse basal ganglia development, we integrated ChIP-seq for twelve TFs, H3K4me3-associated enhancer-promoter interactions, transcriptional...
Introduction: a professional man sustained residual, persisting, isolated impairment of short‐term memory secondary to severe carbon monoxide poisoning. Informal, open trial the cholinesterase inhibitor donepezil resulted in uncertain benefit.