A5023478427- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Complement system in diseases
- Monoclonal and Polyclonal Antibodies Research
- Phytochemicals and Antioxidant Activities
- Essential Oils and Antimicrobial Activity
- Bee Products Chemical Analysis
- Systemic Lupus Erythematosus Research
- Cell Adhesion Molecules Research
- Blood groups and transfusion
- Phytochemistry Medicinal Plant Applications
- Atherosclerosis and Cardiovascular Diseases
- Natural product bioactivities and synthesis
- Sesquiterpenes and Asteraceae Studies
- Flavonoids in Medical Research
- Galectins and Cancer Biology
- Bioactive Compounds and Antitumor Agents
- Blood disorders and treatments
- bioluminescence and chemiluminescence research
- Fungal Infections and Studies
- Erythrocyte Function and Pathophysiology
- Nitric Oxide and Endothelin Effects
- S100 Proteins and Annexins
- Antioxidant Activity and Oxidative Stress
- Parasitic Infections and Diagnostics
Universidade de São Paulo
2011-2020
Universidade de Ribeirão Preto
2005-2020
SUPERA Park of Innovation and Technology of Ribeirão Preto
1999-2010
Universidade Brasil
2009
Laboratoire de Biochimie
2003
Medical Research Council
1991
SUMMARY A systematic study has been carried out to investigate the role of immunoglobulin isotype, epitope density, and antigen/antibody ratio on capacity immune complexes activate classical alternative pathways human complement for subsequently bind erythrocyte C3b-C4b receptors (CR1). For this purpose, a series chimaeric monoclonal anti-NIP antibodies was used, which all shared same combining site but had different constant domains. Antigen density varied by coupling numbers NIP hapten...
Testosterone exerts both beneficial and harmful effects on the cardiovascular system. Considering that testosterone induces reactive oxygen species (ROS) generation ROS activate cell death signaling pathways, we tested hypothesis apoptosis in vascular smooth muscle cells (VSMCs) via mitochondria-dependent generation. Potential mechanisms were addressed. Cultured VSMCs stimulated with (10(-7) mol/l) or vehicle (2-12 h) presence of flutamide (10(-5) mol/l), CCCP (10(-6) mimetic manganese(III)...
Pathogenesis of chronic inflammatory diseases is associated with excessive elastase release through neutrophil degranulation. In the present study, inhibition human degranulation by four flavonoids (myricetin, quercetin, kaempferol, galangin) was evaluated using released as a biomarker. Inhibitory potency observed in following order: quercetin > myricetin kaempferol = galangin. Quercetin, most potent inhibitor also had weak inhibitory effect on enzyme catalytic activity. Furthermore,...
This study investigated the potential use of topically and orally administered propolis extracts to prevent UV irradiation-induced oxidative stress in skin. The results illustrated that green extract (GPE) contained greater amounts polyphenols, coumaric acid, drupanin, baccharin artepillin C than did brown (BPE). GPE showed higher antioxidant activity BPE when IC(50) (concentration caused 50% inhibition) values were compared. Interesting, oral treatment hairless mice demonstrated a recovery...
We have investigated the individual role of FcgR and CR, as well their cooperation, in mediating oxidative burst degranulation neutrophils Brazilian systemic lupus erythematosus (SLE) patients. Neutrophils were stimulated with immune complexes (IC)-IgG or-F(ab')2, opsonized or not normal SLE human serum. The was decreased active patients compared to healthy controls when this response mediated by and/or while unaffected. hypocomplementemia did affect only CR. FcgRII CR1 expression on...
The Brazilian medicinal plant Lychnophora ericoides is commercially available as an analgesic and anti-inflammatory agent. phytochemical investigation of the leaf polar extract yielded 6,8-di-C-β-glucosylapigenin (1) new compound 6,8-di-C-β-glucosylchrysin (2). 6,8-Di-C-β-glucosylapigenin showed significant activity in carrageenan-induced rat paw edema. We did not observe any statistical difference between two compounds (1 2) inhibiting chemiluminescence opsonized zymosan-stimulated...
Context and objective: The massive production of reactive oxygen species by neutrophils during inflammation may cause damage to tissues. Flavonoids act as antioxidants have anti-inflammatory effects. In this study, liposomes loaded with these compounds were evaluated potential antioxidant carriers, in attempt overcome their poor solubility stability. Materials methods: Liposomes containing quercetin, myricetin, kaempferol or galangin prepared the ethanol injection method analyzed inhibitors...
A new tendency in cosmetic formulations is the association of botanical extracts and vitamins to improve skin conditions by synergic effects. The objective this study was determine antioxidant activity associated bioflavonoids, retinyl palmitate (RP), tocopheryl acetate (TA) ascorbyl tetraisopalmitate (ATIP), as well their photoprotective effects preventing increased erythema, transepidermal water loss (TEWL) sunburn cell formation hairless mouse skin. solutions containing or each substance...
Flavonoids have been reported to exhibit several pharmacological properties, mainly in cardiovascular and inflammatory diseases. In the present study, we observed that rutin, a known glycosylated flavonoid isolated from Dimorphandra mollis, had lowering effect on plasma triglyceride levels of diet-induced hypercholesterolemic Golden Syrian hamsters, but did not change total cholesterol high-density lipoprotein levels. Moreover, high-fat or rutin supplemented diets showed no immunotoxic...
Liposomes have been employed as potential drug carriers. However, after their in vivo administration, they can be destabilized by proteins of complement system, contributing to the clearance vesicles from blood circulation. Antioxidant flavonoids such quercetin reported beneficial human health, but low water solubility and bioavailability limit enteric administration. Therefore, development appropriate flavonoid-carriers could great importance therapy. The aim present study was evaluate...
This paper describes the antioxidant effects of thirteen phenolic compounds isolated from plants genus Lychnophora. Two assays were performed to evaluate these effects: a cellular test that measured luminol-enhanced chemiluminescence produced by neutrophils stimulated with opsonized zymosan and cell-free involving horseradish peroxidase-H2O2-luminol. In both assays, activity was dependent on their concentration chemical structure. Our results suggest ability Lychnophora species scavenge...
Toxoplasma gondii stimulates a potent pro-inflammatory response and neutrophils are involved in early infection. Galectin-3 (Gal-3) is an endogenous modulator of inflammatory processes anti-infective agents, but its interaction with T. infection still unclear. Here, we evaluated the role Gal-3 peritoneal inflammation, reactive oxygen species (ROS) production by survival, after vivo virulent RH strain, using deficient wild type mice. Animals were inoculated thioglycollate or tachyzoites,...