A5030940089- Cellular Mechanics and Interactions
- Elasticity and Material Modeling
- Force Microscopy Techniques and Applications
- Automotive and Human Injury Biomechanics
- Neurogenesis and neuroplasticity mechanisms
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
- Neuroinflammation and Neurodegeneration Mechanisms
- Axon Guidance and Neuronal Signaling
- Erythrocyte Function and Pathophysiology
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Mechanical and Optical Resonators
- Nicotinic Acetylcholine Receptors Study
- Nerve injury and regeneration
- Memory and Neural Mechanisms
German Cancer Research Center
2017-2018
Heidelberg University
2017-2018
University Hospital Heidelberg
2017-2018
University of Cambridge
2015-2018
University of Cologne
2015-2018
Abstract Injury to the central nervous system (CNS) alters molecular and cellular composition of neural tissue leads glial scarring, which inhibits regrowth damaged axons. Mammalian scars supposedly form a chemical mechanical barrier neuronal regeneration. While tremendous effort has been devoted identifying characteristics scar, very little is known about its properties. Here we characterize spatiotemporal changes elastic stiffness injured rat neocortex spinal cord at 1.5 three weeks...
Mechanical signaling plays an important role in cell physiology and pathology. Many types, including neurons glial cells, respond to the mechanical properties of their environment. Yet, for spinal cord tissue, data on tissue stiffness are sparse. To investigate regional direction-dependent at a spatial resolution relevant individual we conducted atomic force microscopy (AFM) indentation tensile measurements acutely isolated mouse sectioned along three major anatomical planes, correlated...
Microglial cells are key players in the primary immune response of central nervous system. They highly active and motile that chemically mechanically interact with their environment. While impact chemical signaling on microglia function has been studied much detail, current understanding mechanical is very limited. When cultured compliant substrates, microglial adapted spread area, morphology, actin cytoskeleton to stiffness Traction force microscopy revealed forces exerted by increase...
Highlights•Long-range GABAergic projections from the motor cortex directly innervate striatum•M1 and M2 long-range SOM+ PV+ neurons differentially striatal neurons•Striatal cholinergic are innervated mainly by M1 projecting neurons•Motor modulate locomotionSummaryThe cortico-basal ganglion loop is critical for planning, execution, learning. Balanced excitation inhibition in this crucial proper output. Excitatory have been thought to be only source of cortical input striatum. Here, we...
Significance Acetylcholine is a key modulator of hippocampal and entorhinal cortex (EC) function. The majority cholinergic projections targeting these structures originate in the basal forebrain complex, specifically medial septum. Many studies focused on behavioral effects involving projections, but there still paucity regarding their connectivity target area. Here we provide this missing link. By combining optogenetics with whole-cell recordings superficial EC layers, identified synaptic...
Abstract Local tissue stiffness provides an important signal to which cells respond in vivo. However, assessing mechanics is currently challenging and requires sophisticated technology. We here developed a model quantitatively predicting nervous heterogeneities at cellular resolution based on cell density, myelin GFAP fluorescence intensities. These histological parameters were identified by correlation analysis of atomic force microscopy-based elasticity maps spinal cord sections...