We report the first experimental demonstration of two-photon imaging with a pseudothermal source. Similarly to case entangled states, Gaussian thin lens equation is observed, indicating EPR type correlation in position. introduce concepts coherent and incoherent imaging. The differences between thermal cases are explained terms these concepts.

The mechanical properties of corneal tissue are linked to prevalent ocular diseases and therapeutic procedures. Brillouin microscopy is a novel optical technology that enables three-dimensional imaging. In this study, the feasibility noncontact technique was tested for in situ quantitative assessment biomechanical cornea.Brillouin light-scattering involves spectral shift proportional longitudinal modulus elasticity tissue. A 532-nm single-frequency laser custom-developed ultrahigh-resolution...

Corneal collagen crosslinking (CXL) is designed to halt the progression of keratoconus and corneal ectasia by inducing stiffening. However, it currently difficult monitor evaluate CXL outcome objectively due lack suitable methods characterize mechanical properties. We validated noncontact Brillouin microscopy quantify properties before after CXL.CXL was performed on fresh porcine eyes using various presoaking times light doses, with or without epithelial debridement. From maps elastic...

Loss of corneal strength is a central feature keratoconus progression. However, it currently difficult to measure mechanical changes noninvasively. The objective this study evaluate if Brillouin optical microscopy can differentiate the properties keratoconic corneas versus healthy ex vivo.We obtained eight tissue samples from donor used in Descemet's stripping endothelial keratoplasty (DSEK) and 10 advanced patients undergoing deep anterior lamellar (DALK). Within 2 hours after surgery,...

Abstract Advances in photonics have stimulated significant progress medicine, with many techniques now routine clinical use. However, the finite depth of light penetration tissue is a serious constraint to utility. Here we show implantable light-delivery devices made bio-derived or biocompatible, and biodegradable polymers. In contrast conventional optical fibres, which must be removed from body soon after use, biocompatible waveguides may used for long-term delivery need not as they are...

Purpose To determine the relationship between regional epithelial thickness and corneal toricity estimate epithelium's impact on power in normal corneas. Methods This was a retrospective case-control study evaluating 200 eyes from patients categorized into one of three groups based obtained with Scheimpflug imaging: (1) 80 non-toric (NT) (< 0.50 diopters [D] any meridian), (2) with-the-rule (WTR) (⩾ +1.50 D @90 ± 22.5°), (3) 40 against-the-rule (ATR) @180 22.5°). Epithelial maps (9 mm)...

Two-photon correlation phenomena, including the historical experiment of Hanbury Brown and Twiss, may have to be described quantum mechanically, regardless whether source radiation is classical or quantum. Supporting this point, we present a ghost imaging type second-order spatial chaotic light show that understanding based on concept statistical intensity fluctuations does not give correct interpretation for observation. From practical point view, demonstrates possibility having high...

We report the first Brillouin measurement of human eye in vivo.We constructed a optical scanner safe for use by employing continuous-wave laser light at 780 nm low power 0.7 mW.With single scan along optic axis eye, axial profile frequency shift was obtained with pixel acquisition time 0.4 s and resolution about 60 µm, showing depth-dependent biomechanical properties cornea lens.

Fluorescent nanosized carbon dots (Cdots) are an emerging bioimaging agent with excellent chemical inertness and marginal cytotoxicity in comparison to widely used semiconductor quantum dots. In this work, we report the application of Cdots for real time target specific delivery hyaluronic acid (HA) derivatives. Polyethylene glycol (PEG) diamine-capped were synthesized by pyrolysis citric a hot solvent. The showed strong fluorescence under UV excitation emission properties dependending on...

Purpose.: Photochemical cross-linking of corneal collagen is an evolving treatment for keratoconus and other ectatic disorders. We evaluated by rose bengal plus green light (RGX) in rabbit eyes investigated factors important clinical application. Methods.: Rose (RB, 0.1%) was applied to deepithelialized corneas enucleated 2 minutes. The diffusion distance RB into the stroma measured fluorescence microscopy on frozen sections. RB-stained were exposed (532-nm) 3.3 9.9 minutes (50–150 J/cm2)....

We demonstrate a high-resolution high-extinction parallel spectrometer for Brillouin spectroscopy of turbid samples. Cascading multiple VIPA etalons in the cross-axis configuration allowed us to achieve high extinction ratio up 80 dB with sub-GHz resolution. Using three-stage VIPA, we obtained spectra from Intralipid solutions at concentrations 10%.

High-resolution imaging through turbid media is a fundamental challenge of optical sciences that has attracted lot attention in recent years for its wide range potential applications. Here, we demonstrate the resolution systems looking behind highly scattering medium can be improved below diffraction-limit. To achieve this, novel microscopy technique enabled by memory effect uses deconvolution image processing and thus it does not require iterative focusing, scanning or phase retrieval...

Corneal mechanical strength is critical to withstanding intraocular pressure and maintaining normal shape1,2. In keratoconus, the stability compromised3, this may lead progressive morphological changes. Therefore, a noninvasive technique capable of accurately measuring properties cornea help understand mechanism keratoconus development improve detection intervention in keratoconus. We have previously developed Brillouin microscopy based on light scattering from inherent acoustic waves...

Microglial cells are key players in the primary immune response of central nervous system. They highly active and motile that chemically mechanically interact with their environment. While impact chemical signaling on microglia function has been studied much detail, current understanding mechanical is very limited. When cultured compliant substrates, microglial adapted spread area, morphology, actin cytoskeleton to stiffness Traction force microscopy revealed forces exerted by increase...

Several phenomena have been recently exploited to circumvent scattering and succeeded in imaging or focusing light through turbid layers. However, the requirement for medium be steady during process remains a fundamental limitation of these methods. Here we introduce an optical modality that overcomes this challenge by taking advantage so-called shower-curtain effect, adapted spatial-frequency domain via speckle correlography. We present high resolution objects hidden behind millimeter-thick...

Simple plant cell morphologies, such as cylindrical shoot cells, are determined by the extensibility pattern of primary wall, which is thought to be largely dominated cellulose microfibrils, but mechanism leading more complex shapes, interdigitated patterns in epidermis many eudicotyledon leaves, much less well understood. Details about manner wall polymers at periclinal regulate morphogenetic process epidermal pavement cells and mechanistic information initial steps characteristic...

Abstract The mechanical properties of the cellular nucleus are extensively studied as they play a critical role in important processes, such cell migration, gene transcription, and stem differentiation. While isolated have been tested, there is lack measurements about behavior within intact cells specifically interplay internal nuclear components with intracellular microenvironment, because current testing methods based on contact only allow studying after isolation from or disruption...