The prevalence of immune-related systemic manifestations associated with inflammatory bowel disease varies widely in different studies. This prospective hospital study examines the and clinical aspects these manifestations. A total 792 patients, 449 Crohn's 343 ulcerative colitis, were followed up from diagnosis to 20 years. Data related extent severity intestinal lesions obtained at during follow-up. Extraintestinal activity inflammation appeared least once 25.8% patients. cumulative...

Prostate cancer (PCa), a highly incident and heterogeneous malignancy, mostly affects men from developed countries. Increased knowledge of the biological mechanisms underlying PCa onset progression are critical for improved clinical management. MicroRNAs (miRNAs) deregulation is common in human cancers, understanding how it impacts major importance. MiRNAs downregulated cancer, although some overexpressed, playing role tumor initiation progression. We aimed to identify miRNAs overexpressed...

Prostate Cancer (PCa) overdiagnosis and overtreatment, as a consequence of the limited specificity current detection prognostication methods, remains major challenge in clinical practice. Therefore, development validation new molecular biomarkers amenable detecting clinically significant disease is crucial. MicroRNAs (miRNA) deregulation common cancer, constituting potential non-invasive for PCa prognostication. Herein, we evaluated screening prognostic biomarker two onco-microRNAs...

•Several anticancer therapies can cause IRRs minutes to hours after the infusion.•Prompt recognition and appropriate clinical assessment management of IRR are crucial for patient safety.•Lack standardization in prevention, management, reporting across institutions is an unmet need.•This article seeks help clinicians recognize early signs promptly manage event.•It also aims standardize procedures institutions. Several have potential infusion-related reactions (IRRs) form adverse events that...

Prostate cancer (PCa), a leading cause of cancer-related morbidity and mortality, arises through the acquisition genetic epigenetic alterations. Deregulation histone methyltransferases (HMTs) or demethylases (HDMs) has been associated with PCa development progression. However, precise influence altered HMTs HDMs expression respective marks in onset progression remains largely unknown. To clarify role prostate carcinogenesis, levels 37 20 were assessed normal tissue samples by RT-qPCR. SMYD3...

Disruption of microRNA (miRNA) expression patterns is now being recognized as a hallmark human cancer. The causes these altered profiles are diverse, and, among them, we found the existence defects in miRNA processing machinery. However, little known about how alterations affect biology underlying tumors. Herein, show that colorectal cancer cells with an impairment DICER1, major biogenesis gene, undergo enrichment tumor stemness features and epithelial-to-mesenchymal transition. These...

To characterize the pattern of ETS rearrangements and to uncover novel fusion genes, we analyzed 200 prostate carcinomas (PCa) with TaqMan low-density arrays (TLDAs), followed by selective analyses fluorescence in situ hybridization (FISH), RT-PCR, sequencing. Besides confirming recurrent presence ERG, ETV1, ETV4, ETV5 rearrangements, here report FLI1 as fifth transcription factor involved genes cancer. Outlier expression gene was detected TLDAs one PCa that showed relative overexpression...

Contemporary challenges of prostate cancer (PCa) include overdiagnosis and overtreatment, entailing the need for novel clinical tools to improve risk stratification therapy selection. PCa diagnosis prognostication might be perfected using epigenetic biomarkers, among which aberrant DNA methylation microRNA promoters has not been systematically explored. Herein, we identified aberrantly methylated microRNAs in assessed its diagnostic prognostic biomarker potential. Using HumanMethylation450...

Background: Current therapeutic strategies for advanced prostate cancer (PCa) are largely ineffective. Because aberrant DNA methylation associated with inappropriate gene-silencing is a common feature of PCa, inhibitors might constitute an alternative therapy. In this study we aimed to evaluate the anti-cancer properties RG108, novel non-nucleoside inhibitor methyltransferases (DNMT), in PCa cell lines. Keywords: Prostate cancer, methyltransferases, proliferation, apoptosis.

Numerous DNA-damaging cellular stresses, including oncogene activation and DNA-damage response (DDR), may lead to senescence. Previous observations linked microRNA deregulation with altered senescent patterns, prompting us investigate whether epigenetic repression of microRNAs expression might disrupt senescence in prostate cancer (PCa) cells.Differential methylation mapping tissues was carried using Infinium HumanMethylation450 BeadChip. After validation analyses a larger series tissues,...

Prostate cancer (PCa) is a major cause of morbidity and mortality. Until now the specific role histone methyltransferases (HMTs) deregulated expression/activity in PCa poorly understood. Herein we aimed to uncover potential oncogenic PRMT6 prostate carcinogenesis. overexpression was confirmed PCa, at transcript protein level. Stable knockdown PC-3 cells attenuated malignant phenotype, increasing apoptosis decreasing cell viability, migration invasion. silencing associated with decreased...

Prostate cancer (PCa) is a major cause of morbidity and mortality in men worldwide. MicroRNAs are globally downregulated PCa, especially poorly differentiated tumors. Nonetheless, the underlying mechanisms still elusive. Herein, using combined analysis microRNAs expression genomewide DNA methylation, we aimed to identify epigenetically PCa. We found that miR-152-3p was underexpressed PCa lower levels were associated with promoter hypermethylation accordance TCGA dataset analysis. Functional...

A large percentage of prostate cancers harbor TMPRSS2-ERG gene fusions, leading to aberrant overexpression the transcription factor ERG. The target genes deregulated by this rearrangement, however, remain mostly unknown. To address subject we performed genome-wide mRNA expression analysis on 6 non-malignant samples and 24 carcinomas with (n = 16) without 8) fusion as determined FISH. top-most differentially expressed their associations ERG over-expression were technically validated...

Constitutive activation of the Wnt signaling pathway is a common feature solid tumors and contributes to uncontrolled cell-growth impaired differentiation. We hypothesized that gene silencing mediated through aberrant promoter methylation upstream antagonist genes might result in β-catenin accumulation, resulting constitutive activation. (SFRP1, WIF1, APC CDH1) CTNNB1 was examined genomic DNA extracted from 12 urological cancer cell lines correlated with mRNA expression. Promoter status then...

MGMT downregulation in high-grade gliomas (HGG) has been mostly attributed to aberrant promoter methylation and is associated with increased sensitivity alkylating agent-based chemotherapy. However, HGG harboring 10q deletions also benefit from treatment agents. Because the gene mapped at 10q26, we hypothesized that both epigenetic genetic alterations might affect its expression predict response To test this hypothesis, mRNA levels of were determined by quantitative methylation-specific PCR...

Epigenetic alterations are common in prostate cancer (PCa) and seem to contribute decisively its initiation progression. Moreover, aberrant promoter methylation is a promising biomarker for non-invasive screening. Herein, we sought characterize EFEMP1 as PCa, unveiling biological relevance carcinogenesis. Microarray analyses of treated PCa cell lines primary tissues enabled the selection differentially methylated genes, among which was further validated by MSP bisulfite sequencing....

Urothelial carcinoma (UC) is the most common cancer affecting urinary system, worldwide. Lack of accurate early detection tools entails delayed diagnosis, precluding more efficient and timely treatment. In a previous study, we found that miR-129-2 miR-663a were differentially methylated in UC compared with other genitourinary tract malignancies. Here, evaluated diagnostic performance those microRNAs urine.Promoter methylation levels assessed, using real-time quantitative methylation-specific...

The three most common genitourinary malignancies (prostate/kidney/bladder cancers) constitute a substantial proportion of all cancer cases, mainly in the elderly population. Early detection is key to maximizing patients’ survival, but lack highly accurate biomarkers that might be used through non-/minimally invasive methods has impaired progress this domain. Herein, we sought develop minimally test detect and discriminate among those urological cancers based on miRNAs assessment ddPCR....