A5026140052- Epigenetics and DNA Methylation
- Physical Education and Sports Studies
- Adventure Sports and Sensation Seeking
- Prostate Cancer Treatment and Research
- Human Mobility and Location-Based Analysis
- Bladder and Urothelial Cancer Treatments
- Cancer-related gene regulation
- Transportation Planning and Optimization
- Physical Education and Gymnastics
- GDF15 and Related Biomarkers
- Outdoor and Experiential Education
- Sport and Mega-Event Impacts
- Urological Disorders and Treatments
- Recreation, Leisure, Wilderness Management
- Cultural, Media, and Literary Studies
- Transportation and Mobility Innovations
- Renal and related cancers
- Education and Digital Technologies
- Traffic Prediction and Management Techniques
- Birth, Development, and Health
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Physical Education and Pedagogy
- Prostate Cancer Diagnosis and Treatment
- Urban Transport and Accessibility
Université Côte d'Azur
2020-2021
Inserm
2020-2021
Centre Méditerranéen de Médecine Moléculaire
2021
Universidade do Porto
2007-2020
INESC TEC
2019-2020
University Gama Filho
2009-2017
Universidade Castelo Branco
2009-2017
University of Oslo
2010-2014
Instituto Português de Oncologia Francisco Gentil
2006-2014
Oslo University Hospital
2010-2014
TMPRSS2-ETS gene fusions have been found recurrently in prostate carcinomas, but not the presumed precursor lesion, high-grade prostatic intraepithelial neoplasia (HGPIN). However, HGPIN lesions may share chromosomal changes with cancer. To determine relative order of genetic events carcinogenesis, we analyzed 34 19 paired lesions, 14 benign hyperplasias, 11 morphologically normal tissues for TMPRSS2-ERG, TMPRSS2-ETV1 rearrangements, genomic imbalances. TMPRSS2 exon 1 was fused in-frame ERG...
To identify a panel of epigenetic biomarkers for accurate bladder cancer (BlCa) detection in urine sediments.Gene expression microarray analysis BlCa cell lines treated with 5-aza-2'-deoxycytidine and trichostatin A as well 26 tissue samples was used to list novel methylation candidates BlCa. Methylation levels candidate genes were quantified 4 lines, 50 tissues, 20 normal mucosas (NBM), sediments from 51 patients healthy donors, 19 renal patients, prostate patients. Receiver operator...
Abstract Purpose: Prostate cancer is a highly prevalent malignancy and constitutes major cause of cancer-related morbidity mortality. Owing to the limitations current clinical, serologic, pathologic parameters in predicting disease progression, we sought investigate prognostic value promoter methylation small panel genes by quantitative methylation-specific PCR (QMSP) prostate biopsies. Experimental Design: Promoter levels APC, CCND2, GSTP1, RARB2, RASSF1A were determined QMSP prospective...
Abstract Background Multiple epigenetic and genetic changes have been reported in colorectal tumors, but few of these clinical impact. This study aims to pinpoint markers that can discriminate between non-malignant malignant tissue from the large bowel, i.e. with diagnostic potential. The methylation status eleven genes ( ADAMTS1 , CDKN2A CRABP1 HOXA9 MAL MGMT MLH1 NR3C1 PTEN RUNX3 SCGB3A1 ) was determined 154 samples including normal mucosa, adenomas, carcinomas colorectum. gene-specific...
The three main types of urological cancers are mostly curable by surgical resection, if early detected. We aimed to identify novel DNA methylation biomarkers common these cancers, potentially suitable for non-invasive testing. From a candidate list markers created after gene expression assessment pharmacologically treated cell lines and tissue samples, two genes were selected further validation. Methylation levels quantified in total 12 cancer 318 clinical samples. PCDH17 TCF21 provided...
Abstract Background Multidrug resistance 1 ( MDR1 ) gene encodes for an ATP binding cassette transporter - P-glycoprotein (P-gp) involved in chemoresistance to taxanes. promoter methylation is frequent prostate carcinoma (PCa), suggesting epigenetic regulation but no functional correlation has been established. We aimed elucidate the mechanisms deregulation PCa. Results and P-gp expression were assessed 121 PCa, 39 high-grade prostatic intraepithelial neoplasia (HGPIN), 28 benign hyperplasia...
The kidney is a target organ for the toxicity of several xenobiotics and also highly susceptible to development malignant tumors. In both cases, in vitro studies provide insight cellular damage, represent adequate models study either mechanisms underlying toxic effects nephrotoxicants or therapeutic approaches renal cancer. efficient methods establishment human normal tumor cell hence crucial. this study, technically simple rapid protocol isolation culture proximal tubular epithelial cells...
Obesity is a growing societal scourge. Recent studies have uncovered that paternal excessive weight induced by an unbalanced diet affects the metabolic health of offspring. These reports mainly employed single-generation male exposure. However, consequences multigenerational feeding on progeny remain largely unknown. Here, we show maintaining Western for five consecutive generations in mice induces enhancement fat mass and related diseases over generations. Strikingly, chow-diet-fed...
A disseminação de fake news no contexto da saúde coletiva tem se consolidado como um dos desafios mais prementes atualidade, comprometendo a adesão população às recomendações científicas e impactando negativamente formulação implementação políticas públicas sanitárias. O avanço das tecnologias digitais popularização redes sociais intensificaram propagação conteúdos enganosos, favorecendo amplificação informações inverídicas que afetam desde percepção pública sobre tratamentos vacinas até...
Abstract Background Aberrant promoter hypermethylation of cancer-associated genes occurs frequently during carcinogenesis and may serve as a cancer biomarker. In this study we aimed at defining quantitative gene methylation panel that might identify the most prevalent types renal cell tumors. Methods A 18 promoters was assessed by methylation-specific PCR (QMSP) in 85 primarily resected tumors representing four major histologic subtypes (52 clear (ccRCC), 13 papillary (pRCC), 10 chromophobe...
Abstract Purpose: Zinc is involved in several physiologic processes, including cell growth and proliferation. Although normal prostate tissue zinc levels are high, there a marked decrease cancer. Metallothioneins control the bioavailability of one isoform, MT1G, was reported down-regulated Here, we investigated whether promoter methylation might cause MT1G silencing Patients Methods: The assessed by quantitative methylation-specific PCR on prospectively collected samples from 121 patients...
A large percentage of prostate cancers harbor TMPRSS2-ERG gene fusions, leading to aberrant overexpression the transcription factor ERG. The target genes deregulated by this rearrangement, however, remain mostly unknown. To address subject we performed genome-wide mRNA expression analysis on 6 non-malignant samples and 24 carcinomas with (n = 16) without 8) fusion as determined FISH. top-most differentially expressed their associations ERG over-expression were technically validated...
Nowadays, mobile phones are ubiquitous systems of our society. Nevertheless, the adoption this technology to perform payments, namely in public transport, was only implemented a few number transport networks. Thus, paper aims understand which main factors that may influence payments transport. For purpose, survey applied different groups population. The study conducted on networks medium-sized metropolitan area (Oporto-Portugal) and big-sized (Beijing-China). evaluation results current...
The proliferation of new technologies is revolutionizing the public transport sector, allowing Operators to replace complex and expensive infrastructures by travellers' mobile devices online management channels platforms. This paper aims present Seamless Mobility platform, a disruptive solution based on these channels, which main goal facilitate promote usage for travellers, as well reducing operational costs companies. platform integrates three components: (i) payments, (ii) route planner,...
Abstract The association of a genetic analysis that could improve the diagnostic accuracy renal cell tumors in biopsy samples would allow better‐informed therapeutic decisions. We performed comparative genomic hybridization (CGH) on an ex vivo fine‐needle aspiration (FNA) and tumor fragment obtained from 75 patients consecutively diagnosed with subjected to radical nephrectomy. pattern changes by CGH was used blindly classify findings were subsequently compared histopathologic diagnosis. In...
Constitutive activation of the Wnt signaling pathway is a common feature solid tumors and contributes to uncontrolled cell-growth impaired differentiation. We hypothesized that gene silencing mediated through aberrant promoter methylation upstream antagonist genes might result in β-catenin accumulation, resulting constitutive activation. (SFRP1, WIF1, APC CDH1) CTNNB1 was examined genomic DNA extracted from 12 urological cancer cell lines correlated with mRNA expression. Promoter status then...
Abstract Background Only a few studies have addressed the molecular pathways specifically involved in carcinogenesis of distal colon and rectum. We aimed to identify potential differences among genetic alterations rectal carcinomas as compared cancers arising elsewhere large bowel. Methods Constitutional tumor DNA from test series 37 patients with 25 sigmoid carcinomas, previously analyzed for microsatellite instability (MSI), was studied BAX , IGF2R TGFBR2 MSH3 MSH6 sequence alterations,...
Epigenetic alterations are common in prostate cancer (PCa) and seem to contribute decisively its initiation progression. Moreover, aberrant promoter methylation is a promising biomarker for non-invasive screening. Herein, we sought characterize EFEMP1 as PCa, unveiling biological relevance carcinogenesis. Microarray analyses of treated PCa cell lines primary tissues enabled the selection differentially methylated genes, among which was further validated by MSP bisulfite sequencing....