A5023656622- Aquaculture Nutrition and Growth
- Meat and Animal Product Quality
- Animal Nutrition and Physiology
- Algal biology and biofuel production
- Protein Hydrolysis and Bioactive Peptides
- Seaweed-derived Bioactive Compounds
- Enzyme Catalysis and Immobilization
- Adipose Tissue and Metabolism
- Enzyme Production and Characterization
- Environmental Toxicology and Ecotoxicology
- Air Quality and Health Impacts
- Physiological and biochemical adaptations
- Peroxisome Proliferator-Activated Receptors
- Rabbits: Nutrition, Reproduction, Health
- Polyamine Metabolism and Applications
- Probiotics and Fermented Foods
- Hydrogels: synthesis, properties, applications
- Marine and coastal plant biology
- Atmospheric chemistry and aerosols
- Electrolyte and hormonal disorders
- Food Chemistry and Fat Analysis
- Echinoderm biology and ecology
- Polysaccharides Composition and Applications
- Food and Agricultural Sciences
- Polysaccharides and Plant Cell Walls
University of Lisbon
2017-2024
Centre for Research in Anthropology
2017-2023
Instituto Nacional de Investigação Agrária e Veterinária
2022-2023
Ipca Laboratories (India)
2023
University of Aveiro
2012-2015
University of the Basque Country
2013
Universidade Norte do Paraná
2013
University of Algarve
2001
Herein, we investigated the effect of Chlorella vulgaris as ingredient (10% incorporation) in broiler diets, supplemented or not with 2 formulations Carbohydrate-Active enZymes (CAZymes; Rovabio Excel AP and a mixture recombinant CAZymes, composed by an exo-β-glucosaminidase, alginate lyase, peptidoglycan N-acetylmuramic acid deacetylase lysozyme), on growth performance, meat quality, fatty composition, oxidative stability, sensory traits. One hundred twenty 1-day-old Ross 308 male birds...
Abstract In this study, a rational combination of 200 pre-selected Carbohydrate-Active enzymes (CAZymes) and sulfatases were tested, individually or combined, according to their ability degrade Chlorella vulgaris cell wall access its valuable nutritional compounds. The disruption microalgae walls by four-enzyme mixture (Mix) in comparison with the control, enabled release up 1.21 g/L reducing sugars (p < 0.001), led an eight-fold increase oligosaccharides reduced fluorescence intensity...
Abstract The main goal of this study was to test a rational combination pre‐selected carbohydrate‐active enzymes (CAZymes) and sulphatases, individually or in combination, order evaluate its capacity disrupt Arthrospira platensis cell wall, allowing the release valuable nutritional bioactive compounds. By end, two‐enzyme constituted mixture (Mix), composed by lysozyme α‐amylase, incubated with A. suspension. microalga wall disruption evaluated through amount reducing sugars released from...
Green macroalgae, e.g., Ulva lactuca, are valuable bioactive sources of nutrients; but algae recalcitrant cell walls, composed a complex cross-linked matrix polysaccharides, can compromise their utilization as feedstuffs for monogastric animals. This study aimed to evaluate the ability pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases degrade U. lactuca walls release nutritive compounds. A databank 199 recombinant CAZymes was tested in vitro action towards wall...
Seaweeds, including the green Ulva lactuca, can potentially reduce competition between feed, food, and fuel. They also contribute to improved development of weaned piglets. However, their indigestible polysaccharides cell wall pose a challenge. This be addressed through carbohydrase supplementation, such as recombinant ulvan lyase. The objective our study was assess muscle metabolism piglets fed with 7% U. lactuca 0.01% lyase using an integrated transcriptomics (RNA-seq) proteomics (LC-MS)...
The effect of Spirulina (Arthrospira platensis), individually or in combination with two commercial carbohydrases, piglet diets was assessed on growth performance, nutrient digestibility and meat quality traits. Forty post-weaned male piglets from Large White × Landrace sows crossed Pietrain boars an initial live weight 12.0 ± 0.89 kg were used. Piglets assigned to one four dietary treatments (n = 10): cereal soya bean meal base diet (control), 10% (SP), SP supplemented 0.005% Rovabio® Excel...
Chlorella vulgaris (CH) is usually considered a feed supplement in pig nutrition, and its use as an ingredient poorly studied. Among many interesting characteristics, this microalga has high protein levels can be putative alternative for soybean meal. Our aim was to study the effect of 5% CH incorporation diet, individually or combined with two carbohydrases, on meat quality traits nutritional value. Forty-four post-weaned male piglets housed, initial live weight 11.2 ± 0.46 kg, were...
The purpose of this study was to evaluate the impact Chlorella vulgaris (5% in diet), supplemented or not with two exogenous carbohydrase mixtures on piglets' performance, nutrient digestibility and gut morphology, fermentation products microbiota. Forty-four male piglets weaned at 28 days age, 11.2 ± 0.46 kg live weight, were used assigned 1 4 dietary treatments: cereal soybean meal based-diet (control, n = 11), control diet 5% C. (CH, 10), CH 0.005% Rovabio® Excel AP (CH + R, 10) 0.01% a...
The influence of a high inclusion level Chlorella vulgaris, individually and supplemented with two carbohydrase mixtures, in finishing pig diets was assessed on zootechnical performance, carcass characteristics, pork quality traits nutritional value fat. Forty crossbred entire male pigs, sons Large White × Landrace sows crossed Pietrain boars, an initial live weight 59.1 ± 5.69 kg were used this trial. Swines randomly assigned to one four dietary treatments (n = 10): cereal soybean...
Laminaria digitata is a novel feedstuff that can be used in pig diets to replace conventional feedstuffs. However, its resilient cell wall prevent the monogastric digestive system from accessing intracellular nutrients. Carbohydrate-active enzyme (CAZyme) supplementation putative solution for this problem, degrading during digestion. The objective of work was evaluate effect 10% L. feed inclusion and CAZyme on meat quality nutritional value weaned piglets. Forty piglets were randomly...
Abstract In the present study, 199 pre-selected Carbohydrate-Active enZymes (CAZymes) and sulfatases were assessed, either alone or in combination, to evaluate their capacity disrupt Laminaria digitata cell wall, with consequent release of interesting nutritional compounds. A previously characterized individual alginate lyase, belonging family 7 polysaccharide lyases (PL7) produced by Saccharophagus degradans , was shown be most efficient vitro degradation L. wall. The lyase treatment,...
We hypothesized that dietary inclusion of 15% Laminaria digitata, supplemented or not with carbohydrases, could improve the nutritional value poultry meat without impairing animal growth performance. A total 120 22-day old broilers were fed following treatments (n = 10) for 14 days: cereal-based diet (control); control L. digitata (LA); LA 0.005% Rovabio® Excel AP (LAR); 0.01% alginate lyase (LAE). Final body weight was lower and feed conversion ratio higher than control. The ileal viscosity...
Responses of leaf growth and water relations to seasonal drought were monitored during two successive years in three cultivars (Galhosa, Espargal Mulata) Ceratonia siliqua L. growing the field southern Portugal. Leaf fully expanded leaves characterized by pressure–volume analysis, morphometric measurements petiolar xylem made. The differed with respect onset initiation. In Galhosa, there was a sharp peak initiation June that immediately followed period when expansion rates highest. occurred...
The influence of a high inclusion level Chlorella vulgaris, individually and supplemented with two carbohydrase mixtures, in finishing pig diets was assessed on zootechnical performance, carcass characteristics, pork quality traits nutritional value fat. Forty crossbred entire male pigs, sons Large White &times; Landrace sows crossed Pietrain boars, an initial live weight 59.1 &plusmn; 5.69 kg were used this trial. Swines randomly assigned to one four dietary treatments (n=10):...
The implication of high dietary level Chlorella vulgaris, individually and supplemented with two carbohydrase mixtures, on pigs' health liver metabolism was assessed in this study. Forty crossbred (Large White × Landrace sows crossed Pietrain boars) entire male pigs were randomly allocated to the following feeding treatments (n = 10): cereal-soybean meal basal diet (control); 5% C. vulgaris; vulgaris 0.005% Rovabio® Excel AP; 0.01% a preselected four-CAZyme mixture. trial lasted from 59.1 ±...
The present study aims to assess the effects of pig's genotype (lean v. fatty) and dietary protein level (control reduced) on intramuscular fat (IMF) content, fatty acid composition fibre profile psoas major, a representative red muscle in carcass scarcely studied relative white longissimus lumborum. experiment was conducted 40 intact male pigs (20 Alentejana purebred 20 Large White×Landrace×Pietrain crossbred) from 60 93 kg live weight. Pigs were divided allocated four groups: control diet...
Abstract Background Chlorella vulgaris has been proposed as a sustainable green feedstock in poultry nutrition due to its ease of cultivation, minimal environmental impact and balanced nutritional composition. However, the majority studies documents use C. dietary supplement broilers instead feed ingredient. To best our knowledge, no report shown effect high-level incorporation (>2 % diet) on plasma metabolites hepatic lipid composition broilers. One hundred twenty Ross 308 male birds...
Monogastric feeding is dependent on costly conventional feedstuffs. Microalgae such as Chlorella vulgaris are a sustainable alternative; however, its recalcitrant cell wall hinders monogastric digestion. Carbohydrate Active Enzyme (CAZyme) supplementation possible solution. The objective of this work was to evaluate the effect 5% dietary C. (CV) and enzymatic (CV + R-Rovabio® Excel AP; CV M-four CAZyme mix) muscle transcriptome proteome finishing pigs, in an integrated approach. Control pigs...