A5032383389- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Metabolomics and Mass Spectrometry Studies
- Fatty Acid Research and Health
- Analytical Chemistry and Chromatography
- Heavy Metals in Plants
- Cancer, Lipids, and Metabolism
- Phytochemicals and Antioxidant Activities
- Tea Polyphenols and Effects
- Natural product bioactivities and synthesis
- Phytochemistry Medicinal Plant Applications
- Antioxidant Activity and Oxidative Stress
- Adipokines, Inflammation, and Metabolic Diseases
- Aquaculture Nutrition and Growth
- Pharmacogenetics and Drug Metabolism
- Drug Solubulity and Delivery Systems
- Bee Products Chemical Analysis
- Enzyme Production and Characterization
- Electrochemical sensors and biosensors
- Adipose Tissue and Metabolism
- Essential Oils and Antimicrobial Activity
- Lipid metabolism and biosynthesis
- Lipid Membrane Structure and Behavior
- Therapeutic Uses of Natural Elements
- Natural Antidiabetic Agents Studies
Universidade São Francisco
2016-2025
Hospital São Lucas da PUCRS
2023
University of Lisbon
2022
University of Coimbra
2013-2021
Universidade Federal de São Paulo
2016
Universidade de São Paulo
2008-2016
Universidade Estadual de Campinas (UNICAMP)
1998-2016
Creative Research Enterprises (United States)
2016
Grupo Santa Casa de Belo Horizonte
2014
Cambridge University Press
2009
Aqueous extracts of green yerba maté (Ilex paraguariensis) and tea (Camellia sinensis) are good sources phenolic antioxidants, as already described in the literature. The subject this study were organic from maté, both roasted, tea. Their profiles characterized by direct infusion electrospray insertion mass spectrometry (ESI-MS) their free radical scavenging activity was determined DPPH assay. Organic containing antioxidants might be used natural food industry, replacing synthetic additives...
During the extraction of propolis several factors were varied in order to determine how they affected yield and phenolic composition obtained extracts. Six samples green from Southeastern region Brazil tested. The results indicate that highest extracts by maceration, which also had greatest number components, those using 70% (v/v) ethanol or more as a solvent. Soxhlet procedure resulted even higher yields. No differences observed between macerated presence absence light. An increase was for...
Because the potential of yerba maté (Ilex paraguariensis) has been suggested in management obesity, aim present study was to evaluate effects extract on weight loss, obesity-related biochemical parameters, and regulation adipose tissue gene expression high-fat diet-induced obesity mice. Thirty animals were randomly assigned three groups. The mice introduced standard or diets. After 12 weeks a diet, according treatment (water 1.0 g/kg). intervention, plasma concentrations total cholesterol,...
Yerba mate (Ilex paraguariensis) is rich in several bioactive compounds that can act as free radical scavengers. Since oxidative DNA damage involved various pathological states such cancer, the aim of this study was to evaluate antioxidant activity tea well ability influence repair male Swiss mice. Forty animals were randomly assigned four groups. The received three different doses aqueous extract, 0.5, 1.0 or 2.0 g/kg, for 60 days. After intervention, liver, kidney and bladder cells...
Bioavailability and biological properties of flavonoid glycosides can be improved after the enzymatic hydrolysis specific glycosyl groups. In this study, we evaluate antioxidant antiproliferative potential rutin performed by α-l-rhamnosidases (hesperidinase from Penicillium sp. naringinase decumbens) previously heated at 70°C for 30 min to inactivate undesirable β-d-glucosidase activity. The highest in vitro activity determined DPPH radical scavenging was achieved with hydrolyzed...
Lipases are one of the most used enzymes in pharmaceutical industry due to their efficiency organic syntheses, mainly production enantiopure drugs. From an industrial viewpoint, selection efficient expression system and host for recombinant lipase is highly important. The hosts Escherichia coli Komagataella phaffii (previously known as Pichia pastoris) less often reported Bacillus Aspergillus strains. use systems overproduce homologous or heterologous lipases require strong promoters...
The aim of the present study was to evaluate effects yerba maté extract upon markers insulin resistance and inflammatory in mice with high fat diet-induced obesity. were introduced either standard or diets. After 12 weeks on a diet, randomly assigned one two treatment conditions, water at 1.0 g kg−1. treatment, glucose blood level hepatic soleus muscle response evaluated. Serum levels TNF-α IL-6 evaluated by ELISA, liver tissue examined determine mRNA TNF-α, iNOS, nuclear translocation NF-κB...
The inhibitory effects of maté tea (MT), a beverage produced with leaves from Ilex paraguariensis, in vitro lipase activity and on obesity obese mice models were examined. For the experiment, porcine human pancreatic (PL) activities determined by measuring rate release oleic acid hydrolysis olive oil emulsified taurocholate, phospholipids, gum arabic, or polyvinyl alcohol. vivo experiments, animals fed standard diet (SD, n = 10) high-fat (HFD, 30) for 16 weeks. After first 8 weeks HFD,...
The aim of this study was to evaluate the effects green tea Camellia sinensis extract on proinflammatory molecules and lipolytic protein levels in adipose tissue diet-induced obese mice. Animals were randomized into four groups: CW (chow diet water); CG water + extract); HW (high-fat HG extract). mice fed ad libitum with chow or high-fat concomitantly supplemented (oral gavage) 400 mg/kg body weight/day (CG HG, resp.). treatments performed for eight weeks. UPLC showed that 10 mg/mL extract,...
The antioxidant activity of maté tea, the roasted product derived from yerba (Ilex paraguarienis), was observed in vitro and animal models, but studies humans are lacking. aim this study to investigate effects tea supplementation on plasma susceptibility oxidation enzyme gene expression healthy nonsmoking women, after acute or prolonged ingestion. We evaluated total status (TAS), kinetics diene conjugate generation, thiobarbituric acid reactive substance (TBARS) contents plasma, as well mRNA...
Abstract Lipid content and fatty acid composition were determined in edible meat of fifteen marine fish species caught on the Southeast Brazilian coast two from East Antarctic. Most had lipid amounts lower than 10% their total weight. Palmitic (C16:0) predominated, accounting for 54–63% amount saturated acids. Oleic (C18:1n‐9) was most abundant (49–69%) monounsaturated acid, docosahexaenoic (DHA) predominant polyunsaturated (PUFA), 31–84% n‐3 PUFA. PUFA level highest Antarctic meat,...
Progenesis QI (PQI) is a multiplatform bioinformatics tool that facilitates the identification workflow for metabolomics experiments. PQI uses fragmentation data provided by MassBank of North America (MoNA) libraries, among others, metabolite annotation. However, does not officially support MoNA libraries and other based on structure-data files (.sdf). This paper describes development application software named to Library Converter, allowing correcting library readability. We evaluated...
Background/Objectives: This study investigates the metabolic profile of a single dose etodolac in healthy volunteers, focusing on pharmacokinetics, clinical parameters, and metabolomic variations to identify biomarkers pathways linked drug response, efficacy, safety. Methods: Thirty-seven enrolled after rigorous health assessments, received (Flancox® 500 mg). Pharmacokinetic profiles were determined using tandem mass spectrometry analysis, profiling was conducted baseline samples (pre-dose)...
Yerba maté (Ilex paraguariensis) is rich in polyphenols, especially chlorogenic acids. Evidence suggests that dietary polyphenols could play a role glucose absorption and metabolism. The aim of this study was to evaluate the antidiabetic properties yerba extract alloxan-induced diabetic Wistar rats. Animals (n = 41) were divided four groups: nondiabetic control (NDC, n 10), (NDY, (DC, 11), (DY, 10). intervention consisted administration 1 g extract/kg body weight dose for 28 days; controls...
The flavonoids are mainly present in Citrus fruits as their glycosyl derivatives. This study was conducted comparing vitro xanthine oxidase inhibitory activity of the aglycone hesperetin and its glycosylated forms (hesperidin G-hesperidin) effects on plasma lipid profile oxidative-antioxidative system (TBARS antioxidant enzymes) rats. concentrations major conjugated metabolites rat after oral administration these compounds were also determined. Wistar male rats randomly assigned to three...
POTENTIAL OF ENANTIOSELECTIVE BIOCATALYSIS BY MICROBIAL LIPASES.Microbial lipases have a great potential for commercial applications due to their stability, selectivity and broad substrate specificity because many non-natural acids, alcohols or amines can be used as the substrate.Three microbial isolated from Brazilian soil samples (Aspergillus niger; Geotrichum candidum; Penicillium solitum) were compared in terms of stability biocatalysts enantioselective esterification using racemic...