A5086328958- Nuclear Structure and Function
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Cancer-related gene regulation
- Erythrocyte Function and Pathophysiology
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Phagocytosis and Immune Regulation
- Renal and related cancers
- Heat shock proteins research
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Wnt/β-catenin signaling in development and cancer
- Extracellular vesicles in disease
- Hippo pathway signaling and YAP/TAZ
- Neurobiology and Insect Physiology Research
- Skin and Cellular Biology Research
- Protein Structure and Dynamics
- Developmental Biology and Gene Regulation
- Prenatal Screening and Diagnostics
- Endoplasmic Reticulum Stress and Disease
Centre National de la Recherche Scientifique
2019-2025
Université Paris-Saclay
2025
CEA Paris-Saclay
2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2025
Institut de Biologie Intégrative de la Cellule
2025
Université Paris Cité
2019-2023
Institut Jacques Monod
2019-2023
Sorbonne Université
2020-2023
Génétique et biologie du développement
2021-2023
Institut Curie
2020-2023
Fiber tension enables tissue scaling Tissue development, homeostasis, and repair require cells to sense mechanical forces. Although many molecular actors implicated in cell mechanosensitivity have been extensively studied, the basis by which adapt their responses geometry remains poorly defined. López-Gay et al. now identify how two fundamental epithelial structures—stress fibers tricellular junctions—endow Drosophila with an internal ruler scale response area. This work explains of...
Cell proliferation is central to epithelial tissue development, repair, and homeostasis. During cell division, small RhoGTPases control both actomyosin dynamics cell-cell junction remodeling faithfully segregate the genome while maintaining polarity integrity. To decipher mechanisms of RhoGTPase spatiotemporal regulation during we generated a transgenic fluorescently tagged library for 48 Drosophila Rho guanine exchange factors (RhoGEFs) GTPase-activating proteins (GAPs), systematically...
Deciphering gene function requires the ability to control expression in space and time. Binary systems such as Gal4/UAS provide a powerful means modulate induce loss or gain of function. This is best exemplified Drosophila, where system has been critical discover conserved mechanisms development, physiology, neurobiology, metabolism, cite few. Here we describe transgenic light-inducible (ShineGal4/UAS) based on Magnet photoswitches. We show that it allows efficient, rapid, robust activation...
Abstract Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic post-transcriptional levels. However, extent their regulatory potential underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107 , hosted in fission yeast long mamRNA carrying two independent cellular functions. On one hand, guides site-specific...
The Unfolded Protein Response (UPR) is a cellular pathway activated by sensory proteins, including the protein kinase PERK (EIF2AK3), that monitors perturbations in endoplasmic reticulum (ER). Using tunicamycin, which induces ER stress thwarting N-glycosylation, we monitored system-wide changes proteome using PISA (Proteome Integral Solubility Alteration) and abundance analysis. Global proteomics revealed precise membrane- ER-associated proteins through widespread induction of degradation...
Besides assembling nuclear pore complexes, the conduits of transport, many nucleoporins also contribute to chromatin organization and gene expression, with critical roles in development pathologies. We previously reported that Nup133 Seh1, two components Y-complex subassembly scaffold, are dispensable for mouse embryonic stem cell viability but required their survival during neuroectodermal differentiation. Here, a transcriptomic analysis revealed regulates subset genes at early stages...
Small nucleolar RNAs are non-coding transcripts that guide chemical modifications of RNA substrates and modulate gene expression at the epigenetic post-transcriptional levels. However, extent their regulatory potential underlying molecular mechanisms remain poorly understood. Here, we identify a conserved, previously unannotated intronic C/D-box snoRNA, termed snR107, hosted in fission yeast long mamRNA carrying two independent cellular functions. On one hand, snR107 guides site-specific 25S...
Abstract Although structural nuclear pore proteins (nucleoporins) are seemingly required in every cell type to assemble a functional transport machinery, mutations or deregulation of subset them have been associated with specific human hereditary diseases. In particular, previous genetic studies patients nephrotic syndrome identified Nup107 that impaired the expression localization its direct partner at pores, Nup133. present study, we characterized zebrafish nup133 orthologous gene and...
Summary Planar cell polarity (PCP) organizes the orientation of cellular protrusions and migratory activity within tissue plane. PCP establishment involves subcellular polarization core components. It has been suggested Wnt gradients could provide a global cue that coordinates local with axes. Here we dissect role ligands in hairs Drosophila wings, an established system for study PCP. We found was normal quintuple mutant wings rely solely on membrane-tethered Wingless signaling, suggesting...
Summary Cell proliferation is central to epithelial tissue development, repair and homeostasis. During cell division, small RhoGTPases control both actomyosin dynamics cell-cell junction remodelling faithfully segregate the duplicated genome while maintaining polarity integrity. To decipher mechanisms of spatiotemporal regulation during we generated a transgenic fluorescently tagged library for Drosophila Rho Guanine exchange factors (GEF) GTPase activating proteins (GAP), systematically...
ABSTRACT From their essential function in building up the nuclear pore complexes, nucleoporins have expanded roles beyond transport. Hence, contribution to chromatin organization and gene expression has set them as critical players development pathologies. We previously reported that Nup133 Seh1, two components of Y-complex subunit scaffold, are dispensable for mouse embryonic stem cell viability but required survival during neuroectodermal differentiation. Here, a transcriptomic analysis...
An amendment to this paper has been published and can be accessed via a link at the top of paper.