A5101925688- Cellular Mechanics and Interactions
- Neuroscience and Neuropharmacology Research
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Pickering emulsions and particle stabilization
- Genomics and Chromatin Dynamics
- Nuclear Structure and Function
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Hippo pathway signaling and YAP/TAZ
- Force Microscopy Techniques and Applications
- Retinal Development and Disorders
- Developmental Biology and Gene Regulation
- bioluminescence and chemiluminescence research
- Neural dynamics and brain function
- Analytical Chemistry and Sensors
- Ion Channels and Receptors
- Proteins in Food Systems
- Advanced biosensing and bioanalysis techniques
- Heat shock proteins research
- Memory and Neural Mechanisms
- Neuroscience and Neural Engineering
- Connexins and lens biology
- Cell Adhesion Molecules Research
- Material Dynamics and Properties
École Polytechnique
2021-2023
Centre National de la Recherche Scientifique
2011-2023
Laboratoire d'Hydrodynamique
2021-2023
Institut Curie
2016-2020
Université Paris Sciences et Lettres
2020
Inserm
2008-2020
Université Paris Cité
2017-2020
Sorbonne Université
2017-2020
Aix-Marseille Université
2012-2019
Génétique et biologie du développement
2016-2017
Fiber tension enables tissue scaling Tissue development, homeostasis, and repair require cells to sense mechanical forces. Although many molecular actors implicated in cell mechanosensitivity have been extensively studied, the basis by which adapt their responses geometry remains poorly defined. López-Gay et al. now identify how two fundamental epithelial structures—stress fibers tricellular junctions—endow Drosophila with an internal ruler scale response area. This work explains of...
Nuclear shape is different in stem cells and differentiated reflects important changes the mechanics of nuclear envelope (NE). The current framework emphasizes key role lamina its alterations disease. Whether active stress controls deformations how this interplays with properties NE to control dynamics unclear. We address early Drosophila embryo, which profound parallel transcriptional activation zygotic genome. show that microtubule (MT) polymerization events produce elementary forces...
Hippocalcin is a Ca(2+)-binding protein, which belongs to the family of neuronal Ca(2+) sensors. It highly expressed in hippocampus but molecular mechanisms underlying its action this part brain have not been investigated detail. To study whether intrinsic activity could result hippocalcin-mediated signal transduction we examined spontaneous and potential (AP)-dependent changes fluorescence yellow fluorescent protein-tagged hippocalcin (HPCA-YFP) transiently transfected hippocampal cultured...
Abstract Calcium ion acts in nearly every aspect of cellular life. The versatility and specificity required for such a ubiquitous role is ensured by the spatio-temporal dynamics calcium concentration variations. While signal has been extensively studied cell cultures adult tissues, little known about activity during early tissue morphogenesis. We monitored intracellular Drosophila gastrula revealed single spikes that were short-lived, rare showed strong variability among embryos....
Genetically encoded probes have become powerful tools for non-invasive monitoring of ions, distributions proteins and the migration formation cellular components. We describe functional expression two molecular fluorescent intracellular Cl ([Cl] i ) functioning glycine receptor (GlyR) channels. The first probe is a recently developed cyan protein–yellow protein-based construct, termed Cl-Sensor, with relatively high sensitivity to ( K app ∼30 mM). In this study, we its in retina cells using...
We have previously observed that in response to antigenic activation, T cells produce actin-rich protrusions generate forces involved cell activation. These are influenced by the mechanical properties of antigen-presenting (APCs). However, how external forces, which can be produced APCs, influence dynamic actin protrusion remains unknown. In this study, we quantitatively characterised effects grown activated cells.Using a micropipette force probe, applied controlled compressive or pulling on...
Systems of complex particles such as proteins or colloidal have a widely observed tendency to form nonconnected nanometer-size clusters at steady state, but the underlying mechanisms remain poorly understood. We report here numerical study on self-aggregation low-valence with flexible bonds (i.e., free bond orientations) in two dimensions and predict formation stable cluster phase for average valences ranging from 2 3.6. For intermediate case trivalent particles, we show that is present over...
We compute the structure and kinetics of two systems low-valence particles with three or six freely oriented bonds in dimensions. The clusters formed by trivalent is complex loops holes, while hexavalent self-organize into regular compact structures. identify elementary structures which compose particles. At initial stages clustering, grow a power-law time dependence. Yet at longer times fusion fission equilibrates form heterogeneous phase polydispersed sizes. These results emphasize role...
Nuclear shape is different in stem cells and differentiated reflects important changes the mechanics of nuclear envelope (NE). The current framework emphasizes key role lamina its alterations disease. Whether active stress controls deformations how this interplays with properties NE to control dynamics unclear. We address early Drosophila embryo, which profound parallel transcriptional activation zygotic genome. show that microtubule (MT) polymerization events produce elementary forces...
Calcium signaling plays a crucial role in the physiology of organs but also various aspects organogenesis embryo. High versatility calcium is encoded by dynamic variation intracellular concentration. While dynamics important, little known about it throughout embryogenesis largest class animals, insects. Here, we visualize Drosophila using fluorescent protein-based indicator, GCaMP3, and report transients epithelium neuronal tissues. Local varying duration were detected outer epithelium,...