A5034755889- Phytase and its Applications
- Plant tissue culture and regeneration
- Wheat and Barley Genetics and Pathology
- Plant Micronutrient Interactions and Effects
- Enzyme-mediated dye degradation
- Plant-Microbe Interactions and Immunity
- Enzyme Production and Characterization
- Genetics and Plant Breeding
- Genetic Mapping and Diversity in Plants and Animals
- Pancreatic function and diabetes
- Food composition and properties
- Plant Genetic and Mutation Studies
- Insect Resistance and Genetics
- Diabetes and associated disorders
- Metabolism, Diabetes, and Cancer
- Plant Gene Expression Analysis
- Biofuel production and bioconversion
- Protein Hydrolysis and Bioactive Peptides
- Plant Reproductive Biology
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Plant Stress Responses and Tolerance
- Folate and B Vitamins Research
- Crop Yield and Soil Fertility
- Photosynthetic Processes and Mechanisms
Roskilde Sygehus
2024
University of Copenhagen
2014-2023
Rigshospitalet
2021
Symphogen (Denmark)
2006-2019
Danish National Institute of Public Health
2008
Technical University of Denmark
1996-2007
Steno Diabetes Centers
1997-2004
Nordic Laboratory for Luminescence Dating
1990-2004
Centre for Sustainable Energy
2004
Aalborg Portland (Denmark)
1999-2003
Understanding peroxidase function in plants is complicated by the lack of substrate specificity, high number genes, their diversity structure and our limited knowledge gene transcription translation. In present study we sequenced expressed sequence tags (ESTs) encoding novel heme‐containing class III peroxidases from Arabidopsis thaliana annotated 73 full‐length genes identified genome. total, transcripts 58 these have now been observed. The expression individual was assessed organ‐specific...
The satellite sequence studied was primarily composed of GAA repeats organized in long tracts heterochromatic DNA. Fluorescent situ hybridization (FISH) with the (GAA banding) to chromosomes barley, wheat, rye, and other Triticeae species produced banding patterns similar those obtained by N-banding. GAA-banding barley are described detail 12 briefly. In GAA-satellite permits identification all barley. It is a valuable alternative techniques, especially connection physical gene mapping FISH....
The clinical use of dendritic cells (DCs) to induce antigen-specific immune tolerance has been hampered by the lack a widely acknowledged method for generating human regulatory DCs but even more so non-existence reliable markers. Thus, we set out find markers that can be measured with simple methods identify are applicable future studies. Human were generated from peripheral blood monocytes in presence 1alpha,25-dihydroxyvitamin D(3) (VD3), which gave rise phenotype resembles immature DCs,...
Two hundred sixteen barley ( Hordeum vulgare L.) cultivars were selected to represent the diversity and history of European spring two‐row breeding search for alleles controlling agronomic traits by association genetics. The germplasm was genotyped with 7864 gene‐based single nucleotide polymorphism markers corresponding field trial trait data relating growth straw strength obtained at multiple sites. Analysis marker statistical population genetics approaches revealed two important trends in...
Abstract A cDNA clone encoding the Prx7 peroxidase from barley (Hordeum vulgare L.) predicted a 341-amino acid protein with molecular weight of 36,515. N- and C-terminal putative signal peptides were present, suggesting vacuolar location peroxidase. Immunoblotting reverse-transcriptase polymerase chain reaction showed that mRNA accumulated abundantly in coleoptiles leaf epidermis inoculated powdery mildew fungus (Blumeria graminis). Two isoperoxidases isoelectric points 9.3 7.3 (P9.3 P7.3,...
Agmatine coumaroyltransferase (ACT), which catalyzes the first step in biosynthesis of antifungal hydroxycinnamoylagmatine derivatives, was purified to apparent homogeneity from 3-day-old etiolated barley (Hordeum vulgare L.) seedlings. The enzyme highly specific for agmatine as acyl acceptor and had highest specificity p-coumaroyl-CoA among various donors with a activity 29.7 nanokatal x mg(-1) protein. Barley ACT found be single polypeptide chain 48 kDa pI 5.20 determined by isoelectric...
Wheat phytase was purified to investigate the action of enzyme toward its pure substrate (phytic acid − myo-inositol hexakisphosphate) and naturally occurring (phytate globoids). Phytate globoids were homogeneity from wheat bran, their nutritionally relevant parameters quantified by ICP-MS. The main components phytic (40% w/w), protein (46% several minerals, in particular, K > Mg Ca Fe (in concentration order). Investigation kinetics revealed that Km Vmax decreased 29 37%, respectively, when...
Six cDNA clones encoding parts of protein Z, a major barley endosperm albumin, have been identified. Nucleotide and amino acid sequencing established 180 residues long C‐terminal sequence Z as well two minor sequences (14 7 residues). These show that is homologous with human α 1 ‐antitrypsin, otj‐antichymotrypsin, antithrombin III, mouse contrapsin chicken ovalbumin (26–32% the in identical positions). The homology specific cleavage at bond corresponding to reactive site inhibitors indicate...
We describe the expression and consistent production of a first target-specific recombinant human polyclonal antibody. An anti-Rhesus D antibody, Sym001, comprised 25 unique IgG1 antibodies, was produced by novel Sympress technology. This strategy is based on site-specific integration antibody genes in CHO cells, using FRT/Flp-In recombinase system. allows construct at same genomic site host thereby reducing position effects. Different bioreactor batches Sym001 displayed highly manufacturing...
Genome-wide association studies (GWAS) for amylose, amylopectin and β-glucan concentration in a collection of 254 European spring barley varieties allowed to identify 20, 17 21 single nucleotide polymorphic (SNP) markers, respectively, associated with these important grain quality traits. Negative correlations between the content amylose (R=-0.62, P<0.01) (R= -0.487, were found this large varieties. Besides HvCslF6, amo1 AGPL2, sex6 waxy identified among major genes responsible β-glucan,...
A collection of 100 wheat varieties representing more than years wheat-breeding history in Scandinavia was established order to identify marker-trait associations for plant height, grain yield and biomass potential bioethanol production. The field-grown material showed variations height from 54 122 cm 2 6.61 t ha-1. release monomeric sugars determined by high-throughput enzymatic treatment ligno-cellulosic varied between 0.169 0.312 g/g dm glucose 0.146 0.283 xylose. As expected, be highly...