A5071579661- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Cellular transport and secretion
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Plant pathogens and resistance mechanisms
- Machine Learning in Healthcare
- AI in cancer detection
- Photosynthetic Processes and Mechanisms
- Domain Adaptation and Few-Shot Learning
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Dementia and Cognitive Impairment Research
- Extracellular vesicles in disease
- COVID-19 diagnosis using AI
- Polysaccharides and Plant Cell Walls
- Advanced Neuroimaging Techniques and Applications
- Genomics and Phylogenetic Studies
- Plant Gene Expression Analysis
- Cancer-related molecular mechanisms research
- Brain Tumor Detection and Classification
- Artificial Intelligence in Healthcare and Education
- Plant Stress Responses and Tolerance
- Plant Pathogens and Fungal Diseases
University of Copenhagen
2012-2025
Planta
2021-2022
University of Tübingen
2012-2020
Institute of Plant Molecular Biology
2012-2014
Institut thématique Génétique, génomique et bioinformatique
2012
Carlsberg Laboratory
2006-2008
Penetration resistance to powdery mildew fungi, conferred by localized cell wall appositions (papillae), is one of the best-studied processes in plant innate immunity. The syntaxin PENETRATION (PEN)1 required for timely appearance papillae, which contain callose and extracellular membrane material, as well PEN1 itself. Appearance material papillae suggests secretion exosomes. These are potentially derived from multivesicular bodies (MVBs), supported our observation that ARA6-labeled...
Membrane trafficking is essential to fundamental processes in eukaryotic life, including cell growth and division. In plant cytokinesis, post-Golgi mediates a massive flow of vesicles that form the partitioning membrane but its regulation remains poorly understood. Here, we identify functionally redundant Arabidopsis ARF guanine-nucleotide exchange factors (ARF-GEFs) BIG1-BIG4 as regulators trafficking, mediating late secretion from trans-Golgi network not recycling endocytosed proteins...
We identified a recessive, brassinolide-insensitive mutant caused by deletion allele (bri1-201) of the brassinosteroid (BR) receptor BRI1. The bri1-201 displayed altered expression levels genes differentially regulated gibberellin (GA). RNA-blot analysis revealed that BR and GA antagonistically regulate accumulation mRNAs GA-responsive GASA1 gene, as well GA-repressible GA5 gene. Expression studies with cycloheximide indicated antagonistic effects on require de novo protein synthesis....
Abstract Host cell vesicle traffic is essential for the interplay between plants and microbes. ADP-ribosylation factor (ARF) GTPases are required budding, we studied role of these enzymes to identify important transport pathways in plant–powdery mildew interaction. A combination transient-induced gene silencing transient expression inactive forms ARF provided evidence that barley (Hordeum vulgare) ARFA1b/1c function preinvasive penetration resistance against powdery mildew, manifested by...
Accurate prediction of progression in subjects at risk Alzheimer's disease is crucial for enrolling the right clinical trials. However, a prospective comparison state-of-the-art algorithms predicting onset and currently lacking. We present findings "The Disease Prediction Of Longitudinal Evolution" (TADPOLE) Challenge, which compared performance 92 from 33 international teams future trajectory 219 individuals disease. Challenge participants were required to make prediction, each month 5-year...
Filamentous fungal and oomycete plant pathogens that invade by direct penetration through the leaf epidermal cell wall cause devastating diseases. Plant preinvasive immunity toward nonadapted filamentous is highly effective durable. Pre- postinvasive correlates with formation of evolutionarily conserved cell-autonomous structures, named papillae encasements, respectively. Yet, it still unresolved how papillae/encasements are formed whether these defense structures prevent pathogen ingress....
The foliar microbiome can extend the host plant phenotype by expanding its genomic and metabolic capabilities. Despite increasing recognition of importance for fitness, stress physiology, yield, diversity, function, contribution microbiomes to phenotypic traits remain largely elusive. recent adoption high-throughput technologies is helping unravel diversityand spatiotemporal dynamics microbiomes, but we have yet resolve their functional growth, development, ecology. Here, focus on processes...
Many filamentous plant pathogens place specialized feeding structures, called haustoria, inside living host cells. As haustoria grow, they are believed to manipulate cells generate a specialized, still enigmatic extrahaustorial membrane (EHM) around them. Here, we focused on revealing properties of the EHM. With help membrane-specific dyes and transient expression membrane-associated proteins fused fluorescent tags, studied nature EHM generated by barley leaf epidermal powdery mildew...
Plant innate immunity can effectively prevent the proliferation of filamentous pathogens. Papilla formation at site attack is essential for preinvasive immunity; in postinvasive immunity, encasement pathogen structures inside host cells hamper disease. Whereas papillae are highly dependent on transcytosis premade material, little known about formation. Here, we show that endosome-associated VPS9a, conserved guanine-nucleotide exchange factor activating Rab5 GTPases, required both pre- and...
Multi-modal models require aligned, shared embedding spaces. However, common CLIP-based approaches need large amounts of samples and do not natively support 3D or tabular data, both which are crucial in the medical domain. To address these issues, we revisit CLIP-style alignment by training a domain-specific foundation model as an image encoder demonstrate that modality is feasible with only 62 MRI scans. Our approach enabled simple accumulation strategy required for 3D, scales amount...
Summary The gibberellin (GA) 20‐oxidase encoded by Arabidopsis GA5 catalyzes the synthesis of active GAs. is a regulatory step in GA biosynthesis as mRNA levels are negatively regulated its bioactive products. A fusion between promoter and firefly luciferase reporter ( ‐ LUC ) was shown to be similarly regulated, indicating feedback occurs at transcriptional level. fidelity permitted genetic screen identify mutants altered transgene expression. This bioimaging identified two types recessive...
Encasements formed around haustoria and biotrophic hyphae as well hypersensitive reaction (HR) cell death are essential plant immune responses to filamentous pathogens. In this study we examine the components that may contribute absence of these in susceptible barley attacked by powdery mildew fungus. We find effector CSEP0162 from pathogen targets MONENSIN SENSITIVITY1 (MON1), which is important for fusion multivesicular bodies their target membranes. Overexpression silencing MON1 both...
Penetration resistance against powdery mildews is one of the best-studied processes plant innate immunity. One vital component syntaxin, PEN1, which required for timely deposition callose and extracellular membrane material, as well PEN1 itself, at attack sites. Recently, we reported that ARF-GEF GNOM also penetration resistance, mediating transport recycled including to site attack. The close relative SYP122, does not accumulate sites nor it affect resistance. In support this, show here in...
To understand the function of membrane proteins, it is imperative to know their topology. For such studies, a split green fluorescent protein (GFP) method useful. GFP barrel-shaped, consisting 11 β-sheets. When first ten β-sheets (GFP1-10) and 11th β-sheet (GFP11) are expressed from separate genes they will self-assembly reconstitute protein. However, this only occur when two domains co-localize in same cellular compartment. We have developed an easy-to-use Gateway vector set for determining...
Membrane trafficking maintains the organization of eukaryotic cell and delivers cargo proteins to their subcellular destinations, such as sites action or degradation. The formation membrane vesicles requires activation ADP-ribosylation factor ARF GTPase by SEC7 domain guanine-nucleotide exchange factors (ARF-GEFs), resulting in recruitment coat GTP-bound ARFs. In vitro assays were done with monomeric proteins, although ARF-GEFs form dimers vivo. This feature is conserved across eukaryotes,...
Disease progression modeling (DPM) using longitudinal data is a challenging task in machine learning for healthcare that can provide clinicians with better tools diagnosis and monitoring of disease. Existing DPM algorithms neglect temporal dependencies among measurements make parametric assumptions about biomarker trajectories. In addition, they do not model multiple biomarkers jointly need to align subjects' this paper, recurrent neural networks (RNNs) are utilized address these issues....