A5016190555- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Plant biochemistry and biosynthesis
- Bacterial Genetics and Biotechnology
- Enzyme Structure and Function
- Microbial Metabolites in Food Biotechnology
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Biochemical and biochemical processes
- Phytase and its Applications
- Biochemical and Structural Characterization
- Microbial Natural Products and Biosynthesis
- Photosynthetic Processes and Mechanisms
- Transgenic Plants and Applications
- Biochemical Acid Research Studies
- Genomics and Phylogenetic Studies
- Steroid Chemistry and Biochemistry
- Pharmacological Effects of Natural Compounds
- Proteoglycans and glycosaminoglycans research
Southwest University
2025
Yibin University
2025
Jiangnan University
2014-2024
Shandong Agricultural University
2010-2018
Shenyang Agricultural University
2017
Abstract Research on proteins is in rapid development, but the use of wild‐type under industrial conditions has limitations, low thermostability particular. Thermal stability includes three types: thermodynamic, kinetic, and process stability. Several factors (e.g., hydrophobic interactions hydrogen bonds) affect proteins. The rational design to improve protein thermal a hot topic field computational biology engineering. methods have been applied successfully protein, including introducing...
The synthesis of vitamin D3 precursor 7-dehydrocholesterol (7-DHC) by microbial fermentation has much attracted attention owing to its advantages environmental protection. In this study, Saccharomyces cerevisiae was engineered for a de novo biosynthesis 7-DHC. First, seven essential genes (six endogenous and one heterologous gene) were overexpressed, the ROX1 gene (heme-dependent repressor hypoxic genes) knocked out. resulting strain produced 82.6 mg/L 7-DHC from glucose. Then, we predicted...
Fructosyltransferase is a key enzyme in fructo-oligosaccharide production, while the highly demanding conditions of industrial processes may reduce its stability and activity. This study employs sequence alignment structural analysis to target three potential residues (Gln38, Ile39, Cys43) around active center FruSG from Aspergillus niger, mutants with greatly improved activity were obtained through site-directed mutagenesis. The Km values C43N Q38Y were, respectively, reduced 60.8 93.1%...
Cembratriene-ol is a good biodegradable biopesticide ingredient with future potential applications in the field of sustainable agriculture. monocyclic diterpenoid compound that synthesized only trichome gland Nicotiana plants. In this study, geranylgeranyl diphosphate synthase gene ggpps from Taxus canadensis and cbts*Δp were heterologously expressed Saccharomyces cerevisiae W303-1A to successfully synthesize cembratriene-ol. The titer cembratriene-ol was increased by 1.84-fold compared...
Metabolic engineering of microorganisms for terpenoid biosynthesis is a green and sustainable alternative to plant extraction chemical synthesis methods producing plant-derived natural products. The compartmentalization pathways or...
The stink bug, Eocanthecona furcellata Wolff (Hemiptera: Pentatomidae) shows significant promise as a biological control agent against lepidopteran pests in tropical and subtropical areas. However, securing suitable food remains persistent issue for the mass rearing of this beneficial insect. To understand how different feeding strategies impact reproductive success, we evaluated their effects on critical life table parameters ovary development using range options: natural prey Spodoptera...
Background Stem tissue structures are the basis of stem function and essential for maintaining normal physiological metabolism aquatic plants. Water sediment conditions important factors affecting functional characteristics Brassenia schreberi . Due to pollution other water issues caused by human activities, natural habitat population size B. have dramatically decreased. Understanding responses is key its scientific conservation management. Objectives methods This study selected Beihai...
High thermostability is required for alkaline α-amylases to maintain high catalytic activity under the harsh conditions used in textile production. In this study, we attempted improve of an α-amylase from Alkalimonas amylolytica through silico rational design and systems engineering disulfide bridges domain. Specifically, 7 residue pairs (P35-G426, Q107-G167, G116-Q120, A147-W160, G233-V265, A332-G370, R436-M480) were chosen as targets bridge formation, respective residues replaced with...
ABSTRACT This work aims to improve the oxidative stability of alkaline amylase from Alkalimonas amylolytica through structure-based site-directed mutagenesis. Based on an analysis tertiary structure, five methionines (Met 145, Met 214, 229, 247, and 317) were selected as mutation sites individually replaced with leucine. In presence 500 mM H 2 O at 35°C for 5 h, wild-type enzyme M145L, M214L, M229L, M247L, M317L mutants retained 10%, 28%, 46%, 72%, 43% original activity, respectively....
Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete proteins to extracellular space. Here, secretion was enhanced in E. by deleting two d,d-carboxypeptidase genes (dacA dacB, single double deletions) perturb cell wall peptidoglycan network. Deletion dacA dacB accumulation intracellular soluble affected morphology, resulting a more irregular shape appearance transparent bulges....
Abstract Background Alkaline α-amylases have potential applications for hydrolyzing starch under high pH conditions in the and textile industries as ingredients detergents automatic dishwashers laundries. While alkaline α-amylase gains increased industrial interest, yield of from wild-type microbes is low, combination genetic engineering process optimization necessary to achieve overproduction α-amylase. Results The gene Bacillus alcalophilus JN21 (CCTCC NO. M 2011229) was cloned expressed...
ABSTRACT In this study, we constructed and expressed six fusion proteins composed of oligopeptides attached to the N terminus alkaline α-amylase (AmyK) from Alkalimonas amylolytica . The had various effects on functional structural characteristics AmyK. AmyK-p1, protein containing peptide 1 (AEAEAKAKAEAEAKAK), exhibited improved specific activity, catalytic efficiency, stability, thermal oxidative stability compared with Compared AmyK, activity constant ( k cat ) AmyK-p1 were increased by...
Alkaline amylase has significant potential for applications in the textile, paper and detergent industries, however, low yield of which cannot meet requirement industrial application. In this work, a novel ARTP mutagenesis-screening method fermentation optimization strategies were used to significantly improve expression level recombinant alkaline B. subtilis 168. The activity mutant 168 mut-16# strain was 1.34-fold greater than that wild-type, highest specific production rate improved from...