The isolation of a number strains infectious bursal disease (IBD) virus from fowl, turkeys and ducks is described. These isolates could be grouped into two serotypes using the neutralisation test. It proposed that cell culture adapted vaccine strain fowl should prototype for serotype 1 TY89 isolate turkey 2. in 2 consisted an antigenically homogeneous group viruses fowl. However, within 1, which represented ducks, some showed only 30% cross reaction with strain. If protection mirrors...

Chicken anemia virus (CAV) causes cytopathogenic effects in chicken thymocytes and cultured transformed mononuclear cells via apoptosis. Early after infection of cells, the CAV-encoded protein VP3 exhibits a finely granular distribution within nucleus. At later stage infection, forms aggregates. this point, cell becomes apoptotic cellular DNA is fragmented condensed. By immunogold electron microscopy was shown to be associated with structures. In vitro, expression induced apoptosis...

The complete nucleotide sequence (1759 nt) of the ssDNA genome porcine circovirus (PCV) was determined from a cloned dsDNA replicative form isolated PCV-infected cells. Sequence analysis detected no significant nucleic acid or protein similarity with another animal circovirus, chicken anaemia virus (CAV) but, surprisingly, highest obtained between product largest predicted PCV ORF (ORF1; encoding potential 35·7 kDa) and putative encoded by plant banana bunchy top (BBTV). High also other...

Chicken anaemia agent (CAA) was purified using differential centrifugation and successive cycles of equilibrium density gradient sucrose CsCl. The purification method dependent on the use an antigen-detecting ELISA based a CAA-specific monoclonal antibody. Virus particles banded at 1.33 to 1.34 g/ml in CsCl measured 23.5 +/- 0.8 nm diameter. Purified preparations contained one major polypeptide (Mr 50,000) single-stranded, circular DNA (2.3 kb). CAA shares some biochemical characteristics...

SUMMARY A virus, designated 132 was isolated from the faeces of chickens in chick embryo liver cell cultures. The morphology and morphogenesis virus were indistinguishable that rotaviruses. nucleic acid had nuclease resistance double-stranded RNA, separated by polyacrylamide gel electrophoresis into 11 segments with mol. wt. ranging 2.07 × 106 to 0.20 106. SPF susceptible oral infection which replicated villous epithelial cells small intestine. therefore a rotavirus morphological,...

A syndrome causing depressed egg production is described. It characterised either by a failure to attain predicted targets or fall in numbers. The depression can reach 30% and it may not return normal. For short period the eggs produced are smaller, lose colour, have poor shell strength many soft shelled laid. birds remain apparently healthy there marked age incidence, with most flocks starting this at 29-31 weeks of age. This has been recently recorded Netherlands, but seen before Northern...

Antigenic characterisation of two highly virulent virus isolates from outbreaks Newcastle disease on closely connected farms in County Monaghan, Republic Ireland, 1990 showed the viruses to be indistinguishable but unlike other so far tested. However, they appeared antigenically closest avirulent isolated waterfowl several countries and chickens Northern Ireland 1986. Despite antigenic differences, vaccinated with a live commercial Hitchner B1 vaccine were protected against intramuscular...

Some important biological and physico-chemical characteristics of porcine circovirus are reported. These include a study on the host distribution in nature, levels colostrum-derived antibodies piglets from sero-positive sows susceptibility range cell cultures to infection with this virus. The results haemagglutination studies, resistance pH 3, chloroform heat also reported as comparative buoyant densities sedimentation coefficients chicken anaemia

Summary A serological survey for antibody to chicken anaemia agent (CAA) was carried out by indirect immunofluorescence. Antibody CAA widespread in broiler breeders and parent commercial layers the UK. also detected five of 11 specific pathogen‐free (SPF) flocks tested; positive included those being used vaccine production or as suppliers SPF eggs. No turkey duck sera. longitudinal two breeder showed that maternal persisted until about 3 weeks age. Both began seroconvert at 8 9 majority...

To evaluate the economic effects of subclinical chicken anemia agent (CAA) infection on broiler performance, clinically normal flocks were grouped into two categories: A) in which none 10 birds sampled at slaughter had antibody to CAA, and B) six or more similarly CAA antibody. Production performance parameters 25 each category compared. No statistically significant differences found between A B major production such as sex, feed manufacturer, type litter, stocking density, age slaughter....

Six isolates of rotavirus were made from turkeys and two chickens. Three these required trypsin treatment for isolation serial passage in cell cultures. The remainder isolated without treatment. Most viruses chick embryo liver cultures the faeces birds aged under 1 week. In six eight instances, was associated with enteric disturbance, characterised by signs such as diarrhoea, poor or abnormal appetite, abnormally fluid gaseous intestinal contents increased mortality. Cross immunofluorescence...

A serological investigation was undertaken of flocks with depressed egg production. No obvious correlation found between antibody to adenovirus, infectious bronchitis virus or bursal disease and this syndrome. detected Newcastle virus, Mycoplasma gallisepticum in most cases synoviae. relationship the development a haemagglutinating 127, retrospective survey fowl sera for 127 indicated first evidence its presence Northern Ireland June 1976. If is aetiological agent syndrome, then it appears...

Detection systems for chicken anemia virus (CAV) antigen in paraffin sections were evaluated. Mouse monoclonal antibodies to CAV used conjunction with an avidin-biotin-peroxidase-complex detection system gave best results. Immunoreactivity of was markedly affected by fixation. Fixation neutral buffered formalin 6 hours Use decalcifying fluid containing formic acid eliminated immunoreactivity CAV, whereas use EDTA solution did not. In a sequential study, and lesions first detected bone...

Complementary oligonucleotide primers which flank a 675-bp DNA fragment encompassing part of the putative gene for capsid protein chicken anemia virus (CAV) were used enzymatic amplification CAV by polymerase chain reaction (PCR). Application dot blot hybridization assay using 32P-labeled cloned probe allowed PCR product amplified from as little 0.1 fg target sequence to be detected. When it was amplification, extracted thymus tissue guanidine isothiocyanate-based method proved more...