A5006938860- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Animal Vocal Communication and Behavior
- Neurological disorders and treatments
- Animal Behavior and Reproduction
- Cellular transport and secretion
- Monoclonal and Polyclonal Antibodies Research
- Advanced NMR Techniques and Applications
- Marine animal studies overview
- T-cell and B-cell Immunology
- Genetic diversity and population structure
- Plant and animal studies
- Veterinary Equine Medical Research
- Advanced Biosensing Techniques and Applications
- Neuroscience and Neuropharmacology Research
- Advanced Neuroimaging Techniques and Applications
- Nanofabrication and Lithography Techniques
- Genomics and Phylogenetic Studies
- Nuclear Receptors and Signaling
- Inorganic and Organometallic Chemistry
- Ruminant Nutrition and Digestive Physiology
- Lipid Membrane Structure and Behavior
- Chromosomal and Genetic Variations
- Labor Movements and Unions
- Fish Ecology and Management Studies
Tarleton State University
2025
Queen Mary University of London
2014-2024
Clemson University
2021-2024
Texas A&M University
1993-2023
Hôpital Necker-Enfants Malades
2023
Hôpital de la Conception
2022
University of Illinois Urbana-Champaign
2004-2014
Rockefeller University
1992-2008
Urbana University
2008
Goodwin College
1997-2006
Abstract High-quality and complete reference genome assemblies are fundamental for the application of genomics to biology, disease, biodiversity conservation. However, such available only a few non-microbial species 1–4 . To address this issue, international Genome 10K (G10K) consortium 5,6 has worked over five-year period evaluate develop cost-effective methods assembling highly accurate nearly genomes. Here we present lessons learned from generating 16 that represent six major vertebrate...
α-Synuclein is a highly conserved presynaptic protein of unknown function. A mutation in the has been causally linked to Parkinson's disease humans, and normal an abundant component intraneuronal inclusions (Lewy bodies) characteristic disease. also precursor intrinsic extracellular plaques Alzheimer's The α-synuclein sequence largely composed degenerate 11-residue repeats reminiscent amphipathic α-helical domains exchangeable apolipoproteins. We hypothesized that should associate with...
The genome of the zebra finch — a songbird and model for study vertebrate brain, behaviour evolution has been sequenced. Its comparison with chicken genome, only other bird available, shows that genes neural function implicated in cognitive processing song have rapidly evolving lineage. also vocal communication engages much brain transcriptome identifies potential integrator microRNA signals linked to communication. studying Comparison are Moreover, brain. is an important organism several...
Synucleins are small highly conserved proteins in vertebrates, especially abundant neurons and typically enriched at presynaptic terminals. Three genes humans produce closely related synuclein proteins, all of which share a large amphipathic domain capable reversible binding to lipid vesicles. Alpha has been specifically implicated neurodegenerative disease. Two point mutations genetically linked familial Parkinson's disease, alpha appears form the major fibrillary component Lewy bodies....
α-Synuclein has been centrally implicated in neurodegenerative disease, and a normal function developmental synaptic plasticity suggested by studies songbirds. A variety of observations suggest the protein partitions between membrane cytosol, behavior apparently conferred conserved structural similarity to exchangeable apolipoproteins. Here we show that capacity bind lipids is broadly distributed across exons 3, 4, 5 (encoding residues 1–102). Binding phosphatidylserine-containing vesicles...
A mutation in the α-synuclein gene has recently been linked to some cases of familial Parkinson's disease (PD). We characterized expression this presynaptic protein midbrain, striatum, and temporal cortex control, PD, dementia with Lewy bodies (DLB) brain. Control brain showed punctate pericellular immunostaining. PD demonstrated immunoreactivity nigral bodies, pale abnormal neurites. Rare neuronal soma were immunoreactive for α-synuclein. DLB these findings as well cortical CA2-3 These...
The researchers proposed that the phenomenological experience of work is multifaceted and simultaneously considering combined effects different aspects holds considerable promise for increasing understanding turnover intentions other organizationally relevant outcomes. Research in subjective well-being areas suggests there are 3 important work: attainment values, attitudes, moods. It was hypothesized found relationship between job satisfaction jointly moderated by value positive mood. nature...
Detergent-stable multimers of alpha-synuclein have been found specifically in the brains patients with Parkinson's disease and other neurodegenerative diseases. Here we show that recombinant forms vitro upon exposure to vesicles containing certain polyunsaturated fatty acid (PUFA) acyl groups, including arachidonoyl docosahexaenoyl. This process occurs at physiological concentrations much faster than aqueous solution. PUFA-induced aggregation involves physical association vesicle surface via...
In this study, we hypothesized that a nurse's exposure to acquired immunodeficiency syndrome (AIDS) patients as part of the work role is positively associated with distress indexed by negative mood at work. Given expected relation, sought identify factors might reduce effects caring for AIDS on nurses. We predicted both organizational and social support would moderate relationship between extent mood, being strongest when low weakest high. The results tests among sample 256 nurses supported...
A novel and highly conserved presynaptic protein has been independently described in rodents (synuclein/SYN-1), songbirds (synelfin), humans (the precursor of the non-A beta component senile plaques, NACP); a fragment latter detected plaques Alzheimer's disease (AD). We characterized expression NACP human AD non-AD brain. subcellular fractionation study demonstrated that was mainly localized to cytosolic fractions temporal cortex. also detectable various membrane vesicular fractions,...
α-Synuclein (AS) fibrils are the main protein component of Lewy bodies, pathological hallmark Parkinson's disease and other related disorders. AS forms helices that bind phospholipid membranes with high affinity, but no atomic level data for aggregation in presence lipids is yet available. Here, we present direct evidence a conversion from α-helical conformation to β-sheet anionic vesicles their absence. We have trapped intermediate states throughout fibril formation pathways examine...
Abstract High-quality and complete reference genome assemblies are fundamental for the application of genomics to biology, disease, biodiversity conservation. However, such only available a few non-microbial species 1–4 . To address this issue, international Genome 10K (G10K) consortium 5,6 has worked over five-year period evaluate develop cost-effective methods assembling most accurate genomes date. Here we summarize these developments, introduce set quality standards, present lessons...
Modifications to the gene encoding human α-synuclein have been linked development of Parkinson's disease. The highly conserved structure suggests a functional interaction with membranes, and several lines evidence point role in vesicle-related processes within nerve terminals. Using recombinant fusions α-synuclein, including new genetic tags developed for correlated light microscopy electron (the tetracysteine-biarsenical labeling system or fluorescent protein microscopy, MiniSOG), we...
Abstract Background Songbirds hold great promise for biomedical, environmental and evolutionary research. A complete draft sequence of the zebra finch genome is imminent, yet a need remains application genomic resources within research community traditionally focused on ethology neurobiological methods. In response, we developed core set tools novel collaborative strategy to probe gene expression in diverse songbird species natural contexts. Results We end-sequenced cDNAs from brain...
An efficient route for delivering specific proteins and peptides into neurons could greatly accelerate the development of therapies various diseases, especially those involving intracellular defects such as Parkinson disease. Here we report novel use polybutylcyanoacrylate nanoparticles delivery intact, functional neuronal cell lines. Uptake these particles is primarily dependent on endocytosis via low density lipoprotein receptor. The are rapidly turned over display minimal toxicity to...
The disruption of α-synuclein (α-syn) homeostasis in neurons is a potential cause Parkinson's disease, which manifested pathologically by the appearance α-syn aggregates, or Lewy bodies. Treatments for neurological diseases are extremely limited. To study use gold nanoparticles (Au NPs) to limit misfolding, binding and orientation on Au NPs were investigated. α-Syn was determined interact with 20 90 nm via multilayered adsorption: strong electrostatic interaction between hard corona weaker...
Parkinson's disease is associated with multiplication of the α-synuclein gene and abnormal accumulation protein. In animal models, overexpression broadly impairs synaptic vesicle trafficking. However, exact steps trafficking pathway affected by excess underlying molecular mechanisms remain unknown. Therefore we acutely increased synuclein levels at a vertebrate synapse performed detailed ultrastructural analysis effects on presynaptic membranes. At stimulated synapses (20 Hz), caused loss...