A5063450635- Microbial Community Ecology and Physiology
- DNA Repair Mechanisms
- Photosynthetic Processes and Mechanisms
- Paleontology and Stratigraphy of Fossils
- Enzyme Structure and Function
- Geology and Paleoclimatology Research
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Genomics and Chromatin Dynamics
- Intraocular Surgery and Lenses
- Biochemical and Structural Characterization
- DNA and Nucleic Acid Chemistry
- Ocular Surface and Contact Lens
- Isotope Analysis in Ecology
- Biological Activity of Diterpenoids and Biflavonoids
- Connective tissue disorders research
- Porphyrin Metabolism and Disorders
- Epigenetics and DNA Methylation
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Carbohydrate Chemistry and Synthesis
- Biotin and Related Studies
- RNA modifications and cancer
- Acute Lymphoblastic Leukemia research
- Atmospheric and Environmental Gas Dynamics
Lawrence Berkeley National Laboratory
2022-2023
University of California, Davis
2017-2023
University of Minnesota
2016
Oligomerization is a core structural feature that defines the form and function of many proteins. Most proteins molecular complexes; however, there remains dearth diversity-driven studies investigating evolutionary trajectory these assemblies. Ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) one such enzyme adopts multiple assemblies, although origins distribution its different oligomeric states remain cryptic. Here, we retrace evolution ancestral extant II RuBisCOs, revealing...
The history of Earth’s carbon cycle reflects trends in atmospheric composition convolved with the evolution photosynthesis. Fortunately, key parts have been recorded isotope ratios sedimentary rocks. dominant model used to interpret this record as a proxy for ancient CO 2 is based on fractionations modern photoautotrophs, and longstanding questions remain about how their might impacted record. Therefore, we measured both biomass (ε p ) enzymatic Rubisco cyanobacterial strain ( Synechococcus...
Higher expression of the human DNA repair enzyme MUTYH has previously been shown to be strongly associated with reduced survival in a panel 24 lymphoblastoid cell lines exposed alkylating agent <i>N</i>-methyl-<i>N</i>′-nitro-<i>N</i>-nitrosoguanidine (MNNG). The molecular mechanism MUTYH-enhanced MNNG cytotoxicity is unclear, because well-established role oxidative lesions. Here, we show mouse embryonic fibroblasts (MEFs) that this MNNG-dependent phenotype does not involve damage and occurs...
Form I rubiscos evolved in Cyanobacteria ≥ 2.5 billion years ago and are enzymatically unique due to the presence of small subunits (RbcS) capping both ends an octameric large subunit (RbcL) rubisco assembly form a hexadecameric (L
Evidence that surface membrane glycoproteins of polymorphonuclear leukocytes (PMN) are involved in stimulus-response coupling prompted us to examine effects on these cells various plant lectins. We have found wheat germ agglutinin (WGA) (1.0 microgram/ml) completely, specifically, and irreversibly inhibits directed migration (chemotaxis) human PMN toward the synthetic peptide, N-formylmethionyl-leucyl-phenylalanine (FMLP) (0.1 100 nM). This effect WGA was not shared by subagglutinating...
The important industrial and environmental carcinogen 1,3-butadiene (BD) forms a range of adenine adducts in DNA, including N6-(2-hydroxy-3-buten-1-yl)-2'-deoxyadenosine (N6-HB-dA), 1,N6-(2-hydroxy-3-hydroxymethylpropan-1,3-diyl)-2'-deoxyadenosine (1,N6-HMHP-dA), N6,N6-(2,3-dihydroxybutan-1,4-diyl)-2'-deoxyadenosine (N6,N6-DHB-dA). If not removed prior to DNA replication, these lesions can contribute A → T G mutations commonly observed following exposure BD its metabolites. In this study,...
Abstract The history of Earth’s carbon cycle reflects trends in atmospheric composition convolved with the evolution photosynthesis. Fortunately, key parts have been recorded isotope ratios sedimentary rocks. dominant model used to interpret this record as a proxy for ancient CO 2 is based on fractionations modern photoautotrophs, and longstanding questions remain about how their might impacted record. We tested intersection environment by measuring both biomass (ε p ) enzymatic Rubisco...
Abstract Form I rubiscos evolved in Cyanobacteria ≥2.5 billion years ago and are enzymatically unique due to the presence of small subunits (RbcS) that cap both ends an octameric large subunit (RbcL) rubisco assembly form a hexadecameric (L 8 S ) holoenzyme. Although RbcS was previously thought be integral stability, recent discovery closely related sister clade (Form I’; L demonstrates enzyme complex assembles without (Banda et al. 2020). Rubisco also displays kinetic isotope effect (KIE)...