A5046390613- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Geology and Paleoclimatology Research
- Protist diversity and phylogeny
- Soil Carbon and Nitrogen Dynamics
- Photosynthetic Processes and Mechanisms
- Paleontology and Stratigraphy of Fossils
- Isotope Analysis in Ecology
- Atmospheric and Environmental Gas Dynamics
- Age of Information Optimization
- Aquatic Ecosystems and Phytoplankton Dynamics
- Soil and Water Nutrient Dynamics
- Botanical Research and Applications
- Microbial Metabolic Engineering and Bioproduction
- Marine and environmental studies
- Coastal wetland ecosystem dynamics
- Wastewater Treatment and Nitrogen Removal
- Protein Structure and Dynamics
- Metabolomics and Mass Spectrometry Studies
University of California, Berkeley
2024-2025
Los Alamos National Laboratory
2024
California Institute of Technology
2022-2024
Planetary Science Institute
2023-2024
Pasadena City College
2023
University of Southern California
2016
Abstract Rubisco is the primary CO 2 -fixing enzyme of biosphere 1 , yet it has slow kinetics . The roles evolution and chemical mechanism in constraining its biochemical function remain debated 3,4 Engineering efforts aimed at adjusting parameters rubisco have largely failed 5 although recent results indicate that functional potential a wider scope than previously known 6 Here we developed massively parallel assay, using an engineered Escherichia coli 7 which activity coupled to growth,...
Abstract While lignin geochemistry has been extensively investigated in the Amazon River, little is known about distribution and dynamics within deep, stratified river channels or its transformations soils prior to delivery rivers. We characterized phenols soils, particulate organic matter (POM), dissolved (DOM) across a 4 km elevation gradient Madre de Dios River system, Peru, as well marine sediments investigate source‐to‐sink evolution of lignin. In we found more oxidized horizons...
The history of Earth’s carbon cycle reflects trends in atmospheric composition convolved with the evolution photosynthesis. Fortunately, key parts have been recorded isotope ratios sedimentary rocks. dominant model used to interpret this record as a proxy for ancient CO 2 is based on fractionations modern photoautotrophs, and longstanding questions remain about how their might impacted record. Therefore, we measured both biomass (ε p ) enzymatic Rubisco cyanobacterial strain ( Synechococcus...
Cyanobacteria are highly abundant in the marine photic zone and primary drivers of conversion inorganic carbon into biomass. To date, all studied cyanobacterial lineages encode fixation machinery relying upon form I Rubiscos within a CO
Form I rubiscos evolved in Cyanobacteria ≥ 2.5 billion years ago and are enzymatically unique due to the presence of small subunits (RbcS) capping both ends an octameric large subunit (RbcL) rubisco assembly form a hexadecameric (L
Abstract The history of Earth’s carbon cycle reflects trends in atmospheric composition convolved with the evolution photosynthesis. Fortunately, key parts have been recorded isotope ratios sedimentary rocks. dominant model used to interpret this record as a proxy for ancient CO 2 is based on fractionations modern photoautotrophs, and longstanding questions remain about how their might impacted record. We tested intersection environment by measuring both biomass (ε p ) enzymatic Rubisco...
Nitrous oxide (N 2 O), a potent greenhouse gas, can be generated by multiple biological and abiotic processes in diverse contexts. Accurately tracking the dominant sources of N O has potential to improve our understanding fluxes from soils as well inform diagnosis human infections. Isotopic “Site Preference” (SP) values have been used toward this end, bacterial fungal nitric reductases (NORs) produce with different isotopic fingerprints, spanning large range. Here, we show that...
Abstract Cyanobacteria are highly abundant in the marine photic zone and primary drivers of conversion inorganic carbon to biomass. To date, all studied Cyanobacterial lineages encode fixation machinery hinged upon form I rubisco enzymes within a CO 2 -concentrating carboxysome. Here, we report that AMZ IB lineage Prochlorococcus from global oxygen deficient zones (ODZs) harbor both II enzymes, latter which typically non-carboxysomal possess biochemical properties tuned towards low...
Abstract Plant litter decomposition is a major nutrient input to terrestrial ecosystems that primarily driven by microorganisms. Litter quality considered key drive of decomposition; however, human-induced global disturbance like nitrogen deposition and increasing extreme precipitation events will shift availability during decomposition. Little known about how shifting impact dissolved organic matter concentrations microbially carbon cycling are critical soil formation. This study...
Nitrous oxide (N
Abstract Plant litter decomposition is a major nutrient input to terrestrial ecosystems that primarily driven by microorganisms. Litter results in flow of dissolved organic carbon (DOC) links above-ground below-ground microbial processes. expected be altered human-induced global disturbances—specifically nitrogen deposition and intensity frequency precipitation events—but little known about impacts on the mobile pool DOC. This study investigated effect simulated increased events...
Abstract Form I rubiscos evolved in Cyanobacteria ≥2.5 billion years ago and are enzymatically unique due to the presence of small subunits (RbcS) that cap both ends an octameric large subunit (RbcL) rubisco assembly form a hexadecameric (L 8 S ) holoenzyme. Although RbcS was previously thought be integral stability, recent discovery closely related sister clade (Form I’; L demonstrates enzyme complex assembles without (Banda et al. 2020). Rubisco also displays kinetic isotope effect (KIE)...