A5032367985- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Plant biochemistry and biosynthesis
- Photosynthetic Processes and Mechanisms
- Microbial Natural Products and Biosynthesis
- Microbial Metabolism and Applications
- Aquatic Ecosystems and Phytoplankton Dynamics
- Microbial Fuel Cells and Bioremediation
- Protein Structure and Dynamics
- Catalysis for Biomass Conversion
University of California, Berkeley
2022-2025
Joint BioEnergy Institute
2022-2024
Lawrence Berkeley National Laboratory
2022-2024
Abstract Rubisco is the primary CO 2 -fixing enzyme of biosphere 1 , yet it has slow kinetics . The roles evolution and chemical mechanism in constraining its biochemical function remain debated 3,4 Engineering efforts aimed at adjusting parameters rubisco have largely failed 5 although recent results indicate that functional potential a wider scope than previously known 6 Here we developed massively parallel assay, using an engineered Escherichia coli 7 which activity coupled to growth,...
Cyclopropane-functionalized hydrocarbons are excellent fuels due their high energy density. However, the organic synthesis of these molecules is challenging. In this work, we produced polycyclopropanated fatty acids in bacteria. These can be converted into renewable for energy-demanding applications such as shipping, long-haul transport, aviation, and rocketry. We explored chemical diversity encoded thousands bacterial genomes to identify repurpose naturally occurring cyclopropanated...
Abstract Rubisco is the primary CO 2 fixing enzyme of biosphere yet has slow kinetics. The roles evolution and chemical mechanism in constraining sequence landscape rubisco remain debated. In order to map function, we developed a massively parallel assay for using an engineered E. coli where function coupled growth. By assaying >99% single amino acid mutants across concentrations, inferred velocity affinity thousands substitutions. We identified many highly conserved positions that...
Abstract Engineering the heterologous expression of polyketide synthases (PKSs) in Escherichia coli has led to production various valuable natural and unnatural products. However, limited availability malonyl-CoA (M-CoA) E. remains a significant impediment efficient overproduction. In this study, engineered strains were developed augment M-CoA levels for enhanced by introducing malonate transport CoA ligation pathway. Additionally, control over pool was achieved disrupting endogenous pathway...
Abstract Corynebacterium glutamicum is a promising host for production of valuable polyketides. Propionate addition, strategy known to increase polyketide by increasing intracellular methylmalonyl-CoA availability, causes growth inhibition in C. . The mechanism this was unclear prior our work. Here we provide evidence that accumulation propionyl- and induces We then show can be relieved introducing methylmalonyl-CoA-dependent synthases. With germicidin as an example, used adaptive laboratory...