A5047780037- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Plant and Fungal Interactions Research
- Plant Disease Resistance and Genetics
- Insect symbiosis and bacterial influences
- Nematode management and characterization studies
- Insect-Plant Interactions and Control
- Plant Parasitism and Resistance
- Mosquito-borne diseases and control
- Agricultural pest management studies
- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Legume Nitrogen Fixing Symbiosis
- Cassava research and cyanide
- Plant pathogens and resistance mechanisms
- Plant and animal studies
- Genetic and Environmental Crop Studies
- Plant Pathogens and Resistance
- Agronomic Practices and Intercropping Systems
- CRISPR and Genetic Engineering
- Plant Pathogens and Fungal Diseases
- Insect and Arachnid Ecology and Behavior
- Botanical Research and Chemistry
- Plant Disease Management Techniques
- Insect and Pesticide Research
Department of Primary Industries and Regional Development
2017-2025
Agriculture Victoria
2024
Government of Western Australia
2017-2024
Agriculture and Food
2010-2018
Department of Agriculture and Food Western Australia
2015-2018
The University of Western Australia
2011-2017
Commonwealth Scientific and Industrial Research Organisation
2017
National University of East Timor
2017
Murdoch University
2010
Cassava brown streak disease is caused by two devastating viruses, virus (CBSV) and Ugandan cassava (UCBSV) which are frequently found infecting cassava, one of sub-Saharan Africa's most important staple food crops. Each year these viruses cause losses up to $100 million USD can leave entire families without their primary source, for an year. Twelve new whole genomes, including seven CBSV five UCBSV were uncovered in this research, doubling the genomic sequences available public domain...
In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with MinION and MinIT mobile sequencing devices Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The extracts using a cocktail of thermophilic proteinases cell wall-degrading enzymes, thermo-responsive extractor cartridges temperature control unit. This closed system delivers purified no cross-contamination. is newly released data...
Abstract Cassava is a major staple food for about 800 million people in the tropics and sub-tropical regions of world. Production cassava significantly hampered by brown streak disease (CBSD), caused virus (CBSV) Ugandan (UCBSV). The suppressing yields eastern Africa at an alarming rate. Previous studies have documented that CBSV more devastating than UCBSV because it readily infects both susceptible tolerant cultivars, resulting greater yield losses. Using whole genome sequences from NGS...
Next generation sequencing is quickly emerging as the go-to tool for plant virologists when whole virus genomes, and undertaking metagenomic studies new discoveries. This study aims to compare genomic biological properties of Bean yellow mosaic (BYMV) (genus Potyvirus), isolates from Lupinus angustifolius plants with black pod syndrome (BPS), systemic necrosis or non-necrotic symptoms, two other species. When one Clover vein (ClYVV) Potyvirus) 22 BYMV were sequenced on Illumina HiSeq2000,...
Biological and whole genome properties were compared between eight historical European (1943–1984) five Australian (2003–2012) Potato virus Y ( PVY ) isolates. Based on eliciting hypersensitivity genes Nc , Ny or Nz the former belonged to biological strain groups C CT CRM 1), O CRN KE RS Z CM 2, DS ). The latter inoculated differential other potato cultivars, tobacco tomato. Two BL DEL 3), one ATL KIP 1) suggested group D which elicited putative gene Nd . Tomato isolate CN 1 (and unsequenced...
Sequencing of a Polymerase Chain Reaction product (amplicon) is called amplicon sequencing. Amplicon sequencing allows for reliable identification an organism by amplifying, sequencing, and analysing single conserved marker gene or DNA barcode. As this approach generally involves gene, it light-weight protocol compared to multi-locus whole genome diagnostic purposes; yet considerably reliable. Therefore, Sanger-based high-quality widely deployed species high-throughput biosecurity...
Biological characteristics of 11 Potato virus S (PVS) isolates from three cultivated potato species (Solanum spp.) growing in five Andean countries and 1 Scotland differed virulence depending on isolate host species. Nine infected Chenopodium quinoa systemically but two others the Scottish remained restricted to inoculated leaves; therefore, they belonged biologically defined strains PVSA PVSO, respectively. When nine wild were inoculated, most developed symptomless systemic infection...
Cassava is the main staple food for over 800 million people globally. Its production in eastern Africa being constrained by two devastating Ipomoviruses that cause cassava brown streak disease (CBSD); virus (CBSV) and Ugandan (UCBSV), with up to 100% yield loss smallholder farmers region. To date, vector studies have not resulted reproducible highly efficient transmission of CBSV UCBSV. Most used Bemisia tabaci (whitefly), but a maximum 41% U/CBSV efficiency has been documented this vector....
<ns4:p>Crop losses due to viral diseases and pests are major constraints on food security income for millions of households in sub-Saharan Africa (SSA). Such can be reduced if plant correctly diagnosed identified early. Currently, accurate diagnosis definitive identification viruses their vectors SSA mostly relies standard PCR next generation sequencing technologies (NGS). However, it take up 6 months before results generated using these approaches available. The long time taken detect or...
Forty-seven potato virus A (PVA) isolates from Europe, Australia, and South America’s Andean region were subjected to high-throughput sequencing, 46 complete genomes Europe (n = 9), Australia 2), the Andes 35) obtained. These 17 other gave alignments of 63 open reading frames 9,180 nucleotides long; 9 recombinants. The nonrecombinants formed three tightly clustered, almost equidistant phylogroups; comprised 14 Peruvian isolates; W 37 in Peru, Argentina, elsewhere world; T contained tamarillo...
Wheat streak mosaic virus (WSMV) infection and infestation with its wheat curl mite (WCM; Aceria tosichella) vector were investigated in crops at two sites the low-rainfall zone of central grainbelt south-west Australia. In 2006 outbreak, after a preceding wet summer autumn, high WCM populations total WSMV throughout crop associated presence abundant grasses self-sown ‘volunteer’ plants before sowing field that became affected. Wind strength direction had major effect on spread by to...
In glasshouse experiments, Zucchini yellow mosaic virus (ZYMV) was transmitted from infected to healthy zucchini (Cucurbita pepo) plants by direct contact when leaves were rubbed against each other, crushed, or trampled, and, a lesser extent, on ZYMV-contaminated blades. When sap with three ZYMV isolates kept at room temperature for up 6 h, it readily. Also, ZYMV-infected applied seven surfaces (cotton, plastic, leather, metal, rubber vehicle tire, rubber-soled footwear, and human skin) left...
Bean yellow mosaic virus (BYMV), genus Potyvirus, has an extensive natural host range encompassing both dicots and monocots. Its phylogenetic groups were considered to consist of ancestral generalist group six specialist derived from this during plant domestication. Recombination was suggested be playing a role in BYMV's evolution towards specialization. However, subsequent analysis whole genomes, names based on the original hosts isolates within each them no longer supported. Also, nine...
Zucchini yellow mosaic virus (ZYMV) isolates from cucurbit crops growing in northern Australia and East Timor were investigated to establish possible genetic connectivity between crop viruses Southeast Asia. Leaves symptomatic plants of pumpkin (Cucurbita moschata C. maxima), melon (Cucumis melo), zucchini (C. pepo) sampled near Broome, Darwin, Kununurra Australia. cucumber sativus) sent on FTA cards. These samples subjected high-throughput sequencing 15 complete new ZYMV genomic sequences...
Abstract In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with MinION and MinIT mobile sequencing devices Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The extracts using a cocktail of thermophilic proteinases cell wall degrading enzymes, thermo-responsive extractor cartridges temperature control unit. This single-step closed system delivers purified no cross contamination. is...
In this study, metagenomic sequence data was used to investigate the phytoplasma taxonomic diversity in vegetable-growing regions across Australia. Metagenomic sequencing performed on 195 phytoplasma-positive samples, originating either from historic collections ( n =46) or during collection efforts between January 2015 and June 2022 =149). The sampled hosts were classified as crop =155), weed =24), ornamental =7), native plant =6), insect =3) species. Most samples came Queensland =78),...
Turnip mosaic virus (Tu MV ) causes crop losses worldwide. Eight Australian Tu isolates originally obtained from five different species in two plant families were inoculated to 14 belonging four compare their host reactions. They differed considerably virulence Brassicaceae and indicator hosts three other families. The infected most Brassica inoculated, but not Raphanus sativus , usually causing systemic symptoms, so they resembled biological type [B]. Whole genome sequences of seven the had...