A5044013577- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Nematode management and characterization studies
- Cassava research and cyanide
- Agricultural pest management studies
- Banana Cultivation and Research
- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Interactions Research
- Mosquito-borne diseases and control
- Plant and animal studies
- Plant Pathogens and Fungal Diseases
- Insect symbiosis and bacterial influences
- Plant Disease Management Techniques
- Mycorrhizal Fungi and Plant Interactions
- Genetic and Environmental Crop Studies
- Plant Molecular Biology Research
- CRISPR and Genetic Engineering
- Agricultural Innovations and Practices
- Cocoa and Sweet Potato Agronomy
- Transgenic Plants and Applications
Jomo Kenyatta University of Agriculture and Technology
2016-2025
Pan African University Institute for Basic Sciences, Technology and Innovation
2024
University of Eswatini
2022
Institute for Basic Science
2022
Pan-Atlantic University
2022
Practical Action
2022
University of Eldoret
2015
Federal Office for Food and Agriculture
2006
University of Nairobi
2004
In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with MinION and MinIT mobile sequencing devices Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The extracts using a cocktail of thermophilic proteinases cell wall-degrading enzymes, thermo-responsive extractor cartridges temperature control unit. This closed system delivers purified no cross-contamination. is newly released data...
Rice is the most important cereal crop in Kenya coming third after maize and wheat. It forms a very diet for majority of families Kenya. The demand rice has seen dramatic increase over last few years while production remained low. This because been faced by serious constraints notably plant diseases which devastating blast. blast known to cause approximately 60% -100% yield losses. caused an Ascomycete fungus called Magnaporthe Oryzae. aim this study was investigate impact disease on...
Abstract The genetic variability of whitefly ( B emisia tabaci ) species, the vectors cassava mosaic begomoviruses CMB s) in growing areas K enya, T anzania, and U ganda, was investigated through comparison partial sequences mitochondria cytochrome oxidase I (mt COI DNA 2010/11. Two distinct species were obtained including sub‐ S aharan A frica 1 SSA 1), comprising two sub‐clades (I II ), a outh W est ndian O cean Islands SWIO species. Among 1, sub‐clade shared similarity 97.8–99.7% with...
Tomato spotted wilt virus (TSWV) is an important plant that infects a wide range of hosts including weeds making its management difficult. A survey was undertaken to establish the occurrence weed species in tomato production systems Kenya and their role as TSWV vectors. Selected were further evaluated for reaction TSWV, transmission efficiency by Frankliniella occidentalis ability support thrips reproduction. Of 43 identified field, 29 had been reported two non-hosts 11 no record status....
Various modes of edible coating application vary in their coat dispersion and film formation, hence the need to determine most effective mode for cassava. Edible surface coatings have been found be preserving quality various food products. However, there are variations effectiveness among different solutions, optimization concentrations gums used. This study aimed at determining efficient method on cassava postharvest quality. Physiologically mature (variety KME 1) was harvested divided into...
Cassava is an important root crop that produced by smallholder farmers across Sub-Saharan Africa. mosaic disease (CMD), which caused a group of cassava begomoviruses (CMBs), one the most devastating diseases cassava. A previous study showed SEGS-1 (sequences enhancing geminivirus symptoms), occur both in genome and as episomes during CMD, can increase CMD severity overcome host resistance. In this report, we examined effects exogenously applied on incidence CMB infection, symptom severity,...
Abstract Rice yellow mottle disease (RYMD) continues to constrain rice production in Africa. virus (RYMV), which belongs the Sobemovirus genus, is causal agent of RYMD. In Kenya, previous studies on RYMV mostly focused western ignoring central and coastal regions, are also important areas for cultivation country. This has resulted incomplete data RYMD outbreaks. study aimed determine prevalence farmers' fields, genetic diversity based coat protein gene. Field surveys were carried out Kenya's...
Sweetpotato (Ipomoea batatas) is a vital crop for overcoming food insecurity in sub-Saharan Africa and its production highest East where yields are high the growing seasons short. This cross-country study assessed farmers' local practices their knowledge of biotic constraints to sweetpotato Uganda, Rwanda, Kenya Tanzania with aim providing empirical data that can ultimately be used enhance these four countries. We collected from 675 households using standardized questionnaire integrated...
Cassava brown streak disease (CBSD) and cassava mosaic (CMD) are two viral diseases that cause severe yield losses in of up to 100%, thereby persistently threatening food income security sub-Saharan Africa. For effective management these diseases, there is a critical need develop deploy varieties with dual resistance CBSD CMD. In this study, we determined the response advanced breeding lines field infection by viruses (CBSVs) begomoviruses (CMBs). This aim helped identifying superior clones...
Summary Four hundred and forty‐eight symptomatic 638 asymptomatic samples were collected from sweet potato fields throughout Kenya analysed serologically using antibodies to Sweet feathery mottle virus (SPFMV), chlorotic stunt (SPCSV), mild (SPMMV), Cucumber mosaic (CMV), fleck (SPCFV), latent (SwPLV), caulimo‐like (SPCaLV), speckling (SPMSV) C‐6 in enzyme‐linked immunosorbent assays (ELISA). Only SPFMV, SPMMV, SPCSV, SPCFV detected. Ninety‐two percent 25% of the plants respectively tested...
The efficiency of cassava brown streak virus (CBSV) transmission by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) and spiraling whitefly (Aleurodicus dispersus) Russell (Hom, was determined. utilized field collected adult populations fed on (allowed 48 h acquisition access feeding period (AAP)) CBSD (cassava disease) symptomatic leaves before transfer onto clean recipient plants. In subsequent experiments, numbers each species were varied per plant to determine the effect rate CBSV...
Cassava is the main staple food for over 800 million people globally. Its production in eastern Africa being constrained by two devastating Ipomoviruses that cause cassava brown streak disease (CBSD); virus (CBSV) and Ugandan (UCBSV), with up to 100% yield loss smallholder farmers region. To date, vector studies have not resulted reproducible highly efficient transmission of CBSV UCBSV. Most used Bemisia tabaci (whitefly), but a maximum 41% U/CBSV efficiency has been documented this vector....
Thrips have been recognized as primary vectors of tomato spotted wilt virus (TSWV) with Frankliniella occidentalis (Pergande) reported the most important and efficient vector, while other species such tabaci Lindeman also include populations that can vector virus. A study was undertaken to establish diversity thrips presence for TSWV in four major production areas Kenya. The cytochrome oxidase 1 (CO1) gene used generate sequences from samples collected tomatoes weeds, phylogenetic analysis...
Mitigation of cassava mosaic disease (CMD) focuses on the introgression resistance imparted by polygenic recessive (CMD1), dominant monogenic (CMD2) and CMD3 loci. The mechanism(s) they impart, however, remain unknown. Two CMD susceptible nine resistant genotypes were inoculated microparticle bombardment with infectious clones African virus Cameroon strain (ACMV-CM) Kenyan K201 East (EACMV KE2 [K201]). Genotypes carrying CMD1 (TMS 30572), CMD2 (TME 3, TME 204 Oko-iyawo) 97/0505) mechanisms...
<ns4:p>Crop losses due to viral diseases and pests are major constraints on food security income for millions of households in sub-Saharan Africa (SSA). Such can be reduced if plant correctly diagnosed identified early. Currently, accurate diagnosis definitive identification viruses their vectors SSA mostly relies standard PCR next generation sequencing technologies (NGS). However, it take up 6 months before results generated using these approaches available. The long time taken detect or...
Cassava brown streak disease (CBSD) was first observed on cassava ( Manihot esculenta ) in Rwanda 2009. In 2014 eight major cassava‐growing districts the country were surveyed to determine distribution and variability of symptom phenotypes associated with CBSD, genetic diversity viruses. Distribution CBSD their combinations varied greatly between districts, cultivars The symptoms leaf alone recorded highest (32.2%) incidence, followed by roots (25.7%), + stem (20.3%), root (10.4%), (5.2%),...
Cassava varieties resistant to cassava mosaic disease (CMD) and brown streak (CBSD) are needed for the food income security of rural poor in eastern southern Africa (ESA). The International Institute Tropical Agriculture led five national breeding programs (Malawi, Mozambique, Kenya, Tanzania Uganda) virus-cleaning exchanging elite germplasm both diseases. This paper documents experiences lessons learned from process. Thirty-one clones (25 elite, two standard four national) were submitted by...
Carica papaya L. is an important fruit crop grown by small- and large-scale farmers in Kenya for local export markets. However, its production constrained ringspot disease (PRSD). The believed to be caused virus (PRSV). Previous attempts detect PRSV plants showing PRSD symptoms, using enzyme-linked immunosorbent assay (ELISA) reverse transcriptase-polymerase chain reaction (RT-PCR) procedures with primers specific PRSV, have not yielded conclusive results. Therefore, the nature of viruses...
BF2P4-5 was isolated from the rhizosphere soil of tomato plants, and its potential to promote plant growth investigated in plants. An vitro test revealed that strain could fix nitrogen, solubilize phosphate potassium, synthesize indole acetic acid. The bacterial identified characterized as a kind Pseudarthrobacter chlorophenolicus based on analysis culture characteristics, physiological biochemical 16S rRNA gene sequence (GenBank accession number OP135548.1). pH 7.0, 15% NaCl, 35 °C...
Genetic underpinnings of host-pathogen interactions in the parasitic plant Striga hermonthica, a root that ravages cereals sub-Saharan Africa, are unclear. We performed comparative transcriptome study on five genotypes sorghum exhibiting diverse resistance responses to S. hermonthica using weighted gene co-expression network analysis (WGCNA). found elicits both basal and effector-triggered immunity-like bona fide pathogen. The response was genotype specific. Some followed salicylic...
Abstract In this case study we successfully teamed the PDQeX DNA purification technology developed by MicroGEM, New Zealand, with MinION and MinIT mobile sequencing devices Oxford Nanopore Technologies to produce an effective point-of-need field diagnostic system. The extracts using a cocktail of thermophilic proteinases cell wall degrading enzymes, thermo-responsive extractor cartridges temperature control unit. This single-step closed system delivers purified no cross contamination. is...