A5062629066- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Animal Virus Infections Studies
- Plant Pathogenic Bacteria Studies
- Plant Disease Resistance and Genetics
- Cocoa and Sweet Potato Agronomy
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Livestock and Poultry Management
- Insect symbiosis and bacterial influences
- Plant Pathogens and Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Insect-Plant Interactions and Control
- Bacteriophages and microbial interactions
- Nematode management and characterization studies
- Agricultural pest management studies
- Gut microbiota and health
- Probiotics and Fermented Foods
- Legume Nitrogen Fixing Symbiosis
- Genetic and phenotypic traits in livestock
- Plant Pathogens and Resistance
- Fecal contamination and water quality
- Plant Parasitism and Resistance
New South Wales Department of Primary Industries
2016-2025
Agriculture Victoria
2019-2023
Australian Export Grains Innovation Centre
2021-2023
Kenya Medical Research Institute
2023
The University of Western Australia
2016-2021
International Livestock Research Institute
2014-2020
ACT Government
2016-2018
Plant Biosecurity Cooperative Research Centre
2016-2018
Department of Agriculture and Food Western Australia
2016-2017
CSIRO Land and Water
2017
Cassava brown streak disease is caused by two devastating viruses, virus (CBSV) and Ugandan cassava (UCBSV) which are frequently found infecting cassava, one of sub-Saharan Africa's most important staple food crops. Each year these viruses cause losses up to $100 million USD can leave entire families without their primary source, for an year. Twelve new whole genomes, including seven CBSV five UCBSV were uncovered in this research, doubling the genomic sequences available public domain...
Maize lethal necrosis is caused by a synergistic co-infection of chlorotic mottle virus (MCMV) and specific member the Potyviridae, such as Sugarcane mosaic (SCMV), Wheat streak (WSMV) or Johnson grass (JGMV). Typical maize symptoms include severe yellowing leaf drying from edges. In Kenya, we detected plants showing typical atypical symptoms. Both groups often tested negative for SCMV ELISA. We used next-generation sequencing to identify viruses associated in Kenya through metagenomics...
Abstract Advances in high throughput omics techniques provide avenues to decipher plant microbiomes. However, there is limited information on how integrated informatics can help deeper insights into plant–microbe interactions a concerted way. Integrating multi-omics datasets transform our understanding of the microbiome from unspecified genetic influences interacting species specific gene-by-gene interactions. Here, we highlight recent progress and emerging strategies crop research review...
Abstract Background The African eggplant (Solanum aethiopicum) is a nutritious traditional vegetable used in many countries, including Uganda and Nigeria. It thought to have been domesticated Africa from its wild relative, Solanum anguivi. S. aethiopicum has routinely as source of disease resistance genes for several Solanaceae crops, melongena. A lack genomic resources meant that breeding lagged behind other crops. Results We assembled 1.02-Gb draft genome aethiopicum, which contained...
Multiple types of solid waste in developing countries is disposed together dumpsites where there interaction between humans, animals and the bacteria waste. To study at dumpsite associated risks, previous studies have focused on culturable, leaving behind a great number unculturable bacteria. This focuses more comprehensive approach to dumpsite. Since site comprised unsorted wastes, qualitative survey was first performed identify variety as this has influence microbial composition. Thus,...
Thrips have been recognized as primary vectors of tomato spotted wilt virus (TSWV) with Frankliniella occidentalis (Pergande) reported the most important and efficient vector, while other species such tabaci Lindeman also include populations that can vector virus. A study was undertaken to establish diversity thrips presence for TSWV in four major production areas Kenya. The cytochrome oxidase 1 (CO1) gene used generate sequences from samples collected tomatoes weeds, phylogenetic analysis...
High-throughput sequencing (HTS) technologies have revolutionized plant virology through simultaneous detection of mixed viral infections. HTS advances uncovered and improved understanding virus biology, ecology, evolution which is vital for disease management. Plant viruses continue to threaten global agricultural productivity strict quarantine measures are essential prevent the introduction spread virulent around world. The gradual decrease in operational costs, including computational...
To examine possible genetic connectivity between crop viruses found in Southeast Asia and Australia, Papaya ringspot virus biotype W (PRSV-W) isolates from cucurbits growing East Timor northern Australia were studied. Timorese samples cucumber (Cucumis sativus) or pumpkin (Cucurbita moschata C. maxima) sent to on FTA cards. These others of pumpkin, rockmelon, honeydew melon melo), watermelon (Citrullus lanatus) one location each northwest, north, northeast subjected high throughput...
A new resistance-breaking strain of Turnip mosaic virus (TuMV) overcomes TuMV resistance genes that currently suppress spread this in Brassica napus crops the Liverpool Plains region eastern Australia. Isolates 12.1 and 12.5 three other isolates pathotypes 1 (NSW-2), 7 (NSW-1), 8 (WA-Ap1) were inoculated to plants 19 B. cultivars one breeding line. All these line proved susceptible but developed only phenotypes with WA-Ap1 or mostly NSW-1 NSW-2. Five different occurred either alone...
Zucchini yellow mosaic virus (ZYMV) isolates from cucurbit crops growing in northern Australia and East Timor were investigated to establish possible genetic connectivity between crop viruses Southeast Asia. Leaves symptomatic plants of pumpkin (Cucurbita moschata C. maxima), melon (Cucumis melo), zucchini (C. pepo) sampled near Broome, Darwin, Kununurra Australia. cucumber sativus) sent on FTA cards. These samples subjected high-throughput sequencing 15 complete new ZYMV genomic sequences...
ABSTRACT We present here the first complete genomic Aphid lethal paralysis virus (ALPV) sequence isolated from cucumber plant RNA East Timor. compare it with two ALPV genome sequences China, and one each Israel, South Africa, United States. It most closely resembled Chinese isolate LGH genome.
Sweet potato feathery mottle virus (SPFMV) and C (SPVC) isolates from sweetpotato were studied to examine genetic connectivity between viruses Australia Southeast Asia. East Timorese samples sent on FTA cards. Shoot tuberous root collected in planted the glasshouse, scions graft inoculated Ipomoea setosa plants. Symptoms infected I. plants recorded. RNA extracts cards leaf subjected high-throughput sequencing (HTS). Complete genomic sequences (CS) of SPFMV SPVC (11 each) obtained by HTS,...
Globally, high-throughput sequencing (HTS) has been used for virus detection in germplasm certification programs. However, costs have impeded its implementation as a routine diagnostic tool. In this study, the targeted genome (TG-Seq) approach was developed to simultaneously detect multiple (four) viral species of; Pea early browning (PEBV), Cucumber mosaic (CMV), Bean yellow (BYMV) and seedborne (PSbMV). TG-Seq detected all expected amplicons within multiplex PCR (mPCR) reactions. contrast,...
ABSTRACT We present here the first complete genomic RNA sequence of polerovirus Pepper vein yellows virus (PeVYV) obtained from a pepper plant in Australia. compare it with PeVYV genomes Japan and China. The Australian genome was more closely related to Japanese than Chinese genome.
Abstract Surveys of key cucurbit production regions in New South Wales, Australia were undertaken to catalogue the diversity viral pathogens affecting field grown cropping systems. Analysis collected isolates confirmed a detection watermelon crinkle leaf associated virus-1 (WCLaV-1) constituting first report WCLaV-1 Australia. A mixed infection and mosaic virus (WMV) was also detected this type pathogen combination.
ABSTRACT We present here the first complete Squash leaf curl China virus (SLCCV) genomic segment DNA-A sequence from East Timor. It was isolated a pumpkin plant. When compared with 15 SLCCV genome sequences other world regions, it most resembled Malaysian isolate MC1 sequence.
We present here the first complete genome sequences of Sweet potato chlorotic fleck virus (SPCFV) from sweet in Australia and East Timor, we compare these with four SPCFV genomes South Korea one Uganda. The Australian, Timorese, Korean, Ugandan differed considerably each other.
ABSTRACT We present here the first complete genomic RNA sequence of polerovirus Suakwa aphid-borne yellows virus (SABYV), from East Timor. The isolate sequenced came a virus-infected pumpkin plant. Timorese genome had nucleotide identity 86.5% with only other SABYV available, which is Taiwan.
Australia is a major grain exporter, and this trade makes an important contribution to its economy. Fortunately, it remains free of many damaging virus diseases vectors found elsewhere. However, crop biosecurity under increasing pressure from global ecological, climatic, demographic challenges. Stringent plant health programs safeguard Australian production vector incursions entering via different pathways. These formerly relied upon traditional testing procedures (indicator hosts, serology,...
ABSTRACT Analysis of an RNA-seq library from cucumber leaf RNA extracted a fast technology for analysis nucleic acids (FTA) card revealed the first complete genome Cucurbit aphid-borne yellows virus (CABYV) East Timor. We compare it with 35 CABYV genomes other world regions. It most resembled South Korean isolate HD118.
Sweet potato feathery mottle virus (SPFMV) and C (SPVC) isolates were obtained from sweetpotato shoot or tuberous root samples three widely separated locations in Australia's tropical north (Cairns, Darwin, Kununurra). The planted the glasshouse scions plants graft inoculated to Ipomoea setosa plants. Virus symptoms recorded field Kununurra glasshouse-grown I. RNA extracts leaf subjected high-throughput sequencing. New complete SPFMV (n = 17) SPVC 6) genomic sequences compared with 47...