The community structure and diversity of endophytic bacteria in reed (Phragmites australis) roots growing the Beijing Cuihu Wetland, China was investigated using 16S rRNA library technique. Primers 799f 1492r were used to amplify specific bacterial fragments successfully construct clone library. In total, 166 individual sequences verified by colony PCR assess roots. Phylogenetic analysis revealed that 78.9% clones affiliated with Proteobacteria included all five classes. Other belonged...

In this study, whole genome sequencing and comparative genomic analyses were performed for Mucilaginibacter polytrichastri RG4-7 its carboxymethyl cellulose degradation potential was assessed. The results showed that the of strain 5.84 Mb contained 5019 predicted genes, in which a high proportion strain-specific genes related to carbohydrate metabolism. (CMC) cellulase activity tests revealed strong ability, CMCase β-glucosidase RG4-7. Real-time RT-PCR testing most glycoside hydrolase (GH)...

A Gram-staining-negative, rod-shaped, non-spore-forming bacterium, designated strain RG4-7 T , was isolated from the moss Polytrichastrum formosum collected Gawalong glacier in Tibet, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of were summed feature 3 (C 16 : 1 ω7 c and/or C ω6 ), iso-C 15 0 17 3-OH. major polar lipids phosphatidylethanolamine, one unidentified aminophospholipid, phospholipid, two aminolipids lipid. Strain contained MK-7 as...

A Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain xj7(T), was isolated from roots of Typha angustifolia L. growing in Beijing Cuihu Wetland, China. The isolate identified as a member the genus Paenibacillus based on phenotypic characteristics phylogenetic inference. novel spore-forming, motile, catalase-positive oxidase-negative. Optimal growth xj7(T) occurred at 28-30 °C pH 7.0-7.5. Diphosphatidylglycerol most abundant polar lipid along with...

Investigating the endophytic bacterial community in special moss species is fundamental to understanding microbial-plant interactions and discovering bacteria with stresses tolerance. Thus, structure of xerophilous Grimmia montana were estimated using a 16S rDNA library traditional cultivation methods. In total, 212 sequences derived from used assess diversity. Sequence alignment showed that endophytes assigned 54 genera 4 phyla (Proteobacteria, Firmicutes, Actinobacteria...

Previous studies on the bacteria associated with bryophytes showed that there were abundant inhabited in/on these hosts. However, type of and whether discriminate between different based a particular factor remains largely unknown.This study was designed to analyze biodiversity community ten liverworts mosses using Illumina-sequencing techniques bacterial 16S rRNA gene. A total 125,762 high quality sequences 437 OTUs obtained from twenty bryophytes. Generally, no obvious differences richness...

To understand the differences of bacteria associated with different mosses, a phylogenetic study bacterial communities in three mosses was carried out based on 16S rDNA and rRNA sequencing. The used were Hygroamblystegium noterophilum, Entodon compressus Grimmia montana, representing hygrophyte, shady plant xerophyte, respectively. In total, operational taxonomic units (OTUs), richness diversity regardless moss species library level. All examined 1183 clones assigned to 248 OTUs, 56 genera...

A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain RG1-1 T , was isolated from Takakia lepidozioides collected Gawalong glacier in Tibet, China, characterized by using a polyphasic taxonomic approach. The predominant fatty acids of were iso-C 15 : 0 (19.8 %), summed feature 3 (C 16 1 ω7 c and/or C ω6 17.0 (9.9 %) 17 3-OH (9.4 %); its major polar lipids phosphatidylethanolamine, four unidentified aminolipids, one phospholipid, aminoglycolipid, glycolipid,...

A Gram-staining-negative, rod-shaped and non-spore-forming bacterium, designated strain 333-1-0411 T , was isolated from a soil sample collected Namucuo, Tibet Autonomous Region, China characterized in taxonomic study using polyphasic approach. The major fatty acid components of were summed feature 3 (C 16 : 1 ω7 c and/or C ω6 ) 0 ; its polar lipids phosphatidylethanolamine, diphosphatidylglycerol phosphatidylglycerol. Q-8 the dominant ubiquinone, G+C content genomic DNA 66.7 mol%....

A Gram-staining-negative, rod-shaped, non-spore-forming bacterium, designated strain R27(T), was isolated from the moss Grimmia montana, collected Beijing Songshan National Nature Reserve, China, and characterized by using a polyphasic taxonomic approach. The predominant fatty acids of R27(T) were C18:1ω7c (33.6%), C16:0 (16.3%), summed feature 3 (C16:1ω7c and/or C16:1ω6c; 15.8%) C17:0 cyclo (8.7%) its major polar lipids phosphatidylethanolamine, phosphatidylglycerol, three uncharacterized...

A Gram-staining-negative bacterium, designated strain BLN2(T), was isolated from within the roots of reeds (Phragmites australis) in Beijing Cuihu Wetland (China) and characterized using a polyphasic taxonomic approach. The cells were yellow-pigmented, rod-shaped, strictly aerobic devoid flagella, but showed gliding motility. Strain BLN2(T) produced yellow, translucent, circular convex colonies, with optimal growth at 30 °C pH 7.0. major respiratory quinone menaquinone 6 (MK-6) predominant...

A Gram-stain-negative, rod-shaped and non-endospore-forming bacterium, designated strain AG1-2 T , was isolated from Takakia lepidozioides collected the Gawalong glacier in Tibet, China characterized using a polyphasic taxonomic approach. The predominant fatty acids of were iso-C 15 : 0 (36.0 %), 17 3-OH (20.2 summed feature 9 (iso-C 1 ω9 c and/or C 16 10-methyl, 16.4 %) 3 (C ω7 ω6 11.1 %). major polar lipids phosphatidylethanolamine, three unidentified aminolipids two lipids. Strain...

Assessment of the bacterial diversity associated with a decaying fern, Athyrium wallichianum Ching, revealed presence novel strain named M46T. It was Gram-stain-negative, rod-shaped, non-motile and aerobic cellulose xylan degradation abilities. Phylogenetic analysis based on 16S rRNA gene sequences showed that M46T affiliated to genus Sphingobacterium, exhibiting highest sequence similarity 97.9 % Sphingobacterium ginsenosidimutans THG 07T, canadense CR11T detergens6.2 ST. Multilocus (MLSA)...

A Gram-stain-positive, facultatively anaerobic and rod-shaped bacterium, designated strain XB T , was isolated from Physcomitrella patens growing in Beijing, China. The isolate identified as a member of the genus Paenibacillus based on phenotypic characteristics phylogenetic inferences. novel spore-forming, motile, catalase-negative weakly oxidase-positive. Optimal growth occurred at 28°C pH 7.0–7.5. major polar lipids contained diphosphatidylglycerol, phosphatidylglycerol,...

A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated strain XAT, was isolated from the moss, Physcomitrella patens, characterized by using a polyphasic taxonomic approach. The novel non-spore-forming, non-motile, catalase-positive oxidase-negative. Its optimal temperature for growth occurred at 28-30 °C optimum pH 7.0-7.5. major fatty acids comprised anteiso-C15 : 0, iso-C16 ω-cyclohexyl-C17 0 anteiso-C17 0. polar lipids contained diphosphatidylglycerol,...