A5055289374- Photoacoustic and Ultrasonic Imaging
- Ultrasound and Hyperthermia Applications
- Advanced Fluorescence Microscopy Techniques
- Ultrasound Imaging and Elastography
- Ultrasound and Cavitation Phenomena
- Near-Field Optical Microscopy
- Digital Holography and Microscopy
- Nanoplatforms for cancer theranostics
- Advanced X-ray Imaging Techniques
- Photonic and Optical Devices
- Advanced Electron Microscopy Techniques and Applications
- Ultrasonics and Acoustic Wave Propagation
- Advanced MRI Techniques and Applications
- 3D Printing in Biomedical Research
- Semiconductor Lasers and Optical Devices
- Gold and Silver Nanoparticles Synthesis and Applications
- Optical measurement and interference techniques
- Cancer Research and Treatments
- Cell Image Analysis Techniques
- Image Processing Techniques and Applications
- Carbon and Quantum Dots Applications
- Advanced Biosensing Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- RNA Interference and Gene Delivery
- Advanced Fiber Optic Sensors
Tel Aviv University
2021-2025
Stanford University
2018-2021
University of California, Davis
2017-2020
Bar-Ilan University
2012-2019
Palo Alto University
2018-2019
University of Utah
2015
The recent development of ultrasound localization microscopy, where individual microbubbles (contrast agents) are detected and tracked within the vasculature, provides new opportunities for imaging vasculature entire organs with a spatial resolution below diffraction limit. In stationary tissue, studies have demonstrated theoretical on order microns. this work, single were localized in vivo rat kidney using dedicated high frame rate sequence. Organ motion was by assuming rigid (translation...
Robust cytotoxic T cell infiltration has proven to be difficult achieve in solid tumors. We set out develop a flexible protocol efficiently transfect tumor and stromal cells produce immune-activating cytokines, thus enhance while debulking mass. By combining ultrasound with tumor-targeted microbubbles, membrane pores are created facilitate controllable local transfection. Here, we applied substantially lower transmission frequency (250 kHz) than previously. The resulting microbubble...
Abstract High intensity focused ultrasound (HIFU) rapidly and non-invasively destroys tumor tissue. Here, we sought to assess the immunomodulatory effects of MR-guided HIFU its combination with innate immune agonist CpG checkpoint inhibitor anti-PD-1. Mice multi-focal breast cancer underwent ablation a parameter set designed achieve mechanical disruption minimal thermal dose or protocol in which temperature reached 65 °C. received either alone were primed toll-like receptor 9 modulator Both...
Scaling down the size of microbubble contrast agents to nanometer level holds promise for noninvasive cancer therapy. However, small nanobubbles limits obtained bioeffects as a result ultrasound cavitation, when operating near nanobubble resonance frequency. Here we show that coupled with low energy insonation at frequency 80 kHz, well below these agents, serve therapeutic warheads trigger potent mechanical effects in tumors following systemic injection. We demonstrate capabilities tissue...
Blood brain barrier disruption (BBBD) using focused ultrasound (FUS) and microbubbles (MB) is an effective tool for therapeutic delivery to the brain. BBBD depends a great extent on MB oscillations. Because vasculature heterogenic in diameter, reduced oscillations smaller blood vessels, together with lower number of MBs capillaries, can lead variations BBBD. Therefore, evaluating impact microvasculature diameter importance. We present method characterize molecules extravasation following...
Microbubble contrast agents are widely used in ultrasound imaging and therapy, typically with transmission center frequencies the MHz range. Currently, an frequency near 250 kHz is proposed for clinical trials which combined microbubble applied to open blood brain barrier, since at this low focusing through human skull a predetermined location can be performed reduced distortion attenuation compared higher frequencies. However, vibrational response has not yet been carefully evaluated (an...
Structured illumination microscopy is an optical method to increase the spatial resolution of wide-field fluorescence imaging beyond diffraction limit by applying a spatially structured light. Here, we extend this concept facilitate super-resolution ultrasound manipulating transmitted sound field encode high frequencies into observed image through aliasing. Post processing applied precisely shift spectral components their proper positions in
Ionizable Lipid Nanoparticles (LNPs) are an FDA-approved non-viral RNA delivery system, though their use for brain therapy is restricted by the blood-brain barrier (BBB). Focused ultrasound combined with microbubbles can disrupt BBB, but delivering large particles requires balancing increased peak negative pressures while maintaining microvascular integrity. Herein, we optimized low-frequency parameters to induce high-amplitude microbubble oscillations, enabling safe of LNPs across BBB....
Abstract Objective. To develop a model that accurately describes the behavior of nanobubbles (NBs) under low-frequency ultrasound (US) insonation (<250 kHz), addressing limitations existing numerical models, such as Rayleigh-Plesset equation and Blake's Threshold model, in predicting NB behavior.&#xD;Approach. A modified surface tension derived from empirical data, was introduced to capture NBs function bubble radius. This integrated into Marmottant framework combined with Blake...
Immune checkpoint inhibitors hold promise, yet their efficacy in solid tumors is limited by the complex tumor microenvironment and lack of immune cell infiltration. This study aims to enhance immunotherapy combining anti PD-1 inhibition therapy with nanodroplet-mediated histotripsy. The proposed method involves systemic injection nanodroplets, which accumulate within tumors. These nanodroplets are then activated into cavitating gas bubbles using a rotating imaging probe, followed...
Noninvasive ultrasound surgery can be achieved using focused to locally affect the targeted site without damaging intervening tissues. Mechanical ablation and histotripsy use short intense acoustic pulses destroy tissue via a purely mechanical effect. Here, we show that coupled with low-frequency excitation, microbubbles serve as therapeutic warheads trigger potent effects in tumors ultrasound. Upon low frequency excitation (250 kHz below), high amplitude microbubble oscillations occur at...
Ultrasound insonation of microbubbles can be used to form pores in cell membranes and facilitate the local trans-membrane transport drugs genes. An important factor efficient delivery is size delivered target compared generated membrane pores. Large molecule remains a challenge, affect resulting therapeutic outcomes. To large delivery, need formed. While ultrasound typically uses megahertz frequencies, it was recently shown that when are excited at frequency 250 kHz (an order magnitude below...
Nanoscale ultrasound contrast agents show promise as alternatives for diagnostics and therapies due to their enhanced stability ability traverse blood vessels. Nonetheless, reduced size limits echogenicity. This study introduces an nanobubble frequency mixing imaging method, by capitalizing on nonlinear acoustic response dual-frequency excitation.
Utilizing the surface plasmon resonance effect in gold nanoparticles enables their use as contrast agents a variety of applications for compound cellular imaging. However, most techniques suffer from poor signal to noise ratio (SNR) statistics due high shot that is associated with low photon count addition background noise. We demonstrate an effective way improve SNR, particular when inspected indistinguishable given noisy environment. excite temporal flickering scattered light nanoparticle...
Abstract Low pressure histotripsy is likely to facilitate current treatments that require extremely high pressures. An ultrasound guided focused system was designed accommodate a rotating imaging transducer within low frequency therapeutic operates at center of 105 kHz. The implementation this integrated provides real-time and volumetric functions, are used here for low-cost, low-energy 3D using nanodroplets. A two-step approach implemented with dual-array. Vaporization nanodroplets into...
Acoustical hologram generation can be achieved via controlled beam shaping by engineering the transmitted phases to create a desired pattern. Optically inspired phase retrieval algorithms and standard methods assume continuous wave (CW) insonation, which successfully generate acoustic holograms for therapeutic applications that involve long burst transmissions. However, technique designed single-cycle transmission capable of achieving spatiotemporal interference pulses is needed imaging...
This paper presents a novel optical system for the realization of Radon transform in single frame. The is simple, fast and accurate consists 4F system, where 2F plane vortex like element placed. performs rotation object, which replaces need mechanically rotating it, as done other common techniques transform. realized using spatial light modulator (SLM) an amplitude slide. obtained given Cartesian coordinates, can subsequently be transformed computer to polar set. proposed concept supported...
We developed an improved sonoporation method using nanobubbles, offering potential for noninvasive uses. Our results confirmed that low frequency insonation yields outcomes comparable to those achieved with targeted microbubbles.
Abstract In this paper we present a technique aimed for simultaneous detection of multiple types gold nanoparticles (GNPs) within biological sample, using lock-in detection. We image the sample number modulated laser beams that correspond to GNP species label given sample. The final where GNPs are spatially separated is obtained computationally. proposed method enables superresolved imaging different areas interest and also spatial separation at sub-diffraction distances, making it useful...
This paper presents a method for modifying the point spread function (PSF) into doughnut-like shape, through utilization of plasma dispersion effect (PDE) silicon-coated gold nanoparticles. modified PSF has spatial components smaller than diffraction limit, and by scanning sample with it, super-resolution can be achieved. The is illuminated using two laser beams. first pump, wavelength in visible region that creates change refractive index silicon coating due to PDE. localized surface...
Super-resolution localization microscopy has revolutionized the observation of living structures at cellular scale, by achieving a spatial resolution that is improved more than an order magnitude compared to diffraction limit. These methods localize single events from isolated sources in repeated cycles achieve super-resolution. The requirement for sparse distribution simultaneously activated field view dictates acquisition thousands frames construct full super-resolution image. As result,...
Nanodroplets are phase-changing agents that have shown great potential for ultrasound applications. When is applied, nanodroplets can undergo a phase transition into gas bubbles, enabling cavitation be used to reduce the pressure threshold required mechanical ablation of tissues. Effective tissue fractionation depends on precise vaporization achieve uniform and predictable bubble formation. This study aimed optimize nanodroplet using acoustic holograms improved nanodroplet-mediated...