A5036741552- Mesenchymal stem cell research
- Bone fractures and treatments
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Tendon Structure and Treatment
- Spine and Intervertebral Disc Pathology
- Virus-based gene therapy research
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Ultrasound and Hyperthermia Applications
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Osteoarthritis Treatment and Mechanisms
- Orthopaedic implants and arthroplasty
- Biomedical Ethics and Regulation
- Photoacoustic and Ultrasonic Imaging
- Cellular Mechanics and Interactions
- Microbial Inactivation Methods
- Knee injuries and reconstruction techniques
- Bone Metabolism and Diseases
- Bone health and osteoporosis research
- Shoulder Injury and Treatment
- Spaceflight effects on biology
- Animal Genetics and Reproduction
- Lanthanide and Transition Metal Complexes
Hebrew University of Jerusalem
2012-2022
Cedars-Sinai Medical Center
2013-2022
Stanford University
2019
Hadassah Medical Center
2001-2017
Regenerative Medicine Institute
2015-2017
Pennington Biomedical Research Center
2009
Louisiana State University System
2009
Colorado State University
2009
University of Southampton
2003
University of Iowa
2003
Regulated expression of transgene production and function is great importance for gene therapy. Such regulation can potentially be used to monitor control complex biological processes. We report here a regulated stem cell-based system controlling bone regeneration, utilizing genetically engineered mesenchymal cells (MSCs) harboring tetracycline-regulated vector encoding the osteogenic growth factor human BMP-2. show that doxycycline (a tetracycline analogue) able hBMP-2 thus MSC...
Background Human mesenchymal stem cells (hMSCs) are pluripotent that can differentiate to various cell types. Recently, a method isolate hMSCs from bone marrow and expand them in culture was described. Here we report on the use of as platform for gene therapy aimed at lesions. Methods Bone derived were expanded infected with recombinant adenoviral vector encoding osteogenic factor, human BMP-2. The potential genetically engineered assessed vitro vivo. Results Genetically displayed enhanced...
Microbubble-enhanced, ultrasound-mediated BMP-6 gene delivery to endogenous progenitor cells induces rapid and efficient repair of critical-sized, nonunion bone fractures in mini-pigs.
Abstract Mesenchymal stem cells (MSCs) are currently the most established for skeletal tissue engineering and regeneration; however, their availability capability of self-renewal limited. Recent discoveries somatic cell reprogramming may be used to overcome these challenges. We hypothesized that induced pluripotent (iPSCs) were differentiated into MSCs could bone regeneration. Short-term exposure embryoid bodies transforming growth factor-β was direct iPSCs toward MSC differentiation. During...
Robust cytotoxic T cell infiltration has proven to be difficult achieve in solid tumors. We set out develop a flexible protocol efficiently transfect tumor and stromal cells produce immune-activating cytokines, thus enhance while debulking mass. By combining ultrasound with tumor-targeted microbubbles, membrane pores are created facilitate controllable local transfection. Here, we applied substantially lower transmission frequency (250 kHz) than previously. The resulting microbubble...
Viral delivery of the therapeutic gene bone morphogenetic protein-2 (BMP-2) is a promising approach for regeneration. The human parvovirus adeno-associated virus (AAV) type 2 considered one most encouraging viral vector systems because its high transduction rates and biosafety ratings. Bone highly potent osteoinductive protein, which induces formation in vivo osteogenic differentiation vitro. exogenous regulation BMP-2 expression bone-regenerating sites required to control protein secretion,...
Abstract Multiple factors alter intervertebral disc volume, structure, shape, composition, and biomechanical properties, often leading to low back pain. Spinal fusion is frequently performed treat this problem. We recently published results of our investigation a novel system in vivo bone formation, which we used nonvirally nucleofected human mesenchymal stem cells that overexpress morphogenetic protein gene. hypothesized primary porcine adipose tissue-derived (ASCs) with plasmid containing...
The presence of live periosteal progenitor cells on the surface bone autografts confers better healing than devitalized allograft. We have previously demonstrated in a murine 4 mm segmental femoral bone-grafting model that periosteum produces robust endochondral and intramembraneous formation is essential for effective neovascularization structural grafts. To end engineering pseudo-periosteum could induce similar response onto allograft, we seeded mesenchymal stem cell line stably...
Vertebral compression fractures (VCFs), the most common fragility fractures, account for approximately 700,000 injuries per year. Since open surgery involves morbidity and implant failure in osteoporotic patient population, a new minimally invasive biological solution to vertebral bone repair is needed. Previously, we showed that adipose-derived stem cells (ASCs) overexpressing BMP gene are capable of inducing spinal fusion vivo. We hypothesized direct injection ASCs, designed transiently...
The genes encoding the core circadian transcription factors display an oscillating expression profile in murine calvarial bone. More than 26% of bone transcriptome exhibits a rhythm, comparable with that observed brown and white adipose tissues liver. Thus, mechanisms may directly modulate oxidative phosphorylation multiple metabolic pathways homeostasis.Although rhythms have been associated historically central regulatory mechanisms, there is emerging evidence transcriptional apparatus...
Nonunion fractures present a challenge to orthopedics with no optimal solution. In-vivo DNA electroporation is gene-delivery technique that can potentially accelerate regenerative processes. We hypothesized in vivo of an osteogenic gene nonunion radius bone defect site would induce fracture repair. was created the radii C3H/HeN mice, into which collagen sponge placed. To allow for recruitment host progenitor cells (HPCs) implanted sponge, mice were housed 10 days before electroporation. Mice...